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Sphaeridiotrema globulus and Sphaeridiotrema pseudoglobulus (Digenea): Species Differentiation Based On mtDNA (Barcode) and Partial LSU–rDNA Sequences

Title:

Sphaeridiotrema globulus and Sphaeridiotrema pseudoglobulus (Digenea): Species Differentiation Based On mtDNA (Barcode) and Partial LSU–rDNA Sequences

Bergmame, Laura, Huffman, Jane, Cole, Rebecca, Dayanandan, Selvadurai, Tkach, Vasyl and McLaughlin, J. Daniel (2011) Sphaeridiotrema globulus and Sphaeridiotrema pseudoglobulus (Digenea): Species Differentiation Based On mtDNA (Barcode) and Partial LSU–rDNA Sequences. Journal of Parasitology, 97 (6). pp. 1132-1136. ISSN 0022-3395

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Official URL: http://dx.doi.org/10.1645/GE-2370.1

Abstract

Flukes belonging to Sphaeridiotrema are important parasites of waterfowl, and 2 morphologically similar species Sphaeridiotrema globulus and Sphaeridiotrema pseudoglobulus, have been implicated in waterfowl mortality in North America. Cytochrome oxidase I (barcode region) and partial LSU-rDNA sequences from specimens of S. globulus and S. pseudoglobulus, obtained from naturally and experimentally infected hosts from New Jersey and Quebec, respectively, confirmed that these species were distinct. Barcode sequences of the 2 species differed at 92 of 590 nucleotide positions (15.6%%) and the translated sequences differed by 13 amino acid residues. Partial LSU-rDNA sequences differed at 29 of 1,208 nucleotide positions (2.4%%). Additional barcode sequences from specimens collected from waterfowl in Wisconsin and Minnesota and morphometric data obtained from specimens acquired along the north shore of Lake Superior revealed the presence of S. pseudoglobulus in these areas. Although morphometric data suggested the presence of S. globulus in the Lake Superior sample, it was not found among the specimens sequenced from Wisconsin or Minnesota.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Article
Refereed:Yes
Authors:Bergmame, Laura and Huffman, Jane and Cole, Rebecca and Dayanandan, Selvadurai and Tkach, Vasyl and McLaughlin, J. Daniel
Journal or Publication:Journal of Parasitology
Date:2011
Digital Object Identifier (DOI):10.1645/GE-2370.1
ID Code:973638
Deposited By: ANDREA MURRAY
Deposited On:09 Mar 2012 17:33
Last Modified:18 Jan 2018 17:36
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