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Official URL: http://dx.doi.org/10.1093/dnares/dsm019
Variations in GC content between genomes have been extensively documented. Genomes with comparable GC contents can, however, still differ in the apportionment of the G and C nucleotides between the two DNA strands. This asymmetric strand bias is known as GC skew. Here, we have investigated the impact of differences in nucleotide skew on the amino acid composition of the encoded proteins. We compared orthologous genes between animal mitochondrial genomes that show large differences in GC and AT skews. Specifically, we compared the mitochondrial genomes of mammals, which are characterized by a negative GC skew and a positive AT skew, to those of flatworms, which show the opposite skews for both GC and AT base pairs. We found that the mammalian proteins are highly enriched in amino acids encoded by CA-rich codons (as predicted by their negative GC and positive AT skews), whereas their flatworm orthologs were enriched in amino acids encoded by GT-rich codons (also as predicted from their skews). We found that these differences in mitochondrial strand asymmetry (measured as GC and AT skews) can have very large, predictable effects on the composition of the encoded proteins.
|Divisions:||Concordia University > Faculty of Arts and Science > Biology|
|Authors:||Mi, Xiang Jia and Hickey, Donal A.|
|Journal or Publication:||DNA Research|
|Date:||01 November 2007|
|Keywords:||mitochondrion, strand asymmetry, amino acids, codon usage|
|Deposited By:||DANIELLE DENNIE|
|Deposited On:||16 May 2011 12:25|
|Last Modified:||06 Jun 2011 11:32|
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