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Self-assembly and sensor response of photosynthetic reaction centers on screen-printed electrodes


Self-assembly and sensor response of photosynthetic reaction centers on screen-printed electrodes

Bhalla, Vijayender and Zazubovich, Valter (2011) Self-assembly and sensor response of photosynthetic reaction centers on screen-printed electrodes. Analytica Chimica Acta, 707 (1-2). pp. 184-190. ISSN 00032670

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Official URL: http://dx.doi.org/10.1016/j.aca.2011.09.020


Photosynthetic reaction centers were immobilized onto gold screen-printed electrodes (Au-SPEs) using a self-assembled monolayer (SAM) of mercaptopropionic acid (MPA) which was deliberately defective in order to achieve effective mediator transfer to the electrodes. The pure Photosystem II (PS II) cores from spinach immobilize onto the electrodes very efficiently but fair badly in terms of photocurrent response (measured using duroquinone as the redox mediator). The cruder preparation of PS II known as BBY particles performs significantly better under the same experimental conditions and shows a photocurrent response of 20–35 nA (depending on preparation) per screen-printed electrode surface (12.5 mm2). The data was corroborated using AFM, showing that in the case of BBY particles a defective biolayer is indeed formed, with grooves spanning the whole thickness of the layer enhancing the possibility of mass transfer to the electrodes and enabling biosensing. In comparison, the PS II core layer showed ultra-dense organization, with additional formation of aggregates on top of the single protein layer, thus blocking mediator access to the electrodes and/or binding sites. The defective monolayer biosensor with BBY particles was successfully applied for the detection of photosynthesis inhibitors, demonstrating that the inhibitor binding site remained accessible to both the inhibitor and the external redox mediator. Biosensing was demonstrated using picric acid and atrazine. The detection limits were 1.15 nM for atrazine and 157 nM for picric acid.

Divisions:Concordia University > Faculty of Arts and Science > Physics
Item Type:Article
Authors:Bhalla, Vijayender and Zazubovich, Valter
Journal or Publication:Analytica Chimica Acta
Date:30 November 2011
Digital Object Identifier (DOI):10.1016/j.aca.2011.09.020
Keywords:Photosynthetic reaction centers; Gold screen printed electrode; Self-assembled monolayer; Herbicide detection; Atrazine; Picric acid
ID Code:36039
Deposited By: Danielle Dennie
Deposited On:28 Oct 2011 17:21
Last Modified:18 Jan 2018 17:36


[1] Z. Dai and H. Ju. Phys. Chem. Chem. Phys., 3 (2001), pp. 3769–3773.

[2] N.K. Chaki and K. Vijayamohanan. Biosens. Bioelectron., 17 (2002), pp. 1–12.

[3] S. Ferretti, S. Paynter, D.A. Russell, K.E. Sapsford and D.J. Richardson. Trends Anal. Chem., 19 (2000), pp. 530–540.

[4] F. Frederix, K. Bonroy, W. Laureyn, G. Reekmans, A. Campitelli, W. Dehaen and G. Maes. Langmuir, 19 (2003), pp. 4351–4357.

[5] C.R. Suri, R. Boro, Y. Nangia, S. Gandhi, P. Sharma, N. Wangoo, K. Rajesh and G.S. Shekhawat. Trends Anal. Chem., 28 (2009), pp. 29–39.

[6] B. Byrne, E. Stack, N. Gilmartin and R.O. Kennedy. Sensors, 9 (2009), pp. 4407–4445.

[7] I. Shitanda, S. Takamatsu, K. Watanabe and M. Itagaki. Electrochim. Acta, 54 (2009), pp. 4933–4936.

[8] H. Peters, C.S. Dannert and R.D. Schmid. Mater. Sci. Eng. C, 4 (1997), pp. 227–232.

[9] S. Lemieux and R. Carpentier. J. Photochem. Photobiol. B, 2 (1988), pp. 221–231.

[10] S. Lemieux and R. Carpentier. Photochem. Photobiol., 48 (1988), pp. 115–121.

[11] C. Loranger and R. Carpentier. Biotechnol. Bioeng., 44 (1994), pp. 178–183.

[12] D. Laberge, J. Chartrand, R. Rouillon and R. Carpentier. Environ. Toxicol. Chem., 18 (1999), pp. 2851–2858.

[13] M. Koblízek, J. Masojídek, J. Komenda, T. Kucera, R. Pilloton, A.K. Mattoo and M.T. Giardi. Biotechnol. Bioeng., 60 (1998), pp. 664–669.

[14] F. Bettazzi, L. Laschi and M. Mascini. Anal. Chim. Acta, 589 (2007), pp. 14–21.

[15] E. Touloupakis, L. Giannoudi, S.A. Piletsky, L. Guzzella, F. Pozzoni and M.T. Giardi. Biosens. Bioelectron., 20 (2005), pp. 1984–1992.

[16] M. Koblízek, J. Maly, J. Masojídek, J. Komenda, T. Kucera, M.T. Giardi, A.K. Mattoo and R. Pilloton. Biotechnol. Bioeng., 78 (2002), pp. 110–116.

[17] A. Tibuzzi, G. Pezzotti, T. Lavecchia, G. Rea and M.T. Giardi. Sens. Transducers, 88 (2008), p. 9.

[18] K. Buonasera, G. Pezzotti, V. Scognamiglio, A. Tibuzzi and M.T. Giardi. J. Agric. Food Chem., 58 (2010), pp. 5982–5990.

[19] E.V. Piletskaya, S.A. Piletsky, T.A. Sergeyeva, A.V. El'skaya, A.A. Sozinov, J.L. Marty and R. Rouillon. Anal. Chim. Acta, 391 (1999), pp. 1–7.

