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Dynamic ergosterol- and ceramide-rich domains in the peroxisomal membrane serve as an organizing platform for peroxisome fusion


Dynamic ergosterol- and ceramide-rich domains in the peroxisomal membrane serve as an organizing platform for peroxisome fusion

Boukh-Viner, Tatiana, Guo, Tong, Alexandrian, Alex, Cerracchio, André, Gregg, Christopher, Haile, Sandra, Kyskan, Robert, Milijevic, Svetlana, Oren, Daniel, Solomon, Jonathan, Wong, Vivianne, Nicaud, Jean-Marc, Rachubinski, Richard A., English, Ann M. ORCID: https://orcid.org/0000-0002-3696-7710 and Titorenko, Vladimir I. ORCID: https://orcid.org/0000-0001-5819-7545 (2005) Dynamic ergosterol- and ceramide-rich domains in the peroxisomal membrane serve as an organizing platform for peroxisome fusion. The Journal of Cell Biology, 168 (5). pp. 761-773. ISSN 0021-9525

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Official URL: http://dx.doi.org/10.1083/jcb.200409045


We describe unusual ergosterol- and ceramide-rich (ECR) domains in the membrane of yeast peroxisomes. Several key features of these detergent-resistant domains, including the nature of their sphingolipid constituent and its unusual distribution across the membrane bilayer, clearly distinguish them from well characterized detergent-insoluble lipid rafts in the plasma membrane. A distinct set of peroxisomal proteins, including two ATPases, Pex1p and Pex6p, as well as phosphoinositide- and GTP-binding proteins, transiently associates with the cytosolic face of ECR domains. All of these proteins are essential for the fusion of the immature peroxisomal vesicles P1 and P2, the earliest intermediates in a multistep pathway leading to the formation of mature, metabolically active peroxisomes. Peroxisome fusion depends on the lateral movement of Pex1p, Pex6p, and phosphatidylinositol-4,5-bisphosphate–binding proteins from ECR domains to a detergent-soluble portion of the membrane, followed by their release to the cytosol. Our data suggest a model for the multistep reorganization of the multicomponent peroxisome fusion machinery that transiently associates with ECR domains.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Article
Authors:Boukh-Viner, Tatiana and Guo, Tong and Alexandrian, Alex and Cerracchio, André and Gregg, Christopher and Haile, Sandra and Kyskan, Robert and Milijevic, Svetlana and Oren, Daniel and Solomon, Jonathan and Wong, Vivianne and Nicaud, Jean-Marc and Rachubinski, Richard A. and English, Ann M. and Titorenko, Vladimir I.
Journal or Publication:The Journal of Cell Biology
Date:28 February 2005
Digital Object Identifier (DOI):10.1083/jcb.200409045
ID Code:7562
Deposited By: Danielle Dennie
Deposited On:11 May 2011 16:37
Last Modified:29 May 2019 12:48


Bagnat, M., S. Keranen, A. Shevchenko, A. Shevchenko, and K. Simons. 2000. Lipid rafts function in biosynthetic delivery of proteins to the cell surface in yeast. Proc. Natl. Acad. Sci. USA. 97:3254–3259.

Brown, D.A., and J.K. Rose. 1992. Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface. Cell. 68:533–544.

Brown, D.A., and E. London. 2000. Structure and function of sphingolipid- and cholesterol-rich membrane rafts. J. Biol. Chem. 275:17221–17224.

Eckert, J.H., and R. Erdmann. 2003. Peroxisome biogenesis. Rev. Physiol. Biochem. Pharmacol. 147:75–121.

Foster, L.J., C.L. de Hoog, and M. Mann. 2003. Unbiased quantitative proteomics of lipid rafts reveals high specificity for signaling factors. Proc. Natl. Acad. Sci. USA. 100:5813–5818.

Guo, T., Y.Y. Kit, J.M. Nicaud, M.T. Le Dall, S.K. Sears, H. Vali, H. Chan, R.A. Rachubinski, and V.I. Titorenko. 2003. Peroxisome division is regulated by a signal from inside the peroxisome. J. Cell Biol. 162:1255–1266.

Helms, J.B., and C. Zurzolo. 2004. Lipids as targeting signals: lipid rafts and intracellular trafficking. Traffic. 5:247–254.

Jahn, R., T. Lang, and T.C. Südhof. 2003. Membrane fusion. Cell. 112:519–533.

Kenworthy, A.K., B.J. Nichols, C.L. Remmert, G.M. Hendrix, M. Kumar, J. Zimmerberg, and J. Lippincott-Schwartz. 2004. Dynamics of putative raft-associated proteins at the cell surface. J. Cell Biol. 165:735–746.

