Login | Register

Characterization of O-methyltransferase (OMT)-like cDNA clones from arabidopsis thaliana and serratula tinctoria, and phylogenomic analysis of plant OMTs

Title:

Characterization of O-methyltransferase (OMT)-like cDNA clones from arabidopsis thaliana and serratula tinctoria, and phylogenomic analysis of plant OMTs

Lam, Kevin Chee Loong (2005) Characterization of O-methyltransferase (OMT)-like cDNA clones from arabidopsis thaliana and serratula tinctoria, and phylogenomic analysis of plant OMTs. Masters thesis, Concordia University.

[thumbnail of MR10205.pdf]
Preview
Text (application/pdf)
MR10205.pdf - Accepted Version
6MB

Abstract

O-Methylation of plant secondary metabolites, which plays important roles in the biosynthesis of various natural products and function of plants, is catalyzed by a superfamily of S-adenosyl- L-methionine-dependent O-methyltransferases (OMTs). The objectives of the research were to isolate and characterize OMT-like cDNA clones from Arabidopsis thaliana and Serratula tinctoria , and carry out a phylogenomic analysis of well characterized OMTs to develop a framework phylogenetic tree for predicting putative function and substrate specificity of newly identified gene sequences. Two OMT-like cDNA clones of A. thaliana obtained from TAIR (The Arabidopsis Information Resources) were heterologously expressed for molecular and biochemical characterization. The recombinant proteins did not show enzymatic activity with the substrates tested suggesting that these two putative OMTs genes could be pseudogenes. S. tinctoria accumulates small amounts of 3-methylquercetin as an intermediate for the synthesis of 3,3'-dimethyl derivative.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Thesis (Masters)
Authors:Lam, Kevin Chee Loong
Pagination:xi, 131 leaves : ill. ; 29 cm.
Institution:Concordia University
Degree Name:M. Sc.
Program:Biology
Date:2005
Thesis Supervisor(s):Dayanandan, Selvadurai and Ibrahim, Ragaï
Identification Number:LE 3 C66B56M 2005 L36
ID Code:8478
Deposited By: Concordia University Library
Deposited On:18 Aug 2011 18:26
Last Modified:13 Jul 2020 20:04
Related URLs:
All items in Spectrum are protected by copyright, with all rights reserved. The use of items is governed by Spectrum's terms of access.

Repository Staff Only: item control page

Downloads per month over past year

Research related to the current document (at the CORE website)
- Research related to the current document (at the CORE website)
Back to top Back to top