Zerges, William and Rochaix, Jean-David (1998) Low Density Membranes Are Associated with RNA-binding Proteins and Thylakoids in the Chloroplast of Chlamydomonas reinhardtii. The Journal of Cell Biology, 140 (1). pp. 101-110. ISSN 00219525 (In Press)
Preview |
Text (application/pdf)
357kBZerges_JCB1998.pdf - Published Version |
Official URL: http://dx.doi.org/10.1083/jcb.140.1.101
Abstract
Chloroplast subfractions were tested with a UV cross-linking assay for proteins that bind to the 5′ untranslated region of the chloroplast psbC mRNA of the green alga Chlamydomonas reinhardtii. These analyses revealed that RNA-binding proteins of 30–32, 46, 47, 60, and 80 kD are associated with chloroplast membranes. The buoyant density and the acyl lipid composition of these membranes are compatible with their origin being the inner chloroplast envelope membrane. However, unlike previously characterized inner envelope membranes, these membranes are associated with thylakoids. One of the membrane-associated RNA-binding proteins appears to be RB47, which has been reported to be a specific activator of psbA mRNA translation. These results suggest that translation of chloroplast mRNAs encoding thylakoid proteins occurs at either a subfraction of the chloroplast inner envelope membrane or a previously uncharacterized intra-chloroplast compartment, which is physically associated with thylakoids.
| Divisions: | Concordia University > Faculty of Arts and Science > Biology |
|---|---|
| Item Type: | Article |
| Refereed: | Yes |
| Authors: | Zerges, William and Rochaix, Jean-David |
| Journal or Publication: | The Journal of Cell Biology |
| Date: | 12 January 1998 |
| Digital Object Identifier (DOI): | 10.1083/jcb.140.1.101 |
| ID Code: | 7607 |
| Deposited By: | Danielle Dennie |
| Deposited On: | 16 May 2011 15:51 |
| Last Modified: | 18 Jan 2018 17:31 |
References:
Andreasson, E, P Svensson, C Weibull, P-A Albertsson(1988) Separation and characterization of stromal and grana membranes -evidence for heterogeneity in antenna size of both photosystem I and photosystem II. Biochim Biophys Acta 936:339–350.Barber, J(1980) Membrane surface charges and potentials in relation to photosynthesis. Biochim Biophys Acta 594:253–308, pmid:7018576.
Block, M.A., A-J. Dorne, J. Joyard, and R. Douce. 1983. Preparation and characterization of membrane fractions enriched in outer and inner envelope membranes from spinach chloroplasts. J. Biol. Chem. 258. 13273–13280.
Chen, Q, CC Adams, L Usack, J Yang, R-A Monde, DB Stern(1995) An AU-rich element in the 3′ untranslated region of the spinach chloroplast petDgene participates in sequence-specific RNA-protein complex formation. Mol Cell Biol 15:2010–2018, pmid:7891696.
Clemetson, JM, A Boschetti, KJ Clemetson(1992) Chloroplast envelope proteins are encoded by the chloroplast genome of Chlamydomonas reinhardtii. J Biol Chem 267:19773–19779, pmid:1400290.
Cline, K, R Henry(1996) Import and routing of nucleus-encoded chloroplast proteins. Annu Rev Cell Dev Biol 12:1–26, pmid:8970720.
Dake, E, TJ Hofmann, S McIntire, A Hudson, H Zassenhaus(1988) Purification and properties of the major nuclease from mitochondria of Saccharomyces cerevisiae. J Biol Chem 263:7691–7702, pmid:3286639.
Danon, A, SP Mayfield(1991) Light regulated translational activators: identification of chloroplatic gene specific mRNA binding proteins. EMBO (Eur Mol Biol Organ) J 10:3993–4001, pmid:1721866.
Danon, A, SP Mayfield(1994a) ADP-dependent phosphorylation regulates RNA-binding in vitro: implications in light-modulated translation. EMBO (Eur Mol Biol Organ) J 13:2227–2235, pmid:8187775.
Danon, A, SP Mayfield(1994b) Light-regulated translation of chloroplast messenger RNAs through redox potential. Science 266:1717–1719, pmid:7992056.
Davies, DR, A Plaskitt(1971) Genetical and structural analyses of cell-wall formation in Chlamydomonas reinhardtii. Genet Res 17:33–43.
Douce, R, RB Holtz, AA Benson(1973) Isolation and properties of the envelope of Spinach chloroplasts. J Biol Chem 248:7215–7222, pmid:4147558.
Durrant, I(1990) Light-based detection of biomolecules. Nature 346:297–298, pmid:1695714.
Fox, T.D. 1996. Genetics of mitochondrial translation. In Translational Control. J.W.B. Hershey, M.B. Matthews, and N. Sonenberg, editor. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 733–758.
Fujiki, Y, AL Hubbard, S Fowler, PB Lazrow(1982) Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum. J Cell Biol 93:97–102, pmid:7068762.
Ghanotakis, DF, CF Yocum(1990) Photosystem II and the oxygen-evolving complex. Annu Rev Plant Physiol Plant Mol Biol 41:255–276.
Gillham, NW, JE Boynton, CR Hauser(1994) Translational regulation of gene expression in chloroplasts and mitochondria. Annu Rev Genet 28:71–93, pmid:7893142.
Goldberg, I, I Ohad(1970) Biogenesis of chloroplast membranes: IV. Lipid and pigment changes during synthesis of chloroplast membranes in a mutant of Chlamydomonas reinhardtii y-1. J Cell Biol 44:563–571, pmid:5415237.
Gorman, DS, RP Levine(1965) Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 54:1665–1669, pmid:4379719.
