Login | Register

The Interaction of the Stress Regulated Wheat (Triticum aestivum) Proteins, ESI2 and RAN1, and the Localization of Ipp-1 and Pr-1

Title:

The Interaction of the Stress Regulated Wheat (Triticum aestivum) Proteins, ESI2 and RAN1, and the Localization of Ipp-1 and Pr-1

Carson, Robert Spencer (2012) The Interaction of the Stress Regulated Wheat (Triticum aestivum) Proteins, ESI2 and RAN1, and the Localization of Ipp-1 and Pr-1. Masters thesis, Concordia University.

[thumbnail of Carson_MSc_S2012.pdf]
Preview
Text (application/pdf)
Carson_MSc_S2012.pdf - Accepted Version
419kB

Abstract

The Early Salt-Stress Induced 2 protein, ESI2, from wheat (Triticum aestivum) was identified as an interacting protein with the GTP-binding protein RAN1. Localization of both ESI2, and RAN1, has been performed using fluorescent protein fusions, transiently expressed in Nicotiana belthamiana, and observed using confocal fluorescent microscopy. The proteins fused to independent fluorescent partners localized to separate cellular compartments, with RAN1 primarily localized to the nucleus. However, the interaction of the two proteins detected by bimolecular fluorescent complementation, was found to be localized to the tonoplast. This interaction suggests a potential mechanism for the role of ESI2 in the sequestration of RAN1 and the regulation of cell division in response to stress. Two additional proteins, expressed in wheat during stress response and shown previously, by Y2H assays to bind with ESI2, where also localized. To further explore the effects of RAN1 on cell division in plants, a homozygous mutant for the Arabidopsis Ran1 was identified and a change in root growth shortly after germination was noted.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Thesis (Masters)
Authors:Carson, Robert Spencer
Institution:Concordia University
Degree Name:M. Sc.
Program:Biology
Date:March 2012
Thesis Supervisor(s):Gulicks, Patrick
Keywords:Wheat, Stress, Abiotic Stress, ESI2, Early Salt Stressed 2, RAN, Ipp1, Inorganic Pyrophosphatase, Pr-1, Pathogen-Related, Salt-Stress, Cold-Stress, Drought-Stress, Protein Interaction, Protein Localization, Homeologe, Homeologous.
ID Code:973905
Deposited By: ROBERT SPENCER CARSON
Deposited On:20 Jun 2012 15:03
Last Modified:18 Jan 2018 17:37

References:

Adie B, Perez-Perez J, Perez-Perez M, Godoy M, Sanchez-Serrano J, Schmelz E, Solano R, (2007) ABA Is an Essential Signal for Plant Resistance to Pathogens Affecting JA Biosynthesis and the Activation of Defences in Arabidopsis. The Plant Cell: May, Vol. 19, 1665-1681

Agarie S, Shimoda T, Shimuzu Y, Baumann K, Sunagawa H, Kondo A, Ueno O, Nakahara T, Nose A, Cushman J C, (2007) Salt tolerance, salt accumulation, and ionic homeostasis in an epidermal bladder-cell-ess mutant of the common ice plant Mesembryanthemum crystallinum. Journal of Experimental Botany. Vol. 58. p. 1957-1967

Aguirrezabal L, Bouchier-Combaud S, Radziejwoski A, Dauzat M, Cookson S J, Granier C, (2006) Plasticity to soil water deficit in Arabidopsis thaliana: dissection of leaf development into underlying growth dynamic and cellular variables reveals invisible phenotypes, Vol. 29, p. 2216-2227

Battaglia M, Olvera-Carrillo Y, Garciarrubio A, Campos F, Covarrubias A A, (2008) The enigmatic LEA Proteins and Other Hydrophilins. Plant Physiology, Sep. Vol. 148 p. 6-24

Bartels D, and Sunkar R, (2005) Drought and Salt Tolerance in Plants. Critical Reviews in Plant Sciences. Vol 24, p. 23-58

Belhumeur P, Lee A, Tam R, DiPaolo T, Fortin N, Clark M W, (1993) GSP1 and GSP2, genetic suppressors of the prp20-1 mutant in Saccharomyces cerevisiae: GTP-binding proteins involved in the maintenance of nuclear organization. Molecular and Cellular Biology. Apr. Vol. 13. No. 4. p. 2152-2161

Benson D A, Karsch-Mizrachi I, Lipman D J, Ostell J, Wheeler D L, (2005) GenBank, Nucleic Acids Research, Vol. 33. Database issue

