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Isolation and partial characterization of cDNAs encoding polypeptides that interact with the capB gene products of Dictyostelium discoideum

Title:

Isolation and partial characterization of cDNAs encoding polypeptides that interact with the capB gene products of Dictyostelium discoideum

Martinez-Perez, Amalia (1997) Isolation and partial characterization of cDNAs encoding polypeptides that interact with the capB gene products of Dictyostelium discoideum. Masters thesis, Concordia University.

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Abstract

The gene products of capB, p34 and p31, were previously isolated by co-purification with the cAMP binding protein CABP1 encoded by the capB homolog capA. The GAL4 two-hybrid system was used to screen a Dictyostelium discoideum cDNA expression library to identify proteins that interacted with p34/31. The clones were tested to eliminate false positives. DNA sequencing analysis revealed that the cDNAs isolated encoded: p34/31; CABP1B, the smaller product of capA; TopA, a mitochondrial type II topoisomerase; and a previously unidentified polypeptide (clone 101) with homology to a bacterial ORF. It was also determined that the product of clone 101 interacted with the gene products of capA in the two-hybrid system. The interactions between p34/31 and TopA, coupled with the results of previous mutation and localization studies, suggest a role for these polypeptides in mitochondrial function. Moreover the isolation of cDNAs encoding CABP1B and the detection of interactions between polypeptide 101 and the capA gene products may imply that p34/31 have more than one intracellular role.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Thesis (Masters)
Authors:Martinez-Perez, Amalia
Pagination:xi, 70 leaves : ill. ; 29 cm.
Institution:Concordia University
Degree Name:Theses (M.Sc.)
Program:Biology
Date:1997
Thesis Supervisor(s):Tsang, Adrian S
ID Code:381
Deposited By:Concordia University Libraries
Deposited On:27 Aug 2009 13:11
Last Modified:08 Dec 2010 10:14
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