Breadcrumb

 
 

Characterization and Development of Novel Stationary Phases for Capillary Electrochromatography

Title:

Characterization and Development of Novel Stationary Phases for Capillary Electrochromatography

Kaltcheva, Maria (2011) Characterization and Development of Novel Stationary Phases for Capillary Electrochromatography. Masters thesis, Concordia University.

[img]
Preview
PDF - Accepted Version
8Mb

Abstract

Porous monolithic stationary phases for capillary electrochromatography have shown great potential due to the ability to tune their properties for tailor-made separations by careful selection of the polymerization parameters. However, the final morphological and chemical properties of the column remain difficult to predict. In order to better understand the effects of the relevant variables on column properties and morphology we have significantly streamlined the preparation procedure and investigated an array of variables (temperature, reaction time, porogenic solvent concentration) on the porosity and retention of the columns. Factorial experimental design was implemented to derive models that were able to describe the polymer porosity and the retention of three PAH’s as a function of starting reagents and conditions.
The current study showed that with a factorial design approach, monolith porosity and chromatography can be reliably tuned by adjusting the porogenic solvent concentration, the polymerization time and reaction temperature. Scanning electron microscopy revealed that the polymerization conditions affected the polymer structure and particle size. Synergistic effects not accessible by the “one variable at time” approach were identified between the temperature and polymerization time and temperature and monomer/porogenic solvent ratio through multivariate analysis. The control of the monolith porosity and chromatographic behaviour will allow future efforts to be focused on creating reproducible, tailor-made monolithic columns with targeted chromatographic properties suitable for the separation of peptides and proteins from biological fluids.

