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Stress induces the assembly of RNA granules in the chloroplast of Chlamydomonas reinhardtii


Stress induces the assembly of RNA granules in the chloroplast of Chlamydomonas reinhardtii

Uniacke, James and Zerges, William (2008) Stress induces the assembly of RNA granules in the chloroplast of Chlamydomonas reinhardtii. Journal of Cell Biology, 182 (4). pp. 641-646. ISSN 1540-8140

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Official URL: http://dx.doi.org/10.1083/jcb.200805125


Eukaryotic cells under stress repress translation and localize these messenger RNAs (mRNAs) to cytoplasmic RNA granules. We show that specific stress stimuli induce the assembly of RNA granules in an organelle with bacterial ancestry, the chloroplast of Chlamydomonas reinhardtii. These chloroplast stress granules (cpSGs) form during oxidative stress and disassemble during recovery from stress. Like mammalian stress granules, cpSGs contain poly(A)-binding protein and the small, but not the large, ribosomal subunit. In addition, mRNAs are in continuous flux between polysomes and cpSGs during stress. Localization of cpSGs within the pyrenoid reveals that this chloroplast compartment functions in this stress response. The large subunit of ribulosebisphosphate carboxylase/oxygenase also assembles into cpSGs and is known to bind mRNAs during oxidative stress, raising the possibility that it plays a role in cpSG assembly. This discovery within such an organelle suggests that mRNA localization to granules during stress is a more general phenomenon than currently realized.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Article
Authors:Uniacke, James and Zerges, William
Journal or Publication:Journal of Cell Biology
Date:18 August 2008
Digital Object Identifier (DOI):10.1083/jcb.200805125
ID Code:7605
Deposited By: Danielle Dennie
Deposited On:16 May 2011 15:35
Last Modified:18 Jan 2018 17:31


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