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Neutral and adaptive drivers of genomic change in introduced brook trout (Salvelinus fontinalis) populations revealed by pooled whole-genome re-sequencing

Title:

Neutral and adaptive drivers of genomic change in introduced brook trout (Salvelinus fontinalis) populations revealed by pooled whole-genome re-sequencing

Brookes, Brent (2020) Neutral and adaptive drivers of genomic change in introduced brook trout (Salvelinus fontinalis) populations revealed by pooled whole-genome re-sequencing. Masters thesis, Concordia University.

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Abstract

Understanding the drivers of successful species invasions is important for conserving native biodiversity and for mitigating the economic impacts of introduced species. However, whole genome resolution investigations of the underlying contributions of neutral and adaptive genetic variation to successful colonization in introduced populations are rare. Increased propagule pressure should result in greater neutral genetic variation, while environmental differences should elicit selective pressures on introduced populations, potentially supporting greater adaptive genetic variation. We investigated neutral and adaptive variation among nine introduced brook trout (Salvelinus fontinalis) populations using whole-genome sequencing (28,490,618 SNPs based on pool-seq). The populations inhabit different, isolated lakes in western Canada and descend from a common source, with an average of ~19 (range of 7-41) generations since introduction. We found no evidence of bottleneck events nor strong evidence of purifying selection, and little support that varying propagule pressure or differences in local environments shaped neutral genetic variation. Putative outlier analysis revealed non-convergent patterns of adaptive differentiation among lakes with minimal outlier loci (0.001%-0.15%) which did not correspond with tested environmental variables, despite conditions that should facilitate stronger adaptive differentiation (e.g. abiotic and biotic environmental differences, propagule pressure differences). Our whole genome sequencing analysis provides an example of an introduction success not strongly influenced by genetic variation and suggests that observed differentiation among introduced salmonid populations can be idiosyncratic, population-specific, or stochastic.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Thesis (Masters)
Authors:Brookes, Brent
Institution:Concordia University
Degree Name:M. Sc.
Program:Biology
Date:13 July 2020
Thesis Supervisor(s):Fraser, Dylan
Keywords:pool-seq, pooled sequencing, brook trout, genetics, genomics, introduced species, invasive species, stocking history, genetic variation
ID Code:987160
Deposited By: Brent Brookes
Deposited On:18 Jan 2021 21:15
Last Modified:18 Jan 2021 21:15

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