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Identification of novel enzymes to enhance the ruminal digestion of barley straw

Title:

Identification of novel enzymes to enhance the ruminal digestion of barley straw

Badhan, Ajay, Ribeiro, Gabriel O., Jones, Darryl R., Wang, Yuxi, Abbott, D. Wade, Di Falco, Marcos, Tsang, Adrian and McAllister, Tim A. (2018) Identification of novel enzymes to enhance the ruminal digestion of barley straw. Bioresource Technology . ISSN 09608524 (In Press)

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Official URL: http://dx.doi.org/10.1016/j.biortech.2018.03.086

Abstract

Crude enzyme extracts typically contain a broad spectrum of enzyme activities, most of which are redundant to those naturally produced by the rumen microbiome. Identification of enzyme activities that are synergistic to those produced by the rumen microbiome could enable formulation of enzyme cocktails that improve fiber digestion in ruminants. Compared to untreated barley straw, Viscozyme® increased gas production, dry matter digestion (P<0.01) and volatile fatty acid production (P<0.001) in ruminal batch cultures. Fractionation of Viscozyme® by Blue Native PAGE and analyses using a microassay and mass-spectrometry revealed a GH74 endoglucanase, GH71 α-1,3-glucanase, GH5 mannanase, GH7 cellobiohydrolase, GH28 pectinase, and esterases from Viscozyme® contributed to enhanced saccharification of barley straw by rumen mix enzymes. Grouping of these identified activities with their carbohydrate active enzymes (CAZy) counterparts enabled selection of similar CAZymes for downstream production and screening. Mining of these specific activities from other biological systems could lead to high value enzyme formulations for ruminants.

Divisions:Concordia University > Research Units > Centre for Structural and Functional Genomics
Item Type:Article
Refereed:Yes
Authors:Badhan, Ajay and Ribeiro, Gabriel O. and Jones, Darryl R. and Wang, Yuxi and Abbott, D. Wade and Di Falco, Marcos and Tsang, Adrian and McAllister, Tim A.
Journal or Publication:Bioresource Technology
Date:28 March 2018
Funders:
  • Alberta Agriculture and Forestry
  • Agriculture and Agri-Food Canada Agri-Innovation Program
Digital Object Identifier (DOI):10.1016/j.biortech.2018.03.086
Keywords:Carbohydrate active enzymes (CAZymes); rumen nutrition; fiber utilization; Blue Native PAGE; LC-MS/MS; Glycosyl hydrolase
ID Code:983646
Deposited By: MONIQUE LANE
Deposited On:29 Mar 2018 19:10
Last Modified:29 Mar 2018 19:10

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