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Glucocorticoids and Stress-Induced Changes in the Expression of PERIOD1 in the Rat Forebrain

Title:

Glucocorticoids and Stress-Induced Changes in the Expression of PERIOD1 in the Rat Forebrain

Al-Safadi, Sherin, Branchaud, Marie, Rutherford, Spencer and Amir, Shimon (2015) Glucocorticoids and Stress-Induced Changes in the Expression of PERIOD1 in the Rat Forebrain. PLOS ONE, 10 (6). e0130085.

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Official URL: http://dx.doi.org/10.1371/journal.pone.0130085

Abstract

The secretion of glucocorticoids in mammals is under circadian control, but glucocorticoids themselves are also implicated in modulating circadian clock gene expression. We have shown that the expression of the circadian clock protein PER1 in the forebrain is modulated by stress, and that this effect is associated with changes in plasma corticosterone levels, suggesting a possible role for glucocorticoids in the mediation of stress-induced changes in the expression of PER1 in the brain. To study this, we assessed the effects of adrenalectomy and of pretreatment with the glucocorticoid receptor antagonist, mifepristone, on the expression of PER1 in select limbic and hypothalamic regions following acute exposure to a neurogenic stressor, restraint, or a systemic stressor, 2-Deoxy-D-glucose (2DG) in rats. Acute restraint suppressed PER1 expression in the oval nucleus of the bed nucleus of the stria terminalis (BNSTov) and the central nucleus of the amygdala (CEAl), whereas 2DG increased PER1 in both regions. Both stressors increased PER1 expression in the paraventricular (PVN) and dorsomedial (DMH) nuclei of the hypothalamus, and the piriform cortex (Pi). Adrenalectomy and pretreatment with mifepristone reversed the effects of both stressors on PER1 expression in the BNSTov and CEAl, and blocked their effects in the DMH. In contrast, both treatments enhanced the effects of restraint and 2DG on PER1 levels in the PVN. Stress-induced PER1 expression in the Pi was unaffected by either treatment. PER1 expression in the suprachiasmatic nucleus, the master circadian clock, was not altered by either exposure to stress or by the glucocorticoid manipulations. Together, the results demonstrate a key role for glucocorticoid signaling in stress-induced changes in PER1 expression in the brain.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Article
Refereed:Yes
Authors:Al-Safadi, Sherin and Branchaud, Marie and Rutherford, Spencer and Amir, Shimon
Journal or Publication:PLOS ONE
Date:15 June 2015
Funders:
  • Concordia Open Access Author Fund
Digital Object Identifier (DOI):10.1371/journal.pone.0130085
ID Code:980122
Deposited By: SHIMON AMIR
Deposited On:25 Jun 2015 15:10
Last Modified:18 Jan 2018 17:50
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