Divisions:Concordia University > Faculty of Arts and Science > Chemistry and Biochemistry
Item Type:Thesis (Masters)
Authors:Kaltcheva, Maria
Institution:Concordia University
Degree Name:M. Sc.
Program:Chemistry
Date:01 April 2011
Thesis Supervisor(s):Skinner, Cameron
Keywords:capillary electrochromatography, CEC, monoliths, factorial design
ID Code:7242
Deposited By:MARIA KALTCHEVA
Deposited On:13 Jun 2011 09:12
Last Modified:13 Jun 2011 09:12
References:
[1] Strain, H. H., Journal of the American Chemical Society 1939, 61, 1292-1293.
[2] Strain, H. H., Sullivan, J. C., Analytical Chemistry 1951, 23, 816-823.
[3] Pretorius, V., Hopkins, B. J., Schieke, J. D., Journal of Chromatography A 1974, 99, 23-30.
[4] Jorgenson, J. W., Lukacs, K. D., Journal of Chromatography A 1981, 218, 209-216.
[5] Svec, F., Majors, R. E., LC-GC North America 2009, 27, 1032-1039.
[6] Svec, F., ELECTROPHORESIS 2009, 30, S68-S82.
[7] Svec, F., Peters, E. C., Sýkora, D., Fréchet, J. M. J., Journal of Chromatography A 2000, 887, 3-29.
[8] in: Atlas, S. (Ed.), Magnetic resonance imaging of the brain and spine, Lippincott Williams & Wilkins, Philadelphia 2009.
[9] Remcho, V. T., The Chemical Educator 1997, 2, 1-16.
[10] Journal of the American Chemical Society 2001, 123, 10427-10427.
[11] Capillary Electrochromatography, Royal Society of Chemistry, Cambridge 1992.
[12] Nguyen, A., Moini, M., Analytical Chemistry 2008, 80, 7169-7173.
[13] Knox, J., Chromatographia 1988, 26, 329-337.
[14] Bartle, K. D., Carney, R. A., Cavazza, A., Cikalo, M. G., Myers, P., Robson, M. M., Roulin, S. C. P., Sealey, K., Journal of Chromatography A 2000, 892, 279-290.
[15] Deyl, Z., Svec, F. eds., Capillary Electrochromatography, Elsevier, Amsterdam 2001.
[16] Bartle, K. D., Myers, P., Journal of Chromatography A 2001, 916, 3-23.
[17] Choudhary, G., Horváth, C., Journal of Chromatography A 1997, 781, 161-183.
[18] Wan, Q.-H., Analytical Chemistry 1997, 69, 361-363.
[19] Snyder, L. R., Kirkland, J.J., Glajch, J.L., Practical HPLC method development, John Wiley & Sons 1997.
[20] Knox, J. H., Journal of Chromatography A 1999, 831, 3-15.
[21] Rathore, A. S., Horváth, C., Analytical Chemistry 1998, 70, 3271-3274.
[22] Pesek, J. J., Matyska, M. T., Dawson, G. B., Chen, Boysen, R. I., Hearn, M. T. W., Analytical Chemistry 2003, 76, 23-30.
[23] Dong, X., Wu, R. a., Dong, J., Wu, M., Zhu, Y., Zou, H., ELECTROPHORESIS 2009, 30, 141-154.
[24] Hilder, E. F., Svec, F., Fréchet, J. M. J., ELECTROPHORESIS 2002, 23, 3934-3953.
[25] Eeltink, S., Svec, F., ELECTROPHORESIS 2007, 28, 137-147.
[26] Ngola, S. M., Fintschenko, Y., Choi, W.-Y., Shepodd, T. J., Analytical Chemistry 2001, 73, 849-856.
[27] Bandilla, D., Skinner, C. D., Journal of Chromatography A 2004, 1044, 113-129.
[28] Lin, W., Department of Chemistry and Biochemistry, Concordia University, Montreal 2009.
[29] Lewis, F. D., Lauterbach, R. T., Heine, H. G., Hartmann, W., Rudolph, H., Journal of the American Chemical Society 1975, 97, 1519-1525.
[30] Progent, F., Augustin, V., Tran, N. T., Descroix, S., Taverna, M., ELECTROPHORESIS 2006, 27, 757-767.
[31] Archie, G. E., Trans. AIME 1942, 146, 54.
[32] Gusev, I., Huang, X., Horváth, C., Journal of Chromatography A 1999, 855, 273-290.
[33] Rathore, A. S., Wen, E., Horváth, C., Analytical Chemistry 1999, 71, 2633-2641.
[34] Svec, F., Frechet, J. M. J., Macromolecules 1995, 28, 7580-7582.
[35] Svec, F., Frechet, J. M. J., Chemistry of Materials 1995, 7, 707-715.
[36] Lu, M., Feng, Q., Lu, Q., Cai, Z., Zhang, L., Chen, G., ELECTROPHORESIS 2009, 30, 3540-3547.
[37] Eeltink, S., Herrero-Martinez, J. M., Rozing, G. P., Schoenmakers, P. J., Kok, W. T., Analytical Chemistry 2005, 77, 7342-7347.
[38] Viklund, C., Svec, F., Frechet, J. M. J., Irgum, K., Chemistry of Materials 1996, 8, 744-750.
[39] Viklund, C., Ponten, E., Glad, B., Irgum, K., Horstedt, P., Svec, F., Chemistry of Materials 1997, 9, 463-471.
[40] Jiang, T., Jiskra, J., Claessens, H. A., Cramers, C. A., Journal of Chromatography A 2001, 923, 215-227.
[41] Leardi, R., Anal Chim Acta 2009, 652, 161-172.
[42] Araujo, P. W., Brereton, R. G., TrAC Trends in Analytical Chemistry 1996, 15, 26-31.
[43] Araujo, P. W., Brereton, R. G., TrAC Trends in Analytical Chemistry 1996, 15, 63-70.
[44] Araujo, P. W., Brereton, R. G., TrAC Trends in Analytical Chemistry 1996, 15, 156-163.
[45] Kramer, R., Chemometric Techniques for Quantitative Analysis, Marcel Dekker, New York 1998.
[46] Cabral, J.-L., Bandilla, D., Skinner, C. D., Journal of Chromatography A 2006, 1108, 83-89.
[47] Al-Bokari, M., Cherrak, D., Guiochon, G., Journal of Chromatography A 2002, 975, 275-284.
[48] Jancǒ, M., Xie, S., Peterson, D. S., Allington, R. W., Svec, F., Fréchet, J. M. J., Journal of Separation Science 2002, 25, 909-916.
[49] Rohr, T., Hilder, E. F., Donovan, J. J., Svec, F., Fréchet, J. M. J., Macromolecules 2003, 36, 1677-1684.
[50] Jalili, N., Laxminarayana, K., Mechatronics 2004, 14, 907-945.
[51] Kornysova, O., Surna, R., Snitka, V., Pyell, U., Maruska, A., Journal of Chromatography A 2002, 971, 225-235.
[52] De La Rue, R. E., Tobias, C. W., Journal of The Electrochemical Society 1959, 106, 827-833.
[53] Huang, X., Horváth, C., Journal of Chromatography A 1997, 788, 155-164.
[54] Cabral, J.-L., Department of Chemistry, Concordia University, Montreal 2008, p. 275.
[55] Decker, C., Decker, D., Morel, F., Photopolymerization, American Chemical Society 1997, pp. 63-80.
[56] Ueki, Y., Umemura, T., Iwashita, Y., Odake, T., Haraguchi, H., Tsunoda, K.-i., Journal of Chromatography A 2006, 1106, 106-111.
[57] Davies, M. P., De Biasi, V., Perrett, D., Analytica Chimica Acta 2004, 504, 7-14.
[58] Iqbal, A., Khan, A.,, Am. J. Eng. Appl. Sci. 2010, 3, 611-619.
[59] Niedz, R. P., Hyndman, S. E., Evens, T. J., Scientia Horticulturae 2007, 112, 349-359.
[60] Gomase, V. S., Kale, K. V., Tagore, S., Hatture, S. R., Current drug metabolism 2008, 9(3), 213-220.
[61] Sleno, L., Emili, A., Current Opinion in Chemical Biology 2008, 12, 46-54.
[62] Ngola, S. M., Fintschenko, Y., Choi, W. Y., Shepodd, T. J., Analytical Chemistry 2001, 73, 849-856.
[63] Bandilla, D., Skinner, C. D., Journal of Chromatography A 2003, 1004, 167-179.
[64] Bandilla, D., Department of Chemisty and Biochemistry, Concordia University, Montreal 2004.
[65] Rassi, Z. E., Electrophoresis 2010, 31, 174-191.


All items in Spectrum are protected by copyright, with all rights reserved. The use of items is governed by Spectrum's terms of access.

Repository Staff Only: item control page

Document Downloads

More statistics for this item...

Concordia University - Footer