Boukh-Viner, Tatiana and Guo, Tong and Alexandrian, Alex and Cerracchio, André and Gregg, Christopher and Haile, Sandra and Kyskan, Robert and Milijevic, Svetlana and Oren, Daniel and Solomon, Jonathan and Wong, Vivianne and Nicaud, Jean-Marc and Rachubinski, Richard A. and English, Ann M. and Titorenko, Vladimir I. (2005) Dynamic ergosterol- and ceramide-rich domains in the peroxisomal membrane serve as an organizing platform for peroxisome fusion. The Journal of Cell Biology, 168 (5). pp. 761-773. ISSN 0021-9525
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Official URL: http://dx.doi.org/10.1083/jcb.200409045
We describe unusual ergosterol- and ceramide-rich (ECR) domains in the membrane of yeast peroxisomes. Several key features of these detergent-resistant domains, including the nature of their sphingolipid constituent and its unusual distribution across the membrane bilayer, clearly distinguish them from well characterized detergent-insoluble lipid rafts in the plasma membrane. A distinct set of peroxisomal proteins, including two ATPases, Pex1p and Pex6p, as well as phosphoinositide- and GTP-binding proteins, transiently associates with the cytosolic face of ECR domains. All of these proteins are essential for the fusion of the immature peroxisomal vesicles P1 and P2, the earliest intermediates in a multistep pathway leading to the formation of mature, metabolically active peroxisomes. Peroxisome fusion depends on the lateral movement of Pex1p, Pex6p, and phosphatidylinositol-4,5-bisphosphate–binding proteins from ECR domains to a detergent-soluble portion of the membrane, followed by their release to the cytosol. Our data suggest a model for the multistep reorganization of the multicomponent peroxisome fusion machinery that transiently associates with ECR domains.
|Divisions:||Concordia University > Faculty of Arts and Science > Biology|
|Authors:||Boukh-Viner, Tatiana and Guo, Tong and Alexandrian, Alex and Cerracchio, André and Gregg, Christopher and Haile, Sandra and Kyskan, Robert and Milijevic, Svetlana and Oren, Daniel and Solomon, Jonathan and Wong, Vivianne and Nicaud, Jean-Marc and Rachubinski, Richard A. and English, Ann M. and Titorenko, Vladimir I.|
|Journal or Publication:||The Journal of Cell Biology|
|Date:||28 February 2005|
|Deposited By:||DANIELLE DENNIE|
|Deposited On:||11 May 2011 12:37|
|Last Modified:||11 May 2011 12:37|
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