Automated liquid handlers are devices for automating synthetic biology experiments by moving volumes
in the range of 1μL to 1,000μL depending on the experiment. Most currently available devices utilize
a stepper motor-based 3-axis system and linear actuated pump. These devices can be set up to automate
hundreds or thousands of manual liquid handling steps. These liquid handling steps are both substantially
repetitive and often introduce human error. As such, automation can be used to save time and eliminate human
error. In this thesis, we describe a low-cost, open-source, automated liquid handler, the BioCloneBot,
designed to automate pick and place DNA assembly experiments. The BioCloneBot consists of off-the-shelf
and 3D-printed components. To reduce complexity and reduce sourcing multiple parts, an Ender 3 Pro 3D
printer is used for its frame, stepper motors, and limit switches. The result is a highly accurate and precise
liquid handler with an intuitive front-end making automation more accessible by lowering the cost to less
than $1, 500 CAD.