Ibrahim RK, Anzellotti D: Enzymatic Basis of Flavonoid Diversity. In Integrative Phytochemistry: from ethnobotany to molecular ecology. Volume 37. Edited by: Romeo JT. New York, Plenum Press; 2003:1-36. Ibrahim RK, De Luca V, Khouri H, Latchinian L, Brisson L, Charest PM: Enzymology and compartmentation of polymethylated flavonol glucosides in Chrysosplenium americanum.Phytochemistry 1987 , 26:1237-1249. Anzellotti D, Ibrahim RK: Novel flavonol 2-oxoglutarate dependent dioxygenase: affinity purification, characterization, and kinetic properties.Arch Biochem Biophys 2000 , 382:161-172. Prescott AG, Lloyd MD: The iron(II) and 2-oxoacid-dependent dioxygenases and their role in metabolism.Nat Prod Rep 2000 , 17:367-383. Lukacin R, Urbanke C, Groning I, Matern U: The monomeric polypeptide comprises the functional flavanone 3b-hydroxylase from Petunia hybrida.FEBS Lett 2000 , 467:353-358. Wellmann F, Lukacin R, Moriguchi T, Britsch L, Schiltz E, Matern U: Functional expression and mutational analysis of flavonol synthase from Citrus unshiu. Eur J Biochem 2002 , 269:4134-4142. Saito K, Kobayashi M, Gong Z, Tanaka Y, Yamazaki M: Direct evidence for anthocyanidin synthase as a 2-oxoglutarate-dependent oxygenase: molecular cloning and functional expression of cDNA from a red forma of Perilla frutescens. Plant J 1999 , 17:181-189. Gauthier A, Gulick PJ, Ibrahim RK: cDNA cloning and characterization of a 3'/5'-O-methyltransferase for partially methylated flavonols from Chrysosplenium americanum. Plant Mol Biol 1996 , 32:1163-1169. Lukacin R, Britsch L: Identification of strictly conserved histidine and arginine residues as part of the active site in Petunia hybrida flavanone 3b-hydroxylase. Eur J Biochem 1997 , 249:748-757. Vazquez-Flota F, De Carolis E, Alarco AM, De Luca V: Molecular cloning and characterization of desacetoxyvindoline-4-hydroxylase, a 2-oxoglutarate dependent-dioxygenase involved in the biosynthesis of vindoline in Catharanthus roseus (L.) G. Don. Plant Mol Biol 1997 , 34:935-948. Clare JJ, Romanos MA, Rayment FB, Rowedder JE, Smith MA, Payne MM, Sreekrishna K, Henwood CA: Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies. Gene 1991 , 105:205-212. Lukacin R, Wellmann F, Britsch L, Martens S, Matern U: Flavonol synthase from Citrus unshiu is a bifunctional dioxygenase. Phytochemistry 2003 , 62:287-292. Mehn MP, Fujisawa K, Hegg EL, Que LJ: Oxygen activation by nonheme iron(II) complexes: a-keto carboxylate versus carboxylate. J Am Chem Soc 2003 , 125:7828-7842. Prescott AG, John P: DIOXYGENASES: Molecular Structure and Role in Plant Metabolism. Annu Rev Plant Physiol Plant Mol Biol 1996 , 47:245-271. Liu CJ, Huhman D, Sumner LW, Dixon RA: Regiospecific hydroxylation of isoflavones by cytochrome p450 81E enzymes from Medicago truncatula. Plant J 2003 , 36:471-484. Ibrahim RK: Immunolocalization of Flavonoid Conjugates. In Phenolic Metabolism in Plants. Edited by: Stafford, H. and Ibrahim RK. New York, Plenum Press; 1992:25-61. Martens S, Forkmann G, Britsch L, Wellmann F, Matern U, Lukacin R: Divergent evolution of flavonoid 2-oxoglutarate-dependent dioxygenases in parsley. FEBS Lett 2003 , 544:93-98. Turnbull JJ, Nakajima J, Welford RW, Yamazaki M, Saito K, Schofield CJ: Mechanistic studies on three 2-oxoglutarate-dependent oxygenases of flavonoid biosynthesis: anthocyanidin synthase, flavonol synthase, and flavanone 3beta-hydroxylase. J Biol Chem 2004 , 279:1206-1216. Halbwirth H, Wurst F, Forkmann G, Stich K: Enzymatic A-ring hydroxylation of flavonols at position 8: ; Montreal, Canada. ; 1999. Latunde-Dada AO, Cabello-Hurtado F, Czittrich N, Didierjean L, Schopfer C, Hertkorn N, Werck-Reichhart D, Ebel J: Flavonoid 6-hydroxylase from soybean (Glycine max L.), a novel plant P-450 monooxygenase. J Biol Chem 2001 , 276:1688-1695. Britsch L, Grisebach H: Purification and characterization of (2S)-flavanone 3-hydroxylase from Petunia hybrida. Eur J Biochem 1986 , 156:569-577. Britsch L: Purification and characterization of flavone synthase I, a 2-oxoglutarate-dependent desaturase. Arch Biochem Biophys 1990 , 282:152-160. Holton TA, Brugliera F, Tanaka Y: Cloning and expression of flavonol synthase from Petunia hybrida. Plant J 1993 , 4:1003-1010. Wilmouth RC, Turnbull JJ, Welford RW, Clifton IJ, Prescott AG, Schofield CJ: Structure and mechanism of anthocyanidin synthase from Arabidopsis thaliana. Structure (Camb) 2002 , 10:93-103. Eng CM, Niehaus DJ, Enriquez AL, Burgert TS, Ludman MD, Desnick RJ: Fabry disease: twenty-three mutations including sense and antisense CpG alterations and identification of a deletional hot-spot in the a-galactosidase A gene. Hum Mol Genet 1994 , 3:1795-1799. Chittum HS, Lane WS, Carlson BA, Roller PP, Lung FD, Lee BJ, Hatfield DL: Rabbit b-globin is extended beyond its UGA stop codon by multiple suppressions and translational reading gaps. Biochemistry 1998 , 37:10866-10870. Friedman KD, Rosen NL, Newman PJ, Montgomery RR: Screening of lambda-gt11 libraries. In PCR Protocols: A guide to methods and applications. San Diego, Academic Press; 1990:253-257. Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A 1977 , 74:5463-5467. Seidman JD: Screening of recombinant DNA libraries. In Current Protocols on Molecular Biology. New York, John Wiley and Sons; 1994:6.0.3-6.3.6. Sambrook J, Russell DW: Molecular Cloning: a laboratory manual. 3rd edition. Cold Spring Harbor, Cold Spring Harbor Press; 2001. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970 , 227:680-685. Towbin H, Staehelin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 1979 , 76:4350-4354. Thomson JDHDGGTJ: CLUSTAL W- improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Research 1994 , 22:4673-4680. Maddison DR, Maddison WP: MacClade, Version 4.03. Sunderland, Massachusetts, Sinauer Associates; 2000. Swofford DL: PAUP. Sunderland, Massachusetts, Sinauer Associates; 2000. Saitou N, Nei M: The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987 , 4:406-425.