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Proteomic analysis of phenol degradation in soil yeast Trichosporon cutaneum

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Proteomic analysis of phenol degradation in soil yeast Trichosporon cutaneum

Man, Jun (2004) Proteomic analysis of phenol degradation in soil yeast Trichosporon cutaneum. Masters thesis, Concordia University.

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Abstract

Trichosporon cutaneum is a soil yeast that utilizes a variety of aromatic compounds as sole carbon and energy sources. T. cutaneum is perhaps the best-characterized aromatic-degrading fungus from which further information about biodegradation can be obtained. In this thesis, the global effects on the T. cutaneum proteome associated with growth on phenol have been examined. In order to identify all the enzymes involved in the phenol degradation pathway and to find other proteins associated with the degradation process that are not part of the catabolic pathway, proteomic analysis combine high-resolution two-dimensional gel electrophoresis (2-DE) and mass spectrometry. The optimized sample preparation methods and experimental conditions provide a great advantage in quantitative analysis and potential high-throughout applications. Three mass spectrometry methods, matrix-assisted laser desorption/ionization quadrupole time-of-flight mass spectrometry (MALDI-QTOF-MS), nano-electrospray mass spectrometry (nanoESI-MS/MS) and capillary liquid chromatography mass spectrometry (CapLC-MS/MS), have been successfully applied to studying proteins of T. cutaneum strain ATCC 58094 differentially expressed in response to growth on phenol. Cross-species identification provides a powerful tool in protein identification with an unknown genome and was applied to the mass spectral data obtained. Except for phenol hydroxylase and cis, cis -muconate cyclase, the other identified 24 proteins were not previously known to be associated with phenol degradation in T. cutaneum

Divisions:Concordia University > Faculty of Arts and Science > Chemistry and Biochemistry
Item Type:Thesis (Masters)
Authors:Man, Jun
Pagination:x, 81 leaves : ill. ; 29 cm.
Institution:Concordia University
Degree Name:M. Sc.
Program:Chemistry and Biochemistry
Date:2004
Thesis Supervisor(s):Powlowski, Justin
ID Code:8125
Deposited By:Concordia University Libraries
Deposited On:18 Aug 2011 14:16
Last Modified:18 Aug 2011 14:16
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