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Nano-Integrated Suspended Polymeric Microfluidics (SPMF) Platform for Ultra-Sensitive Bio-Molecular Recognition of Bovine Growth Hormones

Title:

Nano-Integrated Suspended Polymeric Microfluidics (SPMF) Platform for Ultra-Sensitive Bio-Molecular Recognition of Bovine Growth Hormones

SadAbadi, Hamid and Packirisamy, Muthukumaran ORCID: https://orcid.org/0000-0002-1769-6986 (2017) Nano-Integrated Suspended Polymeric Microfluidics (SPMF) Platform for Ultra-Sensitive Bio-Molecular Recognition of Bovine Growth Hormones. Scientific Reports, 7 (1). ISSN 2045-2322

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Official URL: http://dx.doi.org/10.1038/s41598-017-11300-2

Abstract

The development of sensitive platforms for the detection of biomolecules recognition is an extremely important problem in clinical diagnostics. In microcantilever (MC) transducers, surface-stress is induced upon bimolecular interaction which is translated into MC deflection. This paper presents a cost-effective and ultra-sensitive MC-based biosensing platform. To address these goals, the need for costly high-resolution read-out system has been eliminated by reducing the cantilever compliance through developing a polymer-based cantilever. Furthermore a microfluidic system has been integrated with the MC in order to enhance sensitivity and response time and to reduce analytes consumption. Gold nanoparticles (AuNPs) are synthesized on the surface of suspended microfluidics as the selective layer for biomolecule immobilization. The biosensing results show significant improvement in the sensitivity of the proposed platform compared with available silicon MC biosensor. A detection limit of 2 ng/ml (100pM) is obtained for the detection of bovine growth hormones. The results validated successful application of suspended polymeric microfluidics (SPMF) as the next generation of biosensing platforms which could enable femtomolar (fM) biomolecular recognition detection.

Divisions:Concordia University > Gina Cody School of Engineering and Computer Science > Mechanical, Industrial and Aerospace Engineering
Item Type:Article
Refereed:Yes
Authors:SadAbadi, Hamid and Packirisamy, Muthukumaran
Journal or Publication:Scientific Reports
Date:2017
Funders:
  • Concordia Open Access Author Fund
  • NSERC
  • Concordia Research Chair grants
Digital Object Identifier (DOI):10.1038/s41598-017-11300-2
ID Code:983981
Deposited By: KRISTA ALEXANDER
Deposited On:26 Jun 2018 15:16
Last Modified:26 Jun 2018 15:16

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