Aysola, Prasad (1998) Pulse microwave mediated sample clean-up method to analyse trace metals, PCBs and pesticides, and for treatment of organic wastes. Masters thesis, Concordia University.
A procedure is described for open vessel pulse microwave wet ashing system. In wet ashing procedure organic matrix in biological tissues is digested in acid. This step must precede trace metal analysis by atomic absorption spectroscopy. Conventional wet ashing procedure using hot plate takes several hours. Digestion of animal and plant tissues by 5.0 ml, 10.0 ml or 20.0 ml of $\rm H\sb2SO\sb4$ (95.0-98%)/HNO$\sb3$ (69-71%) (v/v) (1:1) acid mixture were effectively achieved by pulse microwave treatment using domestic grade microwave oven. Samples are subjected to 10s (method A) or 6s (method B) microwave heating followed by dormant time of 3 minutes for a total microwave heating time of less than 20 minutes. Data will be presented to show how conditions were chosen. The temperature of digestion mixture was maintained $<$100$\sp\circ$C. The effect of pulse time variation and acid mixture volume variation was studied in detail.
|Divisions:||Concordia University > Faculty of Arts and Science > Chemistry and Biochemistry|
|Item Type:||Thesis (Masters)|
|Pagination:||xx, 205 leaves : ill. ; 29 cm.|
|Degree Name:||Theses (M.Sc.)|
|Program:||Dept. of Chemistry and Biochemistry|
|Thesis Supervisor(s):||Anderson, P. D.|
|Deposited By:||Concordia University Libraries|
|Deposited On:||27 Aug 2009 17:14|
|Last Modified:||08 Dec 2010 15:16|
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