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Low Density Membranes Are Associated with RNA-binding Proteins and Thylakoids in the Chloroplast of Chlamydomonas reinhardtii


Low Density Membranes Are Associated with RNA-binding Proteins and Thylakoids in the Chloroplast of Chlamydomonas reinhardtii

Zerges, William and Rochaix, Jean-David (1998) Low Density Membranes Are Associated with RNA-binding Proteins and Thylakoids in the Chloroplast of Chlamydomonas reinhardtii. The Journal of Cell Biology, 140 (1). pp. 101-110. ISSN 00219525 (In Press)

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Official URL: http://dx.doi.org/10.1083/jcb.140.1.101


Chloroplast subfractions were tested with a UV cross-linking assay for proteins that bind to the 5′ untranslated region of the chloroplast psbC mRNA of the green alga Chlamydomonas reinhardtii. These analyses revealed that RNA-binding proteins of 30–32, 46, 47, 60, and 80 kD are associated with chloroplast membranes. The buoyant density and the acyl lipid composition of these membranes are compatible with their origin being the inner chloroplast envelope membrane. However, unlike previously characterized inner envelope membranes, these membranes are associated with thylakoids. One of the membrane-associated RNA-binding proteins appears to be RB47, which has been reported to be a specific activator of psbA mRNA translation. These results suggest that translation of chloroplast mRNAs encoding thylakoid proteins occurs at either a subfraction of the chloroplast inner envelope membrane or a previously uncharacterized intra-chloroplast compartment, which is physically associated with thylakoids.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Article
Authors:Zerges, William and Rochaix, Jean-David
Journal or Publication:The Journal of Cell Biology
Date:12 January 1998
Digital Object Identifier (DOI):10.1083/jcb.140.1.101
ID Code:7607
Deposited By: Danielle Dennie
Deposited On:16 May 2011 15:51
Last Modified:18 Jan 2018 17:31


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