Binzel M L, Hess F D, Bressan R A, Hasegawa P M, (1988) Intracellular compartmentation of ions in salt adapted tobacco cells. Plant Physiology Vol. 86. p. 607-614

Bischoff F R, Ponstingl H, (1991) Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. Proceedings of the National Academy of Sciences Dec. Vol. 88. p. 10830-10834

Bischoff F R, Klebe C, Kretschmer J, Wittinghofer A, Ponstingl H, (1994) RanGAP1 induces GTPase activity of nuclear Ras-related Ran. Proceedings of the National Academy of Sciences Vol. 91. p. 2587-2591

Boudart G, Jamet E, Rossignol M, Lafitte C, Borderies G, Jauneau A, Esquerre-Tugaye M T, Pont-Lezica R, (2005) Cell wall proteins in apoplastic fluids of Arabidopsis thaliana rosettes: identification by mass spectrometry and bioinformatics. Proteomics. Vol 5. p. 212-221

Bruice P Y, (1998) Organic Chemistry second edition, Prentice-Hall, Inc, Upper Saddle River, New Jersey ISBN 0-13-841925-6 p. 1061-1105

Bruneel A, Labas V, Mailloux A, Sharma S, royer N, Vinh J, Pernet P, Vaubourdolle M, Baudin B, (2005) Proteomics of human umbilical vein endothelial cells applied to etoposide-induced apoptosis. Proteomics 5, p 3876-3884

Buell C R, Somerville S C, (1997) Use of Arabidopsis recombinant inbred lines reveals a monogenic and a novel digenic resistance mechanism to Xanthomanas campestris pv campestris.

Clarke P R, Klebe C, Wittinghofer A, Karsenti E, (1995) Regulation of Cdc2/cyclin B activation by Ran, a Ras-related GTPase. Journal of Cell Science. Vol.108 p. 1217-1225

Claugh S J, Bent A F, (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. The Plant Journal Vol. 16 issue 6 p 735-743

Chen G, Gharib T G, Huang C C, Thomas D G, Shedden K A, Taylor J M G, Kardia S L R, Misek D E, Giordano T J, Iannettoni M D, Orringer M B, Hanash S M, Beer D G, (2002) Proteomic Analysis of Lung Adenocarcinoma: Identification of a Highly Expressed set of Proteins in Tumors. Clinicar Cancer Research. Vol. 8. p. 2298-2305

Curry J, Morris C F, Walker-Simmons M K, (1991) Seqeunce analysis of a cDNA encoding a group 3 LEA mRNA inducible by ABA or dehydration stress in wheat. Plant Molecular Biology. Vol. 16. p. 1073-1076

Cushman I, Stenoien D, Moore M S (2004) The Dynamic association of RCC1 with Chromatin Is Modulated by Ran-dependent Nuclear Transport. Molecular Biology of the Cell. Vol. 15. p. 245-255

Cushman J C, Bohnert H J, (1997) Molecular biology of CAM. Plant physiology. Vol 113. p. 667-676

Dure L (1993) A repeating 11-mer amino acid motif and plant desiccation. The Plant Journal Vol. 3 issue 3, p. 363-369

Ekman D, Light S, Bjorklund A K, Elofsson A, (2006) What properties characterize the hub proteins of the protein-protein interaction network of Saccharomyces cerevisiae?

Eisen M, Brown P O, (1999) DNA Arrays for Analysis of Gene Expression. Methods of Enzymology, Vol. 17. p. 217-218

English J J, Mueller E, Baulcombe C, (1996) Suppression of Virus Accumulation in Transgenic Plants Exhibiting Silencing of Nuclear Genes. The Plant Cell, Vol. 8. p. 179-188

Feig L A, Cooper G M, (1988) Inhibition of NIH 3T3 Cell Proliveration by a Mutant ras Protein with Preferential Affinity for GDP. Molecular and Cellular Biology Vol. 8. p. 3235-3243

Fricke W, Akhiyarova G, Wei W, Alexandersson E, Miller A, Kjellbom P O, Richardson A, Wojciechowski T, Schreiber L, Veselov D, Kudoyarova G, Volkov V, (2006) The short-term growth response to salt of the developing barley leaf. Journal of Experimental botany, Vol. 57 p. 1079-1095

