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Characterization of Tbc2, a nucleus-encoded factor specifically required for translation of the chloroplast psbC mRNA in Chlamydomonas reinhardtii


Characterization of Tbc2, a nucleus-encoded factor specifically required for translation of the chloroplast psbC mRNA in Chlamydomonas reinhardtii

Auchincloss, A. H., Zerges, William, Perron, Karl, Girard-Bascou, Jacqueline and Rochaix, Jean-David (2002) Characterization of Tbc2, a nucleus-encoded factor specifically required for translation of the chloroplast psbC mRNA in Chlamydomonas reinhardtii. The Journal of Cell Biology, 157 (6). pp. 953-962. ISSN 00219525

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Official URL: http://dx.doi.org/10.1083/jcb.200201060


Genetic analysis has revealed that the three nucleus-encoded factors Tbc1, Tbc2, and Tbc3 are involved in the translation of the chloroplast psbC mRNA of the eukaryotic green alga Chlamydomonas reinhardtii. In this study we report the isolation and phenotypic characterization of two new tbc2 mutant alleles and their use for cloning and characterizing the Tbc2 gene by genomic complementation. TBC2 encodes a protein of 1,115 residues containing nine copies of a novel degenerate 38–40 amino acid repeat with a quasiconserved PPPEW motif near its COOH-terminal end. The middle part of the Tbc2 protein displays partial amino acid sequence identity with Crp1, a protein from Zea mays that is implicated in the processing and translation of the chloroplast petA and petD RNAs. The Tbc2 protein is enriched in chloroplast stromal subfractions and is associated with a 400-kD protein complex that appears to play a role in the translation of specifically the psbC mRNA.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Article
Authors:Auchincloss, A. H. and Zerges, William and Perron, Karl and Girard-Bascou, Jacqueline and Rochaix, Jean-David
Journal or Publication:The Journal of Cell Biology
Date:10 June 2002
Digital Object Identifier (DOI):10.1083/jcb.200201060
ID Code:7606
Deposited By: Danielle Dennie
Deposited On:16 May 2011 15:42
Last Modified:18 Jan 2018 17:31


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