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Characterization of the Esi3/RCI2/PMP3 gene family in the Triticeae

Title:

Characterization of the Esi3/RCI2/PMP3 gene family in the Triticeae

Brunetti, Sabrina C., Arseneault, Michelle K. M. and Gulick, Patrick J. (2018) Characterization of the Esi3/RCI2/PMP3 gene family in the Triticeae. BMC Genomics, 19 (1). ISSN 1471-2164

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Official URL: http://dx.doi.org/10.1186/s12864-018-5311-8

Abstract

Background
Members of the Early Salt Induced 3 (Esi3/RCI2/PMP3) gene family in plants have been shown to be induced in response to both biotic and abiotic stresses and to enhance stress tolerance in both transgenic plants and Saccharomyces cerevisiae. Esi3 was first identified as a salt stress induced gene in the salt tolerant wild wheat grass, Lophopyrum elongatum, and subsequently homologous genes in many other species were found to be members of the gene family. These include Arabidopsis thaliana and Oryza sativa where they are referred to as Rare Cold Inducible 2 (RCI2), and Zea mays where they are referred to as Plasma Membrane Protein 3 (PMP3). This study characterizes the Esi3 family members in Triticum aestivum and explores the tissue specific expression patterns of the gene family members as well as their response to a variety of environmental stresses.

Results
The Esi3 gene family was found to have a total of 29 family members comprised of ten paralogous groups in the hexaploid T. aestivum. Each paralogous group contains three homeologous copies, one in each of the A, B and D genomes with the exception of Esi3–2 which is missing the B copy. The genes of the Esi3 gene family were also identified in four other monocot species, Aegilops tauschii, Hordeum vulgare, Secale cereale and Sorghum bicolor, and were confirmed or corrected for Brachypodium distachyon, Oryza sativa and Zea mays, as well as the dicot Arabidopsis thaliana. Gene expression of the Esi3s was analyzed using tissue-specific, abiotic and biotic stress RNA-Seq 454 sequence libraries and Affymetrix microarray data for T. aestivum.

Conclusions
Members of nearly all paralogous groups of the Esi3 genes in T. aestivum have altered gene expression in response to abiotic or biotic stress conditions. In addition, there are modest differences in gene expression among homeologous members of the gene family. This suggests that the Esi3 gene family plays an important role in the plants response to the stresses presented in this study. The Esi3–9 in T. aestivum has a unique N terminal extension placing it into Group III, a new group for the Esi3/RCI2/PMP3 gene family.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Article
Refereed:Yes
Authors:Brunetti, Sabrina C. and Arseneault, Michelle K. M. and Gulick, Patrick J.
Journal or Publication:BMC Genomics
Date:2018
Funders:
  • Concordia Open Access Author Fund
  • Natural Science and Engineering Research Council of Canada
Digital Object Identifier (DOI):10.1186/s12864-018-5311-8
Keywords:Early salt induced gene family, Esi3, Tissue-specific expression, Rare Cold Inducible 2, RCI2, Plasma Membrane Protein 3, PMP3, RNA-seq
ID Code:986025
Deposited By: KRISTA ALEXANDER
Deposited On:22 Oct 2019 18:36
Last Modified:22 Oct 2019 18:36

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