Feature design is an important part to identify objects of interest into a known number of categories or classes in object detection. Based on the depth-first search for higher order feature functions, the technique of automated feature synthesis is generally considered to be a process of creating more effective features from raw feature data during the run of the algorithms. This dynamic synthesis of nonlinear feature functions is a challenging problem in object detection. This thesis presents a combinatorial approach of genetic programming and the expectation maximization algorithm (GP-EM) to synthesize nonlinear feature functions automatically in order to solve the given tasks of object detection. The EM algorithm investigates the use of Gaussian mixture which is able to model the behaviour of the training samples during an optimal GP search strategy. Based on the Gaussian probability assumption, the GP-EM method is capable of performing simultaneously dynamic feature synthesis and model-based generalization. The EM part of the approach leads to the application of the maximum likelihood (ML) operation that provides protection against inter-cluster data separation and thus exhibits improved convergence. Additionally, with the GP-EM method, an innovative technique, called the histogram region of interest by thresholds (HROIBT), is introduced for diagnosing protein conformation defects (PCD) from microscopic imagery. The experimental results show that the proposed approach improves the detection accuracy and efficiency of pattern object discovery, as compared to single GP-based feature synthesis methods and also a number of other object detection systems. The GP-EM method projects the hyperspace of the raw data onto lower-dimensional spaces efficiently, resulting in faster computational classification processes.