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Phosphatidylinositol-specific phospholipase C interaction with G-proteins in relation to the environmental stress response in wheat (Triticum aestivum)

Title:

Phosphatidylinositol-specific phospholipase C interaction with G-proteins in relation to the environmental stress response in wheat (Triticum aestivum)

Khalil, Hala (2009) Phosphatidylinositol-specific phospholipase C interaction with G-proteins in relation to the environmental stress response in wheat (Triticum aestivum). Masters thesis, Concordia University.

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Abstract

Tolerance to environmental stress in plants is controlled by several internal factors and environmental signals. In this study, we have focused on phosphatidylinositol-specific phospholipase C (TaPI-PLC) and phosphoglycerol-specific phospholipase C (TaPG-PLC) in wheat, Triticum aestivum . Three homologs of TaPG-PLCs were identified as cDNA with high sequence similarity to rice and Arabidopsis PG-PLC1. TaPG-PLC3 was mapped on the distal part of the long arm of chromosome 5A, whereas TaPG-PLC1 and TaPG-PLC2 like genes appear to be duplicated in two of hexploid wheat 3 three genomes. A TaPG-PLC1::Green fluorescent protein (GFP)-fusion was localized on endoplasmic reticulum (ER), golgi and plasma membrane (PM). Important protein-protein interactions were found for wheat PI-PLCs. The PI-PLCI::GFP fusion was localized on ER-and PM. In vivo interactions between TaPI-PLC1 and two classes of G-proteins, the heterotrimeric G protein subunit TaGa and a non-canonicat G-protein J822, were detected using Bimolecular Fluorescent Complementation (BiFC). The interactions were detected in both the ER and PM. In contrast, TaPI-PLC2::GFP fusion was localized only on PM and was not found to interact with TaGÜ or J822 . Since both TaPI-PLC 1 and J822 are up-regulated by cold stress and it is possible that they play an important role in signaling during cold acclimation

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Thesis (Masters)
Authors:Khalil, Hala
Pagination:xii, 89 leaves : ill. (chiefly col.) ; 29 cm.
Institution:Concordia University
Degree Name:M. Sc.
Program:Biology
Date:2009
Thesis Supervisor(s):Gulick, P
ID Code:976469
Deposited By: Concordia University Library
Deposited On:22 Jan 2013 16:26
Last Modified:18 Jan 2018 17:42
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