Breadcrumb

 
 

In vivo studies of CRP and LRP in the regulation of sdaA, serA and metK genes in Escherichia coli K-12

Title:

In vivo studies of CRP and LRP in the regulation of sdaA, serA and metK genes in Escherichia coli K-12

Ding, Jie Ping (2002) In vivo studies of CRP and LRP in the regulation of sdaA, serA and metK genes in Escherichia coli K-12. Masters thesis, Concordia University.

[img]
Preview
PDF
2607Kb

Abstract

The sdaA gene of Escherichia coli encodes L-serine deaminase (L-SD), which degrades L-serine to ammonia and pyruvate. LRP represses the expression of both L-SD and sdaA . CRP was assumed to affect sdaA transcription, but this had not been proven. I demonstrated here that both CRP and LRP were involved in L-SD and sdaA expression. LRP repressed L-SD expression and sdaA transcription. CRP activated L-SD expression depending on the presence of LRP but did not affect sdaA transcription. The genes for serA and metK are known to be regulated by LRP and are proposed to be regulated by CRP. CRP activated the serA P2 promoter. However, it did not affect serA expression overall. CRP was also involved in the regulation of metK expression through an unclear mechanism. By providing ptsG expression on a plasmid, I identified one factor causing the slow growth rate of crp mutants. The rate of glucose uptake was suggested to affect the growth rate of crp mutants.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Thesis (Masters)
Authors:Ding, Jie Ping
Pagination:xii, 96 leaves : ill. ; 29 cm.
Institution:Concordia University
Degree Name:Theses (M.Sc.)
Program:Biology
Date:2002
Thesis Supervisor(s):Newman, Elaine
ID Code:2430
Deposited By:Concordia University Libraries
Deposited On:27 Aug 2009 13:28
Last Modified:08 Dec 2010 10:26
Related URLs:
All items in Spectrum are protected by copyright, with all rights reserved. The use of items is governed by Spectrum's terms of access.

Repository Staff Only: item control page

Document Downloads

More statistics for this item...

Concordia University - Footer