Mañes, S., G. del Real, and C. Martínez-A. 2003. Pathogens: raft hijackers. Nat. Rev. Immunol. 3:557–568.

Mayer, A. 2002. Membrane fusion in eukaryotic cells. Annu. Rev. Cell Dev. Biol. 18:289–314.

Mayor, S., and M. Rao. 2004. Rafts: scale-dependent, active lipid organization at the cell surface. Traffic. 5:231–240.

Mayor, S., and H. Riezman. 2004. Sorting GPI-anchored proteins. Nat. Rev. Mol. Cell Biol. 5:110–120.

Mozdy, A.D., and J.M. Shaw. 2003. A fuzzy mitochondrial fusion apparatus comes into focus. Nat. Rev. Mol. Cell Biol. 4:468–478.

Munro, S. 2003. Lipid rafts: elusive or illusive? Cell. 115:377–388.

Pierini, L.M., and F.R. Maxfield. 2001. Flotillas of lipid rafts fore and aft. Proc. Natl. Acad. Sci. USA. 98:9471–9473.

Pomorski, T., J.C. Holthuis, A. Herrmann, and G. van Meer. 2004. Tracking down lipid flippases and their biological functions. J. Cell Sci. 117:805–813.

Purdue, P.E., and P.B. Lazarow. 2001. Peroxisome biogenesis. Annu. Rev. Cell Dev. Biol. 17:701–752.

Röper, K., D. Corbeil, and W.B. Huttner. 2000. Retention of prominin in microvilli reveals distinct cholesterol-based lipid micro-domains in the apical plasma membrane. Nat. Cell Biol. 2:582–592.

Salaün, C., D.J. James, and L.H. Chamberlain. 2004. Lipid rafts and the regulation of exocytosis. Traffic. 5:255–264.

Simons, K., and D. Toomre. 2000. Lipid rafts and signal transduction. Nat. Rev. Mol. Cell Biol. 1:31–39.

Slimane, T.A., G. Trugnan, S.C. van IJzendoorn, and D. Hoekstra. 2003. Raft-mediated trafficking of apical resident proteins occurs in both direct and transcytotic pathways in polarized hepatic cells: role of distinct lipid microdomains. Mol. Biol Cell. 14:611–624.

Sprong, H., P. van der Sluijs, and G. van Meer. 2001. How proteins move lipids and lipids move proteins. Nat. Rev. Mol. Cell Biol. 2:504–513.

Subramani, S., A. Koller, and W.B. Snyder. 2000. Import of peroxisomal matrix and membrane proteins. Annu. Rev. Biochem. 69:399–418.

Titorenko, V.I., and R.A. Rachubinski. 2000. Peroxisomal membrane fusion requires two AAA family ATPases, Pex1p and Pex6p. J. Cell Biol. 150:881–886.

Titorenko, V.I., and R.A. Rachubinski. 2001. Dynamics of peroxisome assembly and function. Trends Cell Biol. 11:22–29.

Titorenko, V.I., J.J. Smith, R.K. Szilard, and R.A. Rachubinski. 1998. Pex20p of the yeast Yarrowia lipolytica is required for the oligomerization of thiolase in the cytosol and for its targeting to the peroxisome. J. Cell Biol. 142:403–420.

Titorenko, V.I., H. Chan, and R.A. Rachubinski. 2000. Fusion of small peroxisomal vesicles in vitro reconstructs an early step in the in vivo multistep peroxisome assembly pathway of Yarrowia lipolytica. J. Cell Biol. 148:29–43.

Wagner, P., L. Hengst, and D. Gallwitz. 1992. Ypt proteins in yeast. Methods Enzymol. 219:369–387.

Xu, X., R. Bittman, G. Duportail, D. Heissler, C. Vilcheze, and E. London. 2001. Effect of the structure of natural sterols and sphingolipids on the formation of ordered sphingolipid/sterol domains (rafts). Comparison of cholesterol to plant, fungal, and disease-associated sterols and comparison of sphingomyelin, cerebrosides, and ceramide. J. Biol. Chem. 276:33540–33546.

Zinser, E., C.D. Sperka-Gottlieb, E.V. Fasch, S.D. Kohlwein, F. Paltauf, and G. Daum. 1991. Phospholipid synthesis and lipid composition of subcellular membranes in the unicellular eukaryote Saccharomyces cerevisiae. J. Bacteriol. 173:2026–2034.
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