Road mitigation measures serve three general objectives: A. To reduce the risk of traffic mortality of wildlife and to increase traffic safety for drivers; B. To improve the permeability of the highway, i.e. to increase access to habitats on either side of the highway for animals of all species; C. To maintain connectivity of ecological processes across the road and provide for the long‐term persistence of wildlife populations in the area. This research project has three main objectives: (1) To characterize the locations and rates of vehicle collisions with medium‐sized and small mammals and to evaluate the changes in the frequency of highway‐related mortality due to the mitigation measures; (2) To determine the performance of the five types of wildlife passages for medium‐sized and small mammals; (3) To assess how well the mitigation measures provide for the permeability of the highway for individuals and for gene flow across the road, with a focus on American marten.