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DNA Asymmetric Strand Bias Affects the Amino Acid Composition of Mitochondrial Proteins


DNA Asymmetric Strand Bias Affects the Amino Acid Composition of Mitochondrial Proteins

Mi, Xiang Jia and Hickey, Donal A. (2007) DNA Asymmetric Strand Bias Affects the Amino Acid Composition of Mitochondrial Proteins. DNA Research, 14 (5). pp. 201-206. ISSN 1340-2838

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Hickey_DNAResearch2007.pdf - Published Version

Official URL: http://dx.doi.org/10.1093/dnares/dsm019


Variations in GC content between genomes have been extensively documented. Genomes with comparable GC contents can, however, still differ in the apportionment of the G and C nucleotides between the two DNA strands. This asymmetric strand bias is known as GC skew. Here, we have investigated the impact of differences in nucleotide skew on the amino acid composition of the encoded proteins. We compared orthologous genes between animal mitochondrial genomes that show large differences in GC and AT skews. Specifically, we compared the mitochondrial genomes of mammals, which are characterized by a negative GC skew and a positive AT skew, to those of flatworms, which show the opposite skews for both GC and AT base pairs. We found that the mammalian proteins are highly enriched in amino acids encoded by CA-rich codons (as predicted by their negative GC and positive AT skews), whereas their flatworm orthologs were enriched in amino acids encoded by GT-rich codons (also as predicted from their skews). We found that these differences in mitochondrial strand asymmetry (measured as GC and AT skews) can have very large, predictable effects on the composition of the encoded proteins.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Article
Authors:Mi, Xiang Jia and Hickey, Donal A.
Journal or Publication:DNA Research
Date:1 November 2007
Digital Object Identifier (DOI):10.1093/dnares/dsm019
Keywords:mitochondrion, strand asymmetry, amino acids, codon usage
ID Code:7609
Deposited On:16 May 2011 16:25
Last Modified:04 Nov 2016 23:39


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