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Detection of explosive compounds using Photosystem II-based biosensor


Detection of explosive compounds using Photosystem II-based biosensor

Bhalla, Vijayender and Zhao, Xin and Zazubovich, Valter (2011) Detection of explosive compounds using Photosystem II-based biosensor. Journal of Electroanalytical Chemistry, 657 (1-2). pp. 84-90. ISSN 15726657

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Official URL: http://dx.doi.org/10.1016/j.jelechem.2011.03.026


The efficacy of a Photosystem II (PS II)-based biosensor for the detection of explosive compounds has been explored. The idea is based on the close similarities in the chemical structures of the widespread explosives and herbicides, with the latter known to inhibit functioning of the PS II by attaching to the binding site of the QB mobile plastoquinone electron acceptor. The gold screen-printed electrodes (Au-SPE) functionalized with PS II-enriched particles were used for the detection of explosives in a droplet biosensor configuration. A crude preparation of PS II produced from spinach leaves, known as BBY particles, was employed to modify the Au-SPE working electrode employing BSA–glutaraldehyde-based immobilization procedure. Inhibition of the PS II functioning was detected by photo-electrochemical measurements in the presence of a mediator (either non-native quinone or ferricyanide). The biosensor was highly responsive to herbicides (as expected) as well as to picric acid, with limits of detection in the nanomolar range, but trace detection of trinitrotoluene (TNT) was not effective. The detection limit for picric acid was 25 nM as compared to 400 nM for TNT with duroquinone mediator. Low affinity of PS II to TNT has been corroborated by means of DCPIP assay; possible reasons for low affinity are discussed.

Divisions:Concordia University > Faculty of Arts and Science > Physics
Item Type:Article
Authors:Bhalla, Vijayender and Zhao, Xin and Zazubovich, Valter
Journal or Publication:Journal of Electroanalytical Chemistry
Date:1 July 2011
Keywords:Photosystem II; Herbicides; Picric acid; TNT; Screen-printed electrode
ID Code:7632
Deposited On:03 Jun 2011 18:11
Last Modified:03 Jun 2011 18:11
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