Fu D, Szucs P, Yan L, Helquera M, Skinner J S, Von Zitzewitz J, Hayes P M, Dubcovsky J, (2005) Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat. Molecular Genetics and Genomics. Nov, Vol 274, issue 4, p. 442-443

Galvez A F, Gulick P J, Dvorak J, (1993) Characterization of the Early Stages of Genetic Salt-Stress Responses in Salt-Tolerant Lophopyrum elongatum, Salt-Sensitive Wheat, and Their Amphiploid. Plant Physiol Vol. 103 p. 257-265

Garay-Arroyo A, Colmenero-Flores J M, Garciarrubio A, Covarrubias A A, (2000) Hightly Hydrophilic Proteins in Prokaryotes and Eukaryotes Are Common during conditions of Water Deficit. Journal of Biological Chemistry. Vol. 275. p. 5668-5674

Ghosh K, Maitra S, Goswami L, Roy D, Pada K, Majumder A L, (2006) An insight into the molecular basis of salt tolerance of L-myo-Inositol 1-P Synthase (PcINO1) from Porteresia coarctiata (Roxb.) Tateoka, a Halophytic Wild Rice. Plant Physiology, Vol. 140. p. 1279-1296

Gorham J, Wyn Jones R G, Bristol A, (1990) Partial characterization of the trait for enhanced K+-Na+ discrimination in the D genome of wheat. Planta Vol. 180 p. 590-597

Gorlich D, Kutay U, (1999) Transport Between the Cell Nucleus and the Cytoplasm. Cell Developmental Biology, Vol. 15 p 607-660

Gulick P J, Drouin S, Yu Z, Danyluk J, Poisson G, Monroy A, Sarhan F, (2005) transcriptome comparison of winter and spring wheat responding to low temperature. Genome, Vol. 48, p. 913-923

Gulick P J, Dvorak J, (1992) Coordinate Gene Response to Salt Stress in Lophopyrum elongatum. Vol. 100. p. 1384-1388

Halpin D, Kalab P, Wang J, Weis K, Heald R, (2011) Mitotic Spindle Assembly around RCC1-Coated Beads in Xenopus Egg Extracts. Vol 9. e1001225

Hajjar R, Hodgkin R, (2007) The use of wild relatives in crop improvment: a survey of development over the last 20 years. Springer Science and Buisiness Media. Feb 9.

Hasegawa P M, Bressan R A, Zhu J K, Bohnert H J, (2000) Plant cellular and Molecular Responses to High Salinity. Annu Rev Plant Physiol Plant Molecular Biology, Vol. 51. p. 463-499

Hong B, Uknes, S J, Ho T D, (1988) Cloning and characterization of a cDNA encoding a mRNA rapidly-induced by ABA in barley aleurone layers. Plant Molecular Biology. Vol. 11. p 495-506

Hu C D, Chinenov Y, Kerppola T K, (2002) Visualization of interactions among bZIP and Rel Family proteins in living cells using bimolecular fluorescence. Molecular Cell Vol. 9. p 789-798

Hughes M, Zhang C, Avis J M, Hutchison C J, Clark P R, (1998) The role of Ran GTPase in nuclear assembly and DNA replication: characterisation of the effects of Ran mutants. Journal of Cell Science. Vol. 111. p. 3017-3026

Inze D, (2005) Creen light for the cell cycle. The EMBO Journal. Vol. 24. p. 657-662

Izaurralde E, Kutay U, Von Kobbe C, Mattaj I W, Gorlich D. (1997) The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus. The EMBO Journal Vol. 16. No. 21. p 6535-6547

du Jardin P, Rojas-Beltran J, Gebhardt C, Brasseur R (1995) Molecular Cloning and Characterization of a soluble Inorganic Pyrophosphatase in Potato. Phland physiology Vol. 109 p. 853-860

Kim J C, Lee S H, Cheong Y H, Yoo C M, Lee S I, Chun H J, Yun D J, Hong J C, Lee S Y, Lim C O, Cho M J, (2001) A novel cold-inducible zinc finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants. The Plant Journal Vo. 25. p. 247-259

Kleb C, Prinz H, Wittinghofer A, Goody R S, (1995) The Kinetic Mechanism of Ran-Nucleotide Exchange Catalyzed by RCC1. Biochemistry. Vol. 34. p. 12543-12552

Kornbluth S, Dasso M, Newport J, (1994) Evidence for a Dual Role for TC4 Protein in Regulating Nuclear Structure and Cell Cycle Progression. The Journal of Cell Biology, Vol. 125. p. 705-719

