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Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88


Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88

Andersen, M. R., Salazar, M. P., Schaap, P. J., van de Vondervoort, P. J. I., Culley, D., Thykaer, J., Frisvad, J. C., Nielsen, K. F., Albang, R., Albermann, K., Berka, R. M., Braus, G. H., Braus-Stromeyer, S. A., Corrochano, L. M., Dai, Z., van Dijck, P. W. M., Hofmann, G., Lasure, L. L., Magnuson, J. K., Menke, H., Meijer, M., Meijer, S. L., Nielsen, J. B., Nielsen, M. L., van Ooyen, A. J. J., Pel, H. J., Poulsen, L., Samson, R. A., Stam, H., Tsang, Adrian, van den Brink, J. M., Atkins, A., Aerts, A., Shapiro, H., Pangilinan, J., Salamov, A., Lou, Y., Lindquist, E., Lucas, S., Grimwood, J., Grigoriev, I. V., Kubicek, C. P., Martinez, D., van Peij, N. N. M. E., Roubos, J. A., Nielsen, J. and Baker, S. E. (2011) Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88. Genome Research, 21 (6). pp. 885-897. ISSN 1088-9051

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Official URL: http://dx.doi.org/10.1101/gr.112169.110


The filamentous fungus Aspergillus niger exhibits great diversity in its phenotype. It is found globally, both as marine and terrestrial strains, produces both organic acids and hydrolytic enzymes in high amounts, and some isolates exhibit pathogenicity. Although the genome of an industrial enzyme-producing A. niger strain (CBS 513.88) has already been sequenced, the versatility and diversity of this species compel additional exploration. We therefore undertook whole-genome sequencing of the acidogenic A. niger wild-type strain (ATCC 1015) and produced a genome sequence of very high quality. Only 15 gaps are present in the sequence, and half the telomeric regions have been elucidated. Moreover, sequence information from ATCC 1015 was used to improve the genome sequence of CBS 513.88. Chromosome-level comparisons uncovered several genome rearrangements, deletions, a clear case of strain-specific horizontal gene transfer, and identification of 0.8 Mb of novel sequence. Single nucleotide polymorphisms per kilobase (SNPs/kb) between the two strains were found to be exceptionally high (average: 7.8, maximum: 160 SNPs/kb). High variation within the species was confirmed with exo-metabolite profiling and phylogenetics. Detailed lists of alleles were generated, and genotypic differences were observed to accumulate in metabolic pathways essential to acid production and protein synthesis. A transcriptome analysis supported up-regulation of genes associated with biosynthesis of amino acids that are abundant in glucoamylase A, tRNA-synthases, and protein transporters in the protein producing CBS 513.88 strain. Our results and data sets from this integrative systems biology analysis resulted in a snapshot of fungal evolution and will support further optimization of cell factories based on filamentous fungi.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Article
Authors:Andersen, M. R. and Salazar, M. P. and Schaap, P. J. and van de Vondervoort, P. J. I. and Culley, D. and Thykaer, J. and Frisvad, J. C. and Nielsen, K. F. and Albang, R. and Albermann, K. and Berka, R. M. and Braus, G. H. and Braus-Stromeyer, S. A. and Corrochano, L. M. and Dai, Z. and van Dijck, P. W. M. and Hofmann, G. and Lasure, L. L. and Magnuson, J. K. and Menke, H. and Meijer, M. and Meijer, S. L. and Nielsen, J. B. and Nielsen, M. L. and van Ooyen, A. J. J. and Pel, H. J. and Poulsen, L. and Samson, R. A. and Stam, H. and Tsang, Adrian and van den Brink, J. M. and Atkins, A. and Aerts, A. and Shapiro, H. and Pangilinan, J. and Salamov, A. and Lou, Y. and Lindquist, E. and Lucas, S. and Grimwood, J. and Grigoriev, I. V. and Kubicek, C. P. and Martinez, D. and van Peij, N. N. M. E. and Roubos, J. A. and Nielsen, J. and Baker, S. E.
Journal or Publication:Genome Research
Date:4 May 2011
Digital Object Identifier (DOI):10.1101/gr.112169.110
ID Code:7715
Deposited On:11 Jul 2011 14:33
Last Modified:18 Jan 2018 17:31


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