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Cloning, functional expression and characterization of three Phanerochaete chrysosporium endo-1,4-beta-xylanases

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Cloning, functional expression and characterization of three Phanerochaete chrysosporium endo-1,4-beta-xylanases

Decelle, Barbara (2006) Cloning, functional expression and characterization of three Phanerochaete chrysosporium endo-1,4-beta-xylanases. Masters thesis, Concordia University.

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Abstract

Three Phanerochaete chrysosporium endo-1,4-β-xylanase genes were cloned and expressed in Aspergillus niger . Two of these genes, xynA and xynC , encode family 10 glycoside hydrolases, while the third, xynB , codes for a family 11 glycoside hydrolase. All three xylanases possess a type I carbohydrate-binding domain connected to the catalytic domain by a linker region. The three xylanases were purified to homogeneity by weak anion or Avicell column chromatography and subsequently characterized. The XynA, XynB and XynC enzymes have molecular masses of 52, 30 and 50 kDa, respectively. Optimal activity was obtained at pH 4.5 and 70 degrees C with the family 10 xylanases and pH 4.5 and 60 degrees C with the family 11 xylanase. The measured Km when using birchwood xylan as the substrate was 3.71 +/- 0.69 mg/ml for XynA and XynC and was 9.96 +/- 1.45 mg/ml for XynB. Substrate specificity studies and the products released during the degradation of birchwood xylan suggest differences in catalytic properties between the two family 10 xylanases and the family 11 xylanase.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Thesis (Masters)
Authors:Decelle, Barbara
Pagination:xiii, 116 leaves : ill. ; 29 cm.
Institution:Concordia University
Degree Name:M. Sc.
Program:Biology
Date:2006
Thesis Supervisor(s):Storms, Reginald
ID Code:8745
Deposited By:Concordia University Libraries
Deposited On:18 Aug 2011 14:34
Last Modified:18 Aug 2011 15:13
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