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Investigation of the interaction of GdnHCl with poly-L-lysine, horseradish peroxidase and cytochrome c monitored via FTIR, CD and fluorescence spectroscopies

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Investigation of the interaction of GdnHCl with poly-L-lysine, horseradish peroxidase and cytochrome c monitored via FTIR, CD and fluorescence spectroscopies

Esposito, Christina (2000) Investigation of the interaction of GdnHCl with poly-L-lysine, horseradish peroxidase and cytochrome c monitored via FTIR, CD and fluorescence spectroscopies. Masters thesis, Concordia University.

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Abstract

Spectroscopic probes were utilized to study the interaction of GdnHCl with three model systems: poly-L-lysine (PLL), horseradish peroxidase (HRP) and cytochrome c. Probing the chemically denatured states of proteins by FTIR is a novel technique, which has only recently been reported. To aid in the interpretation of the FTIR results, fluorescence and CD were employed. The use of deuterated solvents, i.e. Gdn-d 5 -DCl and D 2 O, simplified data processing and is recommended for FTIR studies of protein chemical denaturation. PLL, a random coil, was used to model the interaction between Gdn-d 5 -DCl and random structures. Spectroscopic evidence strongly suggested that Gdnd 5 -DCl associates with PLL, particularly with its lysyl side chains. The analysis of HRP indicated that it was not a suitable model due to complex time-dependent spectral changes. The study of cytochrome c in Gdn-d 5 -DCl revealed that the denaturant preferentially interacted with random structures. The denatured states of horse and tuna c were characterized and subtle differences between the two species were reported. Curvefitting of the FTIR amide I ' mode (1600-1700 cm -1 ) revealed that tuna c contained more residual structure in Gdn-d 5 -DCl than horse c. It was successfully demonstrated that FTIR is a powerful tool for probing the chemically denatured states of model protein systems, with potential applications for other biomolecules.

Divisions:Concordia University > Faculty of Arts and Science > Chemistry and Biochemistry
Item Type:Thesis (Masters)
Authors:Esposito, Christina
Pagination:xi, 137 leaves : ill. ; 29 cm.
Institution:Concordia University
Degree Name:Theses (M.Sc.)
Program:Chemistry and Biochemistry
Date:2000
Thesis Supervisor(s):English, Ann M.
ID Code:1030
Deposited By:Concordia University Libraries
Deposited On:27 Aug 2009 13:16
Last Modified:08 Dec 2010 10:18
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