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Microsatellite DNA markers in Populus tremuloides

Title:

Microsatellite DNA markers in Populus tremuloides

Rahman, Muhammad H. and Dayanandan, Selvadurai and Rajora, Om P. (2000) Microsatellite DNA markers in Populus tremuloides. Genome, 43 (2). pp. 293-297. ISSN 0831-2796

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Official URL: http://dx.doi.org/10.1139/gen-43-2-293

Abstract

Markers for eight new microsatellite DNA or simple sequence repeat (SSR) loci were developed and characterized in trembling aspen (Populus tremuloides) from a partial genomic library. Informativeness of these microsatellite DNA markers was examined by determining polymorphisms in 38 P. tremuloides individuals. Inheritance of selected markers was tested in progenies of controlled crosses. Six characterized SSR loci were of dinucleotide repeats (two perfect and four imperfect), and one each of trinucleotide and tetranucleotide repeats. The monomorphic SSR locus (PTR15) was of a compound imperfect dinucleotide repeat. The primers of one highly polymorphic SSR locus (PTR7) amplified two loci, and alleles could not be assigned to a specific locus. At the other six polymorphic loci, 25 alleles were detected in 38 P. tremuloides individuals; the number of alleles ranged from 2 to 7, with an average of 4.2 alleles per locus, and the observed heterozygosity ranged from 0.05 to 0.61, with an average of 0.36 per locus. The two perfect dinucleotide and one trinucleotide microsatellite DNA loci were the most informative. Microsatellite DNA variants of four SSR loci characterized previously followed a single-locus Mendelian inheritance pattern, whereas those of PTR7 from the present study showed a two-locus Mendelian inheritance pattern in controlled crosses. The microsatellite DNA markers developed and reported here could be used for assisting various genetic, breeding, biotechnology, genome mapping, conservation, and sustainable forest management programs in poplars.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Article
Refereed:Yes
Authors:Rahman, Muhammad H. and Dayanandan, Selvadurai and Rajora, Om P.
Journal or Publication:Genome
Date:April 2000
Funders:
  • Natural Sciences and Engineering Research Council of Canada
Keywords:poplar, microsatellites, genetic mapping, simple sequence repeat (SSR) markers, DNA fingerprinting.
ID Code:6709
Deposited By:DANIELLE DENNIE
Deposited On:09 Jun 2010 18:16
Last Modified:08 Dec 2010 18:17
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