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Characterization of a monoclonal antibody specific to a flavonol 2′-O-glucosyltransferase

Title:

Characterization of a monoclonal antibody specific to a flavonol 2′-O-glucosyltransferase

Latchinian, Lilian and Ibrahim, Ragai K. (1989) Characterization of a monoclonal antibody specific to a flavonol 2′-O-glucosyltransferase. Biochemistry and Cell Biology, 67 (4-5). pp. 210-213. ISSN 0829-8211

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Official URL: http://dx.doi.org/10.1139/o89-032

Abstract

A monoclonal antibody to a partially purified preparation of 2′-O-glucosyltransferase was produced by in vitro immunization of spleen cells from BALB/c mice, followed by fusion with mouse myeloma cells. Hybridoma culture supernatants were screened by enzyme-linked immunosorbent assay for (i) their ability to produce immunoglobulins and (ii) their immunoreactivity with a partially purified enzyme preparation. The majority of the immunoglobulin-producing hybridomas were IgM secretors. Two highly immunoreactive IgM-secreting clones were chosen for further characterization. The supernatant fraction from a culture of one of these clones displayed 50% inhibition of the 2′-O-glucosyltransferase activity. The native form of the 2′-O-glucosyltransferase was essential for recognition, suggesting that the epitope recognized by the antibody is a conformational discontiguous one.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Article
Refereed:Yes
Authors:Latchinian, Lilian and Ibrahim, Ragai K.
Journal or Publication:Biochemistry and Cell Biology
Date:1989
Funders:
  • Natural Sciences and Engineering Research Council of Canada
  • Department of Education, Government of Quebec
Keywords:monoclonal antibody, in vitro immunization, flavonoid, O-glucosyltransferase
ID Code:6775
Deposited By:DANIELLE DENNIE
Deposited On:13 Jul 2010 11:29
Last Modified:08 Dec 2010 18:07
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