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Detoxification of host plant phenolic aglycones by the spruce budworm

Title:

Detoxification of host plant phenolic aglycones by the spruce budworm

Donkor, Dominic, Mirzahosseini, Zahra, Bede, Jacquie, Bauce, Eric and Despland, Emma (2018) Detoxification of host plant phenolic aglycones by the spruce budworm. [Dataset]

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Spreadsheet (Data for PLOS One MS on spruce budworm detoxification) (text/plain)
bradford.csv - Data
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Spreadsheet (Data for PLOS One MS on spruce budworm detoxification) (text/plain)
budworm_mass.csv - Data
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Spreadsheet (Data for PLOS One MS on spruce budworm detoxification) (text/plain)
GSTneutral.csv - Data
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Image (Data for PLOS One MS on spruce budworm detoxification) (image/jpeg)
HPLC acetophenones at pH7.JPG - Data
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Image (Data for PLOS One MS on spruce budworm detoxification) (image/jpeg)
HPLC acetophenones at pH9.JPG - Data
Available under License Spectrum Terms of Access.
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Image (Data for PLOS One MS on spruce budworm detoxification) (image/jpeg)
HPLC aglycons in frass.JPG - Data
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Image (Data for PLOS One MS on spruce budworm detoxification) (image/jpeg)
HPLC dimers in frass.JPG - Data
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Image (Data for PLOS One MS on spruce budworm detoxification) (image/jpeg)
HPLC glycosides in frass.JPG - Data
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Image (Data for PLOS One MS on spruce budworm detoxification) (image/jpeg)
HPLC GSH conjugates.JPG - Data
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Abstract

This study examines the post-ingestive fate of two host-plant derived small-molecule phenolics (the acetophenones piceol and pungenol) that have previously been shown to be toxic to the outbreaking forest pest, spruce budworm (Choristoneura fumiferana). We test first whether these compounds are transformed during passage through the midgut, and second whether the budworm upregulates activity of the detoxification enzyme glutathione-s-transferase (GST) in response to feeding on these compounds. Insects were reared on either foliage or artificial diet to the fourth instar, when they were transferred individually to one of two treatment diets, either control or phenolic-laced, for approximately 10 days, after which midguts were dissected out and used for Bradford soluble protein and GST enzyme activity analysis. Frass was collected and subjected to HPLC-DAD-MS. HPLC showed that the acetophenones do not autoxidize under midgut pH conditions, but that glucose- and glutathione- conjugates are present in the frass of insects fed the phenolic-laced diet. GST enzyme activity increases in insects fed the phenolic-laced diet, in both neutral pH and alkaline assays. These data show that the spruce budwom exhibits counter-adaptations to plant phenolics similar to those seen in angiosperm feeders, upregulating an important detoxifying enzyme (GST) and partially conjugating these acetophenones prior to elimination, but that these counter-measures are not totally effective at mitigating toxic effects of the ingested compounds in the context of our artifical-diet based laboratory experiment.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Dataset
Authors:Donkor, Dominic and Mirzahosseini, Zahra and Bede, Jacquie and Bauce, Eric and Despland, Emma
Date:12 November 2018
ID Code:984684
Deposited By: EMMA DESPLAND
Deposited On:02 May 2019 19:51
Last Modified:02 May 2019 20:14
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