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Influence of Electric Fields and Conductivity on Pollen Tube Growth assessed via Electrical Lab-on-Chip

Title:

Influence of Electric Fields and Conductivity on Pollen Tube Growth assessed via Electrical Lab-on-Chip

Agudelo, Carlos, Packirisamy, Muthukumaran ORCID: https://orcid.org/0000-0002-1769-6986 and Geitmann, Anja (2016) Influence of Electric Fields and Conductivity on Pollen Tube Growth assessed via Electrical Lab-on-Chip. Scientific Reports, 6 (19812). pp. 1-15. ISSN 2045-2322

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Official URL: http://dx.doi.org/10.1038/srep19812

Abstract

Pollen tubes are polarly growing plant cells that are able to rapidly respond to a combination of chemical, mechanical, and electrical cues. This behavioural feature allows them to invade the flower pistil and deliver the sperm cells in highly targeted manner to receptive ovules in order to accomplish fertilization. How signals are perceived and processed in the pollen tube is still poorly understood. Evidence for electrical guidance in particular is vague and highly contradictory. To generate reproducible experimental conditions for the investigation of the effect of electric fields on pollen tube growth we developed an Electrical Lab-on-Chip (ELoC). Pollen from the species Camellia displayed differential sensitivity to electric fields depending on whether the entire cell or only its growing tip was exposed. The response to DC fields was dramatically higher than that to AC fields of the same strength. However, AC fields were found to restore and even promote pollen growth. Surprisingly, the pollen tube response correlated with the conductivity of the growth medium under different AC frequencies—consistent with the notion that the effect of the field on pollen tube growth may be mediated via its effect on the motion of ions.

Divisions:Concordia University > Gina Cody School of Engineering and Computer Science > Mechanical, Industrial and Aerospace Engineering
Item Type:Article
Refereed:Yes
Authors:Agudelo, Carlos and Packirisamy, Muthukumaran and Geitmann, Anja
Journal or Publication:Scientific Reports
Date:25 January 2016
Funders:
  • Fonds Québécois de la Recherche sur la Nature et les Technologies (FQRNT)
Digital Object Identifier (DOI):10.1038/srep19812
Keywords:Cell growth; Tropism
ID Code:983234
Deposited By: DANIELLE DENNIE
Deposited On:24 Nov 2017 21:40
Last Modified:18 Jan 2018 17:56

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