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Isolation and sequencing of a genomic clone of ESI28 : an early salt-stress induced gene of the salt-tolerant wild wheatgrass Lophopyrum elongatum

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Isolation and sequencing of a genomic clone of ESI28 : an early salt-stress induced gene of the salt-tolerant wild wheatgrass Lophopyrum elongatum

Amani., Abu-Idrees (1998) Isolation and sequencing of a genomic clone of ESI28 : an early salt-stress induced gene of the salt-tolerant wild wheatgrass Lophopyrum elongatum. Masters thesis, Concordia University.

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Abstract

A genomic sequence corresponding to a putative actin-depolymerizing factor (ADF) was isolated from a wheat (Lophopyrum elongatum) genomic library. The genomic clone, designated ESI28-G-5, codes for the ESI28 gene (early salt-stress induced). The 5$\sp\prime$-upstream sequence contains a putative TATA box and abscisic acid (ABA) response element (ABRE). The genomic clone contains a long open reading frame (ORF) which encodes a polypeptide of 143 amino acids with a predicted molecular mass of 15.95 kDa. The protein has two conserved domains identified as actin and phosphatidylinositol 4,5-biphosphate (PIP2) binding sites which are commonly found in members of the cofilin family. A search of DNA sequence databases indicated that the ESI28 sequence had the highest degree of sequence similarity to the Triticum aestivum low temperature regulated gene (Wcor719) which encodes an actin-binding protein (ABP). The induction of ESI28 by salt, the presence of ABRE in its promoter region, and the significant homology with ABP from different organisms suggest that this gene might be involved in reorganizing the cytoskeleton during water deficit stress. It may also serve as an essential factor in the salt stress signal transduction pathway that is regulated by ABA.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Thesis (Masters)
Authors:Amani., Abu-Idrees
Pagination:xi, 61 leaves : ill. ; 29 cm.
Institution:Concordia University
Degree Name:Theses (M.Sc.)
Program:Biology
Date:1998
Thesis Supervisor(s):Gulick, Patrick J.
ID Code:569
Deposited By:Concordia University Libraries
Deposited On:27 Aug 2009 13:12
Last Modified:08 Dec 2010 10:15
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