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A rapid liquid chromatography / tandem mass spectrometry assay for in vivo monitoring of extracellular monoamine neurotransmitters and their metabolites

Title:

A rapid liquid chromatography / tandem mass spectrometry assay for in vivo monitoring of extracellular monoamine neurotransmitters and their metabolites

Bablekis, Vasiliki (2004) A rapid liquid chromatography / tandem mass spectrometry assay for in vivo monitoring of extracellular monoamine neurotransmitters and their metabolites. Masters thesis, Concordia University.

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Abstract

In this study, a rapid and selective method using microdialysis liquid chromatography/tandem mass spectrometry was developed for the in vivo analysis of the catecholamine dopamine, and its metabolites DOPAC and HVA, and the indolamine serotonin, and its metabolite 5-HIAA. Sampling of the extracellular fluid by microdialysis enables continuous monitoring of intercellular chemical concentrations, which allows for the study of neuronal communications. Liquid chromatography using a Fluophase PFP column (1.0 x 50 mm, 5 om), packed with a fluorinated phase, provided sufficient retention and separation of both monoamine neurotransmitters and their respective acid metabolites. This analytical column allowed for online desalting, which was critical for successful detection, by combining hydrophilic interaction chromatography (HILIC) and reverse phase chromatography. Mass spectrometric detection was performed using electrospray ionization coupled to an AB/MDS Sciex API3000 triple quadrupole mass spectrometer operating in both negative and positive ion modes. (Abstract shortened by UMI.)

Divisions:Concordia University > Faculty of Arts and Science > Chemistry and Biochemistry
Item Type:Thesis (Masters)
Authors:Bablekis, Vasiliki
Pagination:viii, 98 leaves : ill. ; 29 cm.
Institution:Concordia University
Degree Name:M. Sc.
Program:Chemistry and Biochemistry
Date:2004
Thesis Supervisor(s):Pfaus, Jim
ID Code:7972
Deposited By:Concordia University Libraries
Deposited On:18 Aug 2011 14:12
Last Modified:18 Aug 2011 14:12
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