[20] K.B. Lam, E.F. Irwin, K.E. Healy and L. Lin. Sens. Actuators B: Chem., 117 (2006), pp. 480–487.

[21] N. Terasaki, M. Iwai, N. Yamamoto, T. Hiraga, S. Yamada and Y. Inoue. Thin Solid Films, 516 (2008), pp. 2553–2557.

[22] J. Maly, J. Krejci, M. Ilie, L. Jakubka, J. Masojidek, R. Pilloton, K. Sameh, P. Steffan, Z. Stryhal and M. Sugiura. Anal. Bioanal. Chem., 381 (2005), pp. 1558–1567.

[23] S. Campuzano, M. Pedrero, C. Montemayor, E. Fatas and J.M. Pingarron. J. Electroanal. Chem., 586 (2006), pp. 112–121.

[24] D.A. Berthold, G.T. Babcock and C.F. Yocum. FEBS Lett., 134 (1981), p. 231.

[25] D.I. Arnon. Plant Physiol., 24 (1949), pp. 1–15.

[26] P.J. van Leeuwen, M.C. Nieveen, E.J. van de Meent, J.P. Dekker and H.J. Gorkom. Photosynth. Res., 28 (1991), pp. 149–153.

[27] T. Hermanson, Bioconjugate Techniques, Academic Press, San Diego, CA (1996), pp. 139–140.

[28] V. Bhalla, X. Zhao and V. Zazubovich. J. Electoanal. Chem., 657 (2011), pp. 84–90.

[29] J. Shen and C.C. Liu. Sens. Actuators B: Chem., 120 (2007), pp. 417–425.

[30] O.A. Loaiza, S. Campuzano, M. Pedrero and J.M. Pingarron. Electroanalysis, 20 (2008), pp. 1397–1405.

[31] C.D. Bain, E.B. Troughton, Y.Y. Tao, J. Evall, G.M. Whitesides and R.G. Nuzzo. J. Am. Chem. Soc., 111 (1989), pp. 321–335.

[32] V. Anandan, R. Gangadharan and G. Zhang. Sensors, 9 (2009), pp. 1295–1305.

[33] V. Bhalla, S. Carrara, C. Stagni and B. Samorì. Thin Solid Films, 518 (2010), pp. 3360–3366.

[34] K. Alcantara, B. Munge, Z. Pendon, H.A. Frank and J.F. Rusling. J. Am. Chem. Soc., 128 (2006), pp. 14930–14937.

[35] J. Maly, A. Masci, J. Masojidek, M. Sugiura and R. Pilloton. Anal. Lett., 37 (2004), pp. 1645–1656.

[36] S. Carrara, V. Bhalla, C. Stagni and B. Samorì. Surf. Sci., 603 (2009), pp. 75–77.

[37] E.J. Boekma, B. Hankamer, D. Bold, J. Kruip, J. Nield, A.F. Boonstra, J. Barber and M. Rogner. Proc. Natl. Acad. Sci. U.S.A., 92 (1995), pp. 175–179.

[38] A. Morrin, A. Guzman, A.J. Killard, J.M. Pingarron and M.R. Smyth. Biosens. Bioelectron., 18 (2003), pp. 715–720.

[39] M. Vittadello, M.Y. Gorbunov, D.T. Mastrogiovanni, L.S. Wielunski, E.L. Garfunkel, F. Guerrero, D. Kirilovsky, M. Sugiura, A.W. Rutherford, A. Safari and P.G. Falkowski. ChemSusChem, 3 (2010), pp. 474–475.

[40] R. Ganapathy, S. Manolache, M. Sarmadi, W.J. Simonsick and F. Denes. J. Appl. Polym. Sci., 78 (2000), pp. 1783–1796.

[41] Z. Naal, J.H. Park, S. Bernhard, J.P. Shapleigh, C.A. Batt and H.D. Abrua. Anal. Chem., 74 (2002), pp. 140–148.

[42] R.D. Richins, A. Mulchandi and W. Chen. Biotechnol. Bioeng., 69 (2000), pp. 591–596.

[43] M. Darder, E. Casero, F. Pariente and E. Lorenzo. Anal. Chem., 72 (2000), pp. 3784–3792.

[44] W. Oettmeier and K. Masson. Eur. J. Biochem., 122 (1982), pp. 163–167.

[45] J. Lu and Z. Zhang. Anal. Chim. Acta, 318 (1996), p. 175. Article | PDF (385 K)

[46] G. He, H. Peng, T. Liu, M. Yang, Y. Zhang and Y. Fang. J. Mater. Chem., 19 (2009), p. 7347.

[47] S. Hleli, C. Martelet, A. Abdelghani, N. Burais and N. Jaffrezic-Renault. Sens. Actuators B, 113 (2006), pp. 711–717.

[48] E. Valera, J. Ramón-Azcón, F.-J. Sanchez, M.-P. Marco and Á. Rodríguez. Sens. Actuators B, 134 (2008), pp. 95–103.

[49] J. Přibyl, M. Hepel, J. Halamek and P. Skladal. Sens. Actuators B, 91 (2003), pp. 333–341.

[50] C. Steegborn and P. Skladal. Biosens. Bioelectron., 12 (1997), pp. 19–27.

[51] C.R. Suri, J. Kaur, S. Gandhi and G.S. Shekhawat. Nanotechnology, 19 (2008), p. 235502.

[52] R. Wilson, M.H. Barker, D.J. Schiffrin and R. Abuknesha. Biosens. Bioelectron., 12 (1997), pp. 277–286.

[53] J. Kaur, R.C. Boro, N. Wangoo, K. Rajesh and C.R. Suri. Anal. Chim. Acta, 607 (2008), pp. 92–98.
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