Kreps J A, Wu Y, Chang H, Zhu T, Wang X, Harper J, (2002) Transcriptome Changes for Arabidopsis in Response to Salt, Osmotic, and Cold Stress. Dec. Vol 130 no. 4 p. 2129-2141

Lahti R, Kolakowski L F Jr, Heinonen J, Vihinen M, Pohjanoksa K, Cooperman B S, (1990) Conservation of functional residues between yeast and E. coli inorganic pyrophosphatases. Biochemical et Biophysica Acta (BBA) – Protein Structure and Molecular Enzymology, Vol 1038. p. 338-345

Lane A, Jarvis A, (2007) Changes in Climate will modify the Geography of Crop Suitability: Agricultural Biodiversity can help with Adapation. SAT eJournal: Dec, Vol 4, Issue 1

Laudencia-Chingcuanco D, Ganeshan S, You F, Fowler B, Chibbar R, Anderson O, (2011) Genome-wide gene expression analysis supports a developmental model of low temperature tolerance gene regulation in wheat (Triticum aestivum L.) BMC Genomics Vol. 12 issue 299

Lin C, Guo W W, Everson E, Thomashow M F. (1990) Cold Acclimation in Arabidopsis and Wheat. Plant Physiology Vol. 94 p. 1078-1083

Lodish H, Berk A, Krieger, Kaiser, Scott, Bretsher, Ploegh, Matsuaira (2008) Molecular Cell Biology. W. H. Freeman and Company. ISBN 0716776014

Lopez-Marques R L, Perez-Castineira J R, Losada M, Serrano A, (2004) Differential regulation of soluble and membrane bound inorganic pyrophosphatases in the photosynthetic bacterium Rhodospirillum rubrum provides insights into pyrophosphate-based stress bioenergetics. Journal of Bacteriology. Vol. 186. p. 5418-5426

Lu S, Friesen T L, Faris J D, (2011) Molecular characterization and genomic mapping of the pathogenesis-related protein 1 (PR-1) gene family in hexaploid wheat (Triticum aestivum L.). Molecular Genetics and Genomics. Vol. 285. p. 485-503

Mardi M, Naghavi M R, Pirseyedi S M, Alamooti M K, Monfared S R, Ahkami A H, Omidbakhsh M A, Alavi N S, Shanjani P S, Katsiotis A, (2011) Comparative Assesment of SSAP, AFLP and SSR Markers for Evaluation of Genetic Diversity of Durum Wheat. Journal of Agricultural Science and Technology: Vol. 13, p. 905-920

Marchler-Bauer A, Anderson J B, Chitsaz F, Derbyshire M K, DeWeese-Scott C, Fong J H, Geer L Y, Geer R C, Gonzales N R, Gwadz M, He S, Hurwitz D I, Jackson J D, Ke Z, Lanczycki C J, Liebert C A, Liu C, Lu F, Lu S, Garchler G H, Mullokandov M, Song J S, Tasneem A, Thanki N, Yamashita R A, Zhang D, Zhang N, Bryant S H, (2009) CDD: specific functional annotation with the Conserved Domain Database. Nucleic Acids Research. Vol 37 D205-D210

Marchler-Bauer A, Anderson J B, Chitsaz F, Derbyshire M K, DeWeese-Scott C, Fong J H, Geer L Y, Geer R C, Gonzales N R, Gwadz M, He S, Hurwitz D I, Jackson J D, Ke Z, Lanczycki C J, Liebert C A, Liu C, Lu F, Lu S, Marchler G H, Mullokandov M, Song J S, Tasneem A, Thanki N, Yamashita R A, Zhang D, Zhang N, Bryant S H, (2011) CDD: a Conserved Domain Database for the Functional annotation of proteins. Nucleic Acids Res. 39 (D) 225-9

Merkle T, Haizel T, Matsumoto T, Harter K, Dallmann G, Nagy F (1994) Phenotype of the fission yeast cell cycle regulatory mutant pim1-46 is suppressed by a tobacco cDNA encoding a small, Ran-like GTP-binding protein. Plant Journal, Vol6. p. 555-565

Meyerowitz E M, (2001) Prehistory and History of Arabidopsis Research. Plant Physiology Jan. Vol. 125. p. 15-19

Monroy A F, Dryanova A, Malette B, Oren D H, Farajalla M R, Liu W, Danyluk J, Ubayasena L W C, Kane K, Scoles G J, (2007) Regulatory gene candidates and gene expression analysis of cold acclimation in winter and spring wheat. Plant Molecular biology July Vol. 64. issue 4 p. 409-423

