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Microbial life under ice: Metagenome diversity and in situ activity of Verrucomicrobia in seasonally ice‐covered lakes

Title:

Microbial life under ice: Metagenome diversity and in situ activity of Verrucomicrobia in seasonally ice‐covered lakes

Tran, Patricia ORCID: https://orcid.org/0000-0003-3948-3938 (2018) Microbial life under ice: Metagenome diversity and in situ activity of Verrucomicrobia in seasonally ice‐covered lakes. Masters thesis, Concordia University.

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Abstract

Northern lakes are ice‐covered for a large part of the year, yet our understanding of microbial diversity and activity during winter lags behind that of the ice‐free period. In this study, we investigated under‐ice diversity and metabolism of Verrucomicrobia in seasonally ice‐covered lakes in temperate and boreal regions of Quebec, Canada using 16S rRNA sequencing, metagenomics and metatranscriptomics. Verrucomicrobia, particularly the V1, V3 and V4 subdivisions, were abundant during ice‐covered periods. A diversity of Verrucomicrobia genomes were reconstructed from Quebec lake metagenomes. Several genomes were associated with the ice‐covered period and were represented in winter metatranscriptomes, supporting the notion that Verrucomicrobia are metabolically active under ice. Verrucomicrobia transcriptome analysis revealed a range of metabolisms potentially occurring under ice, including carbohydrate degradation, glycolate utilization, scavenging of chlorophyll degradation products, and urea use. Genes for aerobic sulfur and hydrogen oxidation were expressed, suggesting chemolithotrophy may be an adaptation to conditions where labile carbon may be limited. The expression of genes for flagella biosynthesis and chemotaxis was detected, suggesting Verrucomicrobia may be actively sensing and responding to winter nutrient pulses, such as phytoplankton blooms. These results increase our understanding on the diversity and metabolic processes occurring under ice in northern lakes ecosystems.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Thesis (Masters)
Authors:Tran, Patricia
Institution:Concordia University
Degree Name:M. Sc.
Program:Biology
Date:June 2018
Thesis Supervisor(s):Walsh, David
Keywords:limnology, microbial ecology, freshwater science, metagenomics
ID Code:984564
Deposited By: Patricia Tran
Deposited On:16 Nov 2018 17:03
Last Modified:16 Nov 2018 17:03
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  • Microbial life under ice: Metagenome diversity and in situ activity of Verrucomicrobia in seasonally ice‐covered lakes. (deposited 16 Nov 2018 17:03) [Currently Displayed]
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