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A threshold LC–MS/MS method for 92 analytes in oral fluid collected with the Quantisal® device

Title:

A threshold LC–MS/MS method for 92 analytes in oral fluid collected with the Quantisal® device

Desharnais, Brigitte ORCID: https://orcid.org/0000-0001-7373-656X, Lajoie, Marie-Jo, Laquerre, Julie, Mireault, Pascal and Skinner, Cameron D. (2019) A threshold LC–MS/MS method for 92 analytes in oral fluid collected with the Quantisal® device. Forensic Science International, 317 . p. 110506. ISSN 0379-0738

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Official URL: https://doi.org/10.1016/j.forsciint.2020.110506

Abstract

A study of impaired driving rates in the province of Québec is currently planned following the legalization of recreational cannabis in Canada. Oral fluid (OF) samples are to be collected with a Quantisal® device and sent to the laboratory for analysis. In order to prepare for this project, a qualitative decision point analysis method monitoring for the presence of 97 drugs and metabolites in OF was developed and validated.

This high throughput method uses incubation with a precipitation solvent (acetone:acetonitrile 30:70 v:v) to boost drug recovery from the collecting device and improve stability of benzodiazepines (e.g., α-hydroxyalprazolam, clonazepam, 7-aminoclonazepam, flunitrazepam, 7-aminoflunitrazepam, N-desmethylflunitrazepam, nitrazepam). The Quantisal® device has polyglycol in its stabilizing buffer, but timed use of the mass spectrometer waste valve proved sufficient to avoid the glycol interferences for nearly all analytes. Interferences from OF matrices and 140 potentially interfering compounds, carryover, ion ratios, stability, recovery, reproducibility, robustness, false positive rate, false negative rate, selectivity, sensitivity and reliability rates were tested in the validation process. Five of the targeted analytes (olanzapine, oxazepam, 7-aminoclonazepam, flunitrazepam and nitrazepam) did not meet the set validation criteria but will be monitored for identification purposes (no comparison to a cut-off level).

Blind internal proficiency testing was performed, where six OF samples were tested and analytes were classified as “negative”, “likely positive” or “positive” with success. The final validated OF qualitative decision point method covers 92 analytes, and the presence of 5 additional analytes is screened in this high throughput analysis.

Divisions:Concordia University > Faculty of Arts and Science > Chemistry and Biochemistry
Item Type:Article
Refereed:Yes
Authors:Desharnais, Brigitte and Lajoie, Marie-Jo and Laquerre, Julie and Mireault, Pascal and Skinner, Cameron D.
Journal or Publication:Forensic Science International
Date:13 September 2019
Projects:
  • Qualitative method validation guidelines
Funders:
  • National Sciences and Engineering Research Council of Canada
  • Fonds de recherche du Québec - Nature et technologies
Digital Object Identifier (DOI):10.1016/j.forsciint.2020.110506
Keywords:Qualitative decision point methods, oral fluid, saliva, Quantisal, liquid chromatography mass spectrometry, roadside survey
ID Code:987845
Deposited By: BRIGITTE DESHARNAIS
Deposited On:15 Jan 2021 21:37
Last Modified:08 Sep 2021 01:00
Related URLs:

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