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Interference of fetal hemoglobin in the determination of carboxyhemoglobin by spectrophotometry


Interference of fetal hemoglobin in the determination of carboxyhemoglobin by spectrophotometry

Vaillancourt, Lucie, Desharnais, Brigitte ORCID: https://orcid.org/0000-0001-7373-656X, Goudreau, Nathalie and Mireault, Pascal (2015) Interference of fetal hemoglobin in the determination of carboxyhemoglobin by spectrophotometry. Canadian Society of Forensic Science Journal, 49 (2). pp. 69-77. ISSN 0008-5030

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Official URL: https://doi.org/10.1080/00085030.2015.1115692


Determination of carboxyhemoglobin (HbCO) is routinely performed in suspected cases of carbon monoxide intoxication and unexplained deaths. However, some authors have suggested that measured HbCO may be falsely elevated in infants (0–12 months) due to the presence of fetal hemoglobin (HbF). The purpose of this study was to evaluate the impact of fetal hemoglobin on the spectrophotometric determination of carboxyhemoglobin. The interference of HbF in the determination of HbCO in infants aged from 0 to 12 months was evaluated using 16 ante-mortem and 19 post-mortem blood samples. The %HbCO was quantified spectrophotometrically by calculating the 560 nm/530 nm absorbance ratio, using a dual beam spectrophotometer. The average measured HbCO in infants of 3 months of age or under was 17%, which is abnormally elevated. No significant difference in HbCO measurement was found between ante-mortem and post-mortem samples. These results highlight the fact that care must be taken in interpretation of carboxyhemoglobin measurements in infants when using a spectrophotometric method.

Divisions:Concordia University > Faculty of Arts and Science > Chemistry and Biochemistry
Item Type:Article
Authors:Vaillancourt, Lucie and Desharnais, Brigitte and Goudreau, Nathalie and Mireault, Pascal
Journal or Publication:Canadian Society of Forensic Science Journal
Date:30 November 2015
Digital Object Identifier (DOI):10.1080/00085030.2015.1115692
Keywords:Forensic toxicology, carboxyhemoglobin, fetal hemoglobin, spectrophotometry, neonates, false positive
ID Code:986823
Deposited On:21 May 2020 15:28
Last Modified:27 May 2020 21:59
Related URLs:
Additional Information:La version française https://spectrum.library.concordia.ca/986824/


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