Abstract

An enzyme activity database (EAMDB) is required to support a fungal genomics project currently underway at Concordia. EAMDB supports high-throughput assays to determine enzyme activities, kinetic constants, optimal temperatures and pH values. This thesis implements and validates the design of EAMDB done by Ronghua Shu, a previous Masters student at Concordia. By working closely with the wet lab scientists, we have validated it through several techniques and implemented a MySQL relational database for EAMDB. We also have created the user interfaces on the web for EAMDB and links between protocol files and relations in this database by using PHP Hypertext Preprocessor (PHP)