Barth, G., and T. Scheuber. 1993. Cloning of the isocitrate lyase gene (ICL) from Yarrowia lipolytica and characterization of the deduced protein. Mol. Gen. Genet. 241:422–430. Dalbey, R.E., and C. Robinson. 1999. Protein translocation into and across the bacterial plasma membrane and the plant thylakoid membrane. Trends Biochem. Sci. 24:17–22. Dansen, T.B., K.W.A. Wirtz, R.J.A. Wanders, and E.H.W. Pap. 2000. Peroxisomes in human fibroblasts have a basic pH. Nat. Cell Biol. 2:51–53. Eitzen, G.A., R.K. Szilard, and R.A. Rachubinski. 1997. Enlarged peroxisomes are present in oleic acid-grown Yarrowia lipolytica overexpressing the PEX16 gene encoding an intraperoxisomal peripheral membrane peroxin. J. Cell Biol. 137:1265–1278. Elgersma, Y., A. Vos, M. van den Berg, C.W. van Roermund, P. van der Sluijs, B. Distel, and H.F. Tabak. 1996. Analysis of the carboxyl-terminal peroxisomal targeting signal 1 in a homologous context in Saccharomyces cerevisiae. J. Biol. Chem. 271:26375–26382. Glover, J.R., D.W. Andrews, and R.A. Rachubinski. 1994. Saccharomyces cerevisiae peroxisomal thiolase is imported as a dimer. Proc. Natl. Acad. Sci. USA. 91:10541–10545. Häusler, T., Y. Stierhof, E. Wirtz, and C. Clayton. 1996. Import of DHFR hybrid protein into glycosomes in vivo is not inhibited by the folate-analogue aminopterin. J. Cell Biol. 132:311–324. Kim, J., S.V. Scott, M.N. Oda, and D.J. Klionsky. 1997. Transport of a large oligomeric protein by the cytoplasm to vacuole targeting pathway. J. Cell Biol. 137:609–618. Klionsky, D.J., and Y. Ohsumi. 1999. Vacuolar import of proteins and organelles from the cytoplasm. Annu. Rev. Cell Dev. Biol. 15:1–32. Lee, M.S., R.T. Mullen, and R.N. Trelease. 1997. Oilseed isocitrate lyases lacking their essential type I peroxisomal targeting signal are piggybacked to glyoxysomes. Plant Cell. 9:185–197. Leiper, J.M., P.B. Oatey, and C.J. Danpure. 1996. Inhibition of alanine:glyoxylate aminotransferase 1 dimerization is a prerequisite for its peroxisome-to-mitochondrion mistargeting in primary hyperoxaluria type 1. J. Cell Biol. 135:939–951. Mannaerts, G.P., and P.P. van Veldhoven. 1996. Functions and organization of peroxisomal β-oxidation. Ann. NY Acad. Sci. 804:99–115. McNew, J.A., and J.M. Goodman. 1994. An oligomeric protein is imported into peroxisomes in vivo. J. Cell Biol. 127:1245–1257. McNew, J.A., and J.M. Goodman. 1996. The targeting and assembly of peroxisomal proteins: some old rules do not apply. Trends Biochem. Sci. 21:54–58. Neuvéglise, C., J.M. Nicaud, P. Ross-Macdonald, and C. Gaillardin. 1998. A shuttle mutagenesis system for tagging genes in the yeast Yarrowia lipolytica. Gene. 213:37–46. Rodrigue, A., A. Chanal, K. Beck, M. Müller, and L.-F. Wu. 1999. Co-translocation of a periplasmic enzyme complex by a hitchhiker mechanism through the bacterial Tat pathway. J. Biol. Chem. 274:13223–13228. Santini, C.-L., B. Ize, A. Chanal, M. Müller, G. Giordano, and L.-F. Wu. 1998. A novel Sec-independent periplasmic protein translocation pathway in Escherichia coli. EMBO J. 17:101–112. Schatz, G., and B. Dobberstein. 1996. Common principles of protein translocation across membranes. Science. 271:1519–1526.Abstract↵ Subramani, S. 1998. Components involved in peroxisome import, biogenesis, proliferation, turnover, and movement. Physiol. Rev. 78:171–188. Stewart, M.Q., R.D. Esposito, J. Gowani, and J.M. Goodman. 2001. Alcohol oxidase and dihydroxyacetone synthase, the abundant peroxisomal proteins of methylotrophic yeasts, assemble in different cellular compartments. J. Cell Sci. 114:2863–2868. Szilard, R.K., V.I. Titorenko, M. Veenhuis, and R.A. Rachubinski. 1995. Pay32p of the yeast Yarrowia lipolytica is an intraperoxisomal component of the matrix protein translocation machinery. J. Cell Biol. 131:1453–1469. Teter, S.A., and D.J. Klionsky. 1999. How to get a folded protein across a membrane. Trends Cell Biol. 9:428–431. Teter, S.A., and S.M. Theg. 1998. Energy-transducing thylakoid membranes remain highly impermeable to ions during protein translocation. Proc. Natl. Acad. Sci. USA. 95:1590–1594. Titorenko, V.I., J.J. Smith, R.K. Szilard, and R.A. Rachubinski. 1998. Pex20p of the yeast Yarrowia lipolytica is required for the oligomerization of thiolase in the cytosol and for its targeting to the peroxisome. J. Cell Biol. 142:403–420. Titorenko, V.I., H. Chan, and R.A. Rachubinski. 2000. Fusion of small peroxisomal vesicles in vitro reconstructs an early step in the in vivo multistep peroxisome assembly pathway of Yarrowia lipolytica. J. Cell Biol. 148:29–43. Titorenko, V.I., and R.A. Rachubinski. 2001. The life cycle of the peroxisome. Nat. Rev. Mol. Cell Biol. 2:357–368. van den Bosch, H., R.B.H. Schutgens, R.J.A. Wanders, and J.M. Tager. 1992. Biochemistry of peroxisomes. Annu. Rev. Biochem. 61:157–197. Walton, P.A., P.E. Hill, and S. Subramani. 1995. Import of stably folded proteins into peroxisomes. Mol. Biol. Cell. 6:675–683. Wang, H.J., M.-T. Le Dall, Y. Waché, C. Laroche, J.-M. Belin, C. Gaillardin, and J.-M. Nicaud. 1999a. Evaluation of acyl coenzyme A oxidase (Aox) isozyme function in the n-alkane-assimilating yeast Yarrowia lipolytica. J. Bacteriol. 181:5140–5148. Wang, H.J., M.-T. Le Dall, Y. Waché, C. Laroche, J.-M. Belin, and J.-M. Nicaud. 1999b. Cloning, sequencing, and characterization of five genes coding for acyl-CoA oxidase isozymes of the yeast Yarrowia lipolytica. Cell Biochem. Biophys. 31:165–174. Waterham, H.R., K.A. Russell, Y. de Vries, and J.M. Cregg. 1997. Peroxisomal targeting, import, and assembly of alcohol oxidase in Pichia pastoris. J. Cell Biol. 139:1419–1431.