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Novel technology for sustainable petroleum oily sludge management : bio-neutralization by indigenous fungal-bacterial co-cultures

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Novel technology for sustainable petroleum oily sludge management : bio-neutralization by indigenous fungal-bacterial co-cultures

Said, Mahmoud (2004) Novel technology for sustainable petroleum oily sludge management : bio-neutralization by indigenous fungal-bacterial co-cultures. Masters thesis, Concordia University.

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Abstract

The treatment and disposal of petroleum oily sludges represent major challenges for petroleum industries. The oily sludges consume a high portion of a refiner's budget and pose a serious threat to the environment. Montreal and Kyoto protocols introduced significant restrictions on the disposal of petroleum wastes and reduced the options available for treating this type of hazardous wastes. This research considered the application of bioremediation principles to petroleum oily sludge using a new testing technique. Approximately, 35% of the total petroleum hydrocarbons, and 81% of the aliphatic hydrocarbons in the sludge were degraded using a fungal-bacterial co-culture. To my knowledge, this was the first time fungal-bacterial co-cultures have been used in the treatment of petroleum oily sludge. Prior to treatment with the co-cultures, the sludge was subjected to a special electrokinetic separation technology that reduced its oil and water contents. The cultures were isolated from petroleum oily sludge taken from the bottom of crude oil storage tanks. The fungal and bacterial strains, used in the co-culture, were identified as Paecilomyces variotii and Bacillus cereus , respectively. The cultures were inoculated on 0.22 om filters laid over the sludge. Reduction in aliphatic hydrocarbons was estimated using Fourier transform infrared spectrometry (FTIR). Total Petroleum Hydrocarbons (TPH) were estimated using solvent extraction. An amphoteric surfactant was used in the studies but it did not improve biodegradation rates. The testing technique gave a comprehensive indication of the efficiency of the process and the toxicity of the sludge for defined cultures of the microorganisms.

Divisions:Concordia University > Faculty of Engineering and Computer Science > Building, Civil and Environmental Engineering
Item Type:Thesis (Masters)
Authors:Said, Mahmoud
Pagination:xi, 104 leaves : ill. (some col.) ; 29 cm.
Institution:Concordia University
Degree Name:M.A. Sc.
Program:Building, Civil and Environmental Engineering
Date:2004
Thesis Supervisor(s):Elektorowicz, Maria
ID Code:7969
Deposited By:Concordia University Libraries
Deposited On:18 Aug 2011 14:11
Last Modified:18 Aug 2011 14:12
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