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A study of the metK gene encoding S-adenosylmethionine synthetase in Escherichia coli K-12

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A study of the metK gene encoding S-adenosylmethionine synthetase in Escherichia coli K-12

Wei, Yuhong (2001) A study of the metK gene encoding S-adenosylmethionine synthetase in Escherichia coli K-12. Masters thesis, Concordia University.

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Abstract

The metK gene of Escherichia coli encodes S-adenosylmethionine synthetase, which catalyzes the reaction between L-methionine and ATP to form S-adenosylmethionine (SAM), the universal methyl group donor in the cell. SAM synthetase is generally assumed to be essential in E. coli , but this has not been proven. I demonstrated here that the metK gene could be deleted in E. coli by gene replacement only in the presence of a rescue plasmid carrying a functional copy of metK . One commonly used metK mutant " metK84 " had a markedly reduced SAM synthetase level and exhibited a complex phenotype under certain growth conditions. Through DNA sequencing analysis and primer extension analysis, I showed that this mutant carried an A[arrow right]G transition in the -10 region of the metK promoter. In this work, the initiation site for metK transcription was determined by primer extension. (Abstract shortened by UMI.)

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Thesis (Masters)
Authors:Wei, Yuhong
Pagination:xiv, 108 leaves : ill. ; 29 cm.
Institution:Concordia University
Degree Name:Theses (M.Sc.)
Program:Biology
Date:2001
Thesis Supervisor(s):Newman, E. B
ID Code:1492
Deposited By:Concordia University Libraries
Deposited On:27 Aug 2009 13:19
Last Modified:08 Dec 2010 10:21
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