Morillon R, Lienard D, Chrispeels M J, Lassalles J P, (2001) Rapid movements of plants organs requre solute-water cotransporters or contractile proteins. Plant Physiology. Vol 127 p. 720-723

Munns R, (2002) Comparative physiology of salt and water stress. Plant, Cell and Environment Vol. 25. p. 239-250

Musacchio A, Hardwick K G (2002) The spindle check-point: Structural insights into dynamic signalling. National Revew in Molecular Cell Biology. Vol. 3. p 731-741

Nakashima K and Yamaguchi-Shinozaki K (2006) Regulations involved in osmotic stress-responsive and cold stress-responsive gene expression in plants. Physiologia Plantarum Vol. 126 p. 62-71

Nelson D, Cox M M, (2000) Lehninger Principles of biochemistry, 3rd ed. New Yourk, worth Publishers p. 937 ISBN 1-57259-153-6

Pardo J M, (2010) Biotechnology of water and salinity stress tolerance. Current Opinions in Biotechnology. Vol. 21 p. 185-196

Rabbani M A, Maruyama K, Abe H, Khan M A, Katsura K, Ito Y, Yoshiwara K, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) Monitoring Expression Profiles of Rice enes under Cold, Drough, and High-Salinity Stresses and Abscisic Acid Application Using cDNA Microarray and RNA Gel-Blot Analysis. Plant Physiology Vol 133. p. 1755-1767

Renault L, Nassar N, Vetter I, Becker J, Klebe C, Roth M, Wittinghofer A, (1998) the 1.7 A crystal structure of the regulator of chromosome condentation (RCC1) reveals a seven-bladed propeller. Nature March, Vol 392 issue 6671 p. 97-101

Reyes J L, Rodrigo M J, Colmenero-Flores J M, Gil J V, Garay-Arroyo A, Campos F, Salamini F, Bartels D, Covarrubias A A, (2005) Hydrophilins from distant organisms can protect enzymatic activities from water limitation effects in vitro. Plant, Cell and Environment Vol. 28, p 709-718

Ribbeck K, Lipowsky G, Kent H M, Stewart M, Gorlich D, (1998) NTF2 mediates nuclear import of Ran. The EMBO Journal, Vol. 22 p 6587-6598

Ridaha Farajalla M, Gulick P J, (2007) The α-tubulin gene family in wheat (Triticum aestivum L.) and differential gene expression during cold acclimation. Genome Vol. 50 p502-510

Sazer S, Dasso M, (2000) The Ran Decathlon: Multiple roles of Ran. Journal of Cell Science. Vol 113. p. 1111-1118

Schena M, Shalon D, Davis R W, Brown P O, (1995) Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray. Science, Vol. 270 no. 5235 p. 467-470

Seki M, Narusaka M, Abe H, Kasuga M, Yamaguchi-Shinozaki K, Carninci P, Hayashizaki Y, Shinozaki K, (2001) Monitoring the Expression Pattern of 1300 Arabidopsis Genes under Drought and Cold Stresses by using a Full-Length cDNA Microarray. The Plant Cell, Vol. 13. p. 61-72

Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T, Satou M, Akiyama K, Taji T, Yamaguchi-Shinozaki K, Carninci P, Kawai J, Hayashizaki Y, Shinozaki K, (2002) Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. The Plant Journal Vol 31, p. 279-292

Shaner N C, Campbell R E, Steinbach P A, Giepmans B N G, Palmer A E, Tsien R Y, (2004) Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. Red fluorescent protein. Nature Biotechnology. Vol. 22. No. 12 p. 1567-1572

Shi X, Basran J, Seward H E, Childs W, Bagshaw C R, Boxer S G, (2007) Anomalous Negative Fluorescence Anisotropy in Yellow Fluorescent Protein (YFP 10C): Quantitative Analysis of Fret in YFP Dimers. Biochemistry Dec. Vol 46 no. 50. p 14403-14417

Shinozaki K, Yamaguchi-Shinozaki K, (2006) Gene networks involved in drought stress response and tolerance. Journ
All items in Spectrum are protected by copyright, with all rights reserved. The use of items is governed by Spectrum's terms of access.

Repository Staff Only: item control page

Research related to the current document (at the CORE website)
- Research related to the current document (at the CORE website)
Back to top Back to top