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The characterization of the glucose tolerance factor (GTF) in L6 myotubes and 3T3-L1 adipocytes

Title:

The characterization of the glucose tolerance factor (GTF) in L6 myotubes and 3T3-L1 adipocytes

Brunetti, Donato (2004) The characterization of the glucose tolerance factor (GTF) in L6 myotubes and 3T3-L1 adipocytes. Masters thesis, Concordia University.

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Abstract

This thesis reports the effects of the Glucose Tolerance Factor (GTF) on the stimulation of 2-deoxyglucose (2-DG) transport in 3T3-L1 adipocytes and in L6 myotubes. The maximal effects on 2-DG transport in adipocytes were observed at concentrations of 133 nM insulin. The maximal effect of GTF was seen at 20 mg/ml in 3T3-L1 fat cells while maximal stimulation of GTF was seen in L6 myotubes at 50 mg/ml. These observations indicated that GTF had similar effects to insulin in stimulating glucose uptake in 3T3-L1 adipocytes and in L6 myotubes. Also, GTF works in an additive manner with insulin giving a higher stimulation in glucose transport without interfering with insulin binding to its receptor. GTF's mode of action is not through the phosphorylation of the insulin receptor β chain. However, GTF seems to stimulate phosphorylation of downstream signals such as the IRS-1. (Abstract shortened by UMI.)

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Thesis (Masters)
Authors:Brunetti, Donato
Pagination:xii, 106 leaves : ill. ; 29 cm.
Institution:Concordia University
Degree Name:M. Sc.
Program:Biology
Date:2004
Thesis Supervisor(s):Germinario, Ralph
ID Code:8338
Deposited By:Concordia University Libraries
Deposited On:18 Aug 2011 14:22
Last Modified:18 Aug 2011 14:22
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