Transcription Profiling of Candida albicans Cells Undergoing the Yeast-to-Hyphal Transition


Transcription Profiling of Candida albicans Cells Undergoing the Yeast-to-Hyphal Transition

Nantel, André and Dignard, Daniel and Bachewich, Catherine and Harcus, Doreen and Marcil, Anne and Bouin, Anne-Pascale and Sensen, Christoph W. and Hogues, Hervé and van het Hoog, Marco and Gordon, Paul and Rigby, Tracey and Benoit, Francois and Tessier, Daniel C. and Thomas, David Y. and Whiteway, Malcolm (2002) Transcription Profiling of Candida albicans Cells Undergoing the Yeast-to-Hyphal Transition. Molecular Biology of the Cell, 13 (10). pp. 3452-3465. ISSN 10591524

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The ability of the pathogenic fungus Candida albicans to switch from a yeast to a hyphal morphology in response to external signals is implicated in its pathogenicity. We used glass DNA microarrays to investigate the transcription profiles of 6333 predicted ORFs in cells undergoing this transition and their responses to changes in temperature and culture medium. We have identified several genes whose transcriptional profiles are similar to those of known virulence factors that are modulated by the switch to hyphal growth caused by addition of serum and a 37°C growth temperature. Time course analysis of this transition identified transcripts that are induced before germ tube initiation and shut off later in the developmental process. A strain deleted for the Efg1p and Cph1p transcription factors is defective in hyphae formation, and its response to serum and increased temperature is almost identical to the response of a wild-type strain grown at 37°C in the absence of serum. Thus Efg1p and Cph1p are needed for the activation of the transcriptional program that is induced by the presence of serum.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Article
Authors:Nantel, André and Dignard, Daniel and Bachewich, Catherine and Harcus, Doreen and Marcil, Anne and Bouin, Anne-Pascale and Sensen, Christoph W. and Hogues, Hervé and van het Hoog, Marco and Gordon, Paul and Rigby, Tracey and Benoit, Francois and Tessier, Daniel C. and Thomas, David Y. and Whiteway, Malcolm
Journal or Publication:Molecular Biology of the Cell
Date:October 2002
ID Code:7584
Deposited On:11 May 2011 17:41
Last Modified:11 May 2011 17:41
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