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Characterization of Phase I and Glucuronide Phase II Metabolites of 17 Mycotoxins Using Liquid Chromatography—High-Resolution Mass Spectrometry

Title:

Characterization of Phase I and Glucuronide Phase II Metabolites of 17 Mycotoxins Using Liquid Chromatography—High-Resolution Mass Spectrometry

Slobodchikova, Irina, Sivakumar, Reajean, Rahman, Md Samiur and Vuckovic, Dajana ORCID: https://orcid.org/0000-0002-7764-8492 (2019) Characterization of Phase I and Glucuronide Phase II Metabolites of 17 Mycotoxins Using Liquid Chromatography—High-Resolution Mass Spectrometry. Toxins, 11 (8). p. 433. ISSN 2072-6651

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Official URL: http://dx.doi.org/10.3390/toxins11080433

Abstract

Routine mycotoxin biomonitoring methods do not include many mycotoxin phase I and phase II metabolites, which may significantly underestimate mycotoxin exposure especially for heavily metabolized mycotoxins. Additional research efforts are also needed to measure metabolites in vivo after exposure and to establish which mycotoxin metabolites should be prioritized for the inclusion during large-scale biomonitoring efforts. The objective of this study was to perform human in vitro microsomal incubations of 17 mycotoxins and systematically characterize all resulting metabolites using liquid chromatography–high-resolution mass spectrometry (LC-HRMS). The results obtained were then used to build a comprehensive LC-MS library and expand a validated 17-mycotoxin method for exposure monitoring to screening of additional 188 metabolites, including 100 metabolites reported for the first time. The final method represents one of the most comprehensive LC-HRMS methods for mycotoxin biomonitoring or metabolism/fate studies.

Divisions:Concordia University > Faculty of Arts and Science > Chemistry and Biochemistry
Item Type:Article
Refereed:Yes
Authors:Slobodchikova, Irina and Sivakumar, Reajean and Rahman, Md Samiur and Vuckovic, Dajana
Journal or Publication:Toxins
Date:2019
Funders:
  • Concordia Open Access Author Fund
  • Fonds du Recherche du Quebec [FRQNT grant number 2014-NC-172924 and FRQS grant number 33273]
  • Centre of Biological Applications of Mass Spectrometry at Concordia University
Digital Object Identifier (DOI):10.3390/toxins11080433
Keywords:mycotoxins; metabolism; high-resolution mass spectrometry (HRMS); microsomal incubation; glucuronidation; human; biomonitoring
ID Code:986098
Deposited By: KRISTA ALEXANDER
Deposited On:13 Nov 2019 22:14
Last Modified:13 Nov 2019 22:14
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