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The Interaction of Streptococcal Enolase with Canine Plasminogen: The Role of Surfaces in Complex Formation

Title:

The Interaction of Streptococcal Enolase with Canine Plasminogen: The Role of Surfaces in Complex Formation

Balhara, Vinod, Deshmukh, Sasmit S., Kálmán, László and Kornblatt, Jack A. (2014) The Interaction of Streptococcal Enolase with Canine Plasminogen: The Role of Surfaces in Complex Formation. PLoS ONE, 9 (2). e88395. ISSN 1932-6203

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Official URL: http://dx.doi.org/10.1371/journal.pone.0088395

Abstract

The enolase from Streptococcus pyogenes (Str enolase F137L/E363G) is a homo-octamer shaped like a donut. Plasminogen (Pgn) is a monomeric protein composed of seven discrete separated domains organized into a lock washer. The enolase is known to bind Pgn. In past work we searched for conditions in which the two proteins would bind to one another. The two native proteins in solution would not bind under any of the tried conditions. We found that if the structures were perturbed binding would occur. We stated that only the non-native Str enolase or Pgn would interact such that we could detect binding. We report here the results of a series of dual polarization interferometry (DPI) experiments coupled with atomic force microscopy (AFM), isothermal titration calorimetry (ITC), dynamic light scattering (DLS), and fluorescence. We show that the critical condition for forming stable complexes of the two native proteins involves Str enolase binding to a surface. Surfaces that attract Str enolase are a sufficient condition for binding Pgn. Under certain conditions, Pgn adsorbed to a surface will bind Str enolase.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Article
Refereed:Yes
Authors:Balhara, Vinod and Deshmukh, Sasmit S. and Kálmán, László and Kornblatt, Jack A.
Journal or Publication:PLoS ONE
Date:2014
Funders:
  • Concordia Open Access Author Fund
Digital Object Identifier (DOI):10.1371/journal.pone.0088395
ID Code:978295
Deposited By: DAVID MACAULAY
Deposited On:27 Feb 2014 20:49
Last Modified:18 Jan 2018 17:46
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