Gruenberg, J, MJ Clague(1992) Regulation of intracellular membrane transport. Curr Opin Cell Biol 4:593–599, pmid:1419040.
Harris, EH, JE Boynton, N W Gillham(1994) Chloroplast ribosomes and protein synthesis. Microbiol Rev 58:700–754, pmid:7854253.
Hashimoto, A, WF Ettinger, Y Yamamoto, S M Theg(1997) Assembly of newly imported oxygen-evolving complex subunits in isolated chloroplasts: sites of assembly and mechanism of binding. Plant Cell 9:441–452, pmid:12237359.
Hatefi, Y, WG Hanstien(1974) Destabilization of membranes with chaotropic ions. Methods Enzymol 31:770–790, pmid:4370616.
Hauser, CR, NW Gillham, J E Boynton(1996) Translational regulation of chloroplast genes. J Biol Chem 271:1486–1497, pmid:8576143.
Herrin, D, A Michaels, E Hickey(1981) Synthesis of a chloroplast membrane polypeptides on thylakoid-bound ribosomes during the cell cycle of Chlamydomonas reinhardtii. Biochim Biophys Acta 655:136–145.
Hinnebusch, A.G., and S.W. Liebman. 1991. Protein synthesis and translational control in Saccharomyces. In The Molecular and Cellular Biology of the Yeast Saccharomyces: Genome Dynamics, Protein Synthesis, and Energetics, Vol. 1. J.R. Broach, E.W. Jones and J.R. Pringle, editors. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 627–735.
Hoober, JK, CO Boyd, L Paavola(1991) Origin of thylakoid membranes in Chlamydomonas reinhardtii y-1at 38°C. Plant Physiol 96:1321–1328, pmid:16668337.
Hsu-Ching, C, DB Stern(1991) Specific binding of chloroplast proteins in vitro to the 3′ untranslated region of spinach chloroplast petD mRNA. Mol Cell Biol 11:4380–4388, pmid:1908552.
Hugueney, P, F Bouvier, A Badillo, A D'Harlingue, M Kuntz, B Camara(1995) Identification of a plastid protein involved in vesicle fusion and/ or membrane protein translocation. Proc Natl Acad Sci USA 92:5630–5634, pmid:7777561.
Joyard, J, MA Block, R Douce(1991) Molecular aspects of plastid envelope biochemistry. Eur J Biochem 199:489–509, pmid:1868841.
Michaelis, U, A Körte, G Rödel(1991) Association of cytochrome btranslational activator proteins with the mitochondrial membrane: implications for cytochrome b expression in yeast. Mol Gen Genet 230:177–185, pmid:1745228.
McMulin, TW, TD Fox(1993) COX3 mRNA-specific translational activator proteins are associated with the inner mitochondrial membrane in Saccharomyces cerevisiae. J Biol Chem 268:11737–11741, pmid:8389363.
Morré, D.J., Selldén, G., C. Sundqvist, and A.S. Sandelius. 1991. Stromal low temperature compartment derived from the inner membrane of the chloroplast envelope. Plant Physiol. 97:1558–1564.
Nickelsen, J, J van Dillewijn, M Rahire, J-D Rochaix(1994) Determinants for stability of the chloroplast psbD mRNA are located within its short leader region in Chlamydomonas reinhardtii. EMBO (Eur Mol Biol Organ) J 13:3182–3191, pmid:8039511.
Porra, RJ, WA Thompson, PE Kriedemann(1989) Determination of accurate extinction coefficients and simultaneous equations for assaying the concentration of chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta 975:384–394.
Rawyler, A, M Meylan-Bettex, P-A Siegenthaler(1995) (Galacto)lipid export from envelope to thylakoid membranes in intact chloroplasts. II. A general process with a key role for the envelope in the establishment of lipid asymmetry in thylakoid membranes. Biochim Biophys Acta 1233:123–133, pmid:7865537.
Reinbothe, S, C Reinbothe(1996) The regulation of enzymes involved in chlorophyll biosynthesis. Eur J Biochem 237:323–343, pmid:8647070.
Rochaix, J-D(1996) Post-transcriptional regulation of chloroplast gene expression in Chlamydomonas reinhardtii. Plant Mol Biol 32:327–341, pmid:8980486.
Ryu, EK, M MacCoss(1979) Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms. J Lipid Res 20:561–563, pmid:458272.
Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Second edition. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 18.60–18.74.
Sato, N, C Albrieux, J Joyard, R Douce, T Kuroiwa(1993) Detection and characterization of plastid envelope DNA-binding protein which may anchor plastid nucleoids. EMBO (Eur Mol Biol Organ) J 12:555–561, pmid:8440245.
Smith, PK, RI Krohn, GT Hermanson, AK Mallia, FH Gartner, MD Provenzano, EK Fujimoto, NM Goeke, BJ Olson, DC Klenk(1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150:76–85, pmid:3843705.
Wersel, D, UI Flügge(1984) A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Anal Biochem 138:141–143, pmid:6731838.
Wiesenberger, G, TD Fox(1997) Pet127p, a membrane-associated protein involved in stability and processing of Saccharomyces cerevisiaemitochondrial RNAs. Mol Cell Biol 17:2816–2824, pmid:9111353.
Yohn, CB, A Cohen, A Danon, SP Mayfield(1996) Altered mRNA binding activity and decreased translation initiation in a nuclear mutant lacking translation of the chloroplast psbAmRNA. Mol Cell Biol 16:3560–3566, pmid:8668172.
Zerges, W, J-D Rochaix(1994) The 5′ leader of a chloroplast mRNA mediates the translational requirements for two nucleus-encoded functions in Chlamydomonas reinhardtii. Mol Cell Biol 14:5268–5277, pmid:8035805
All items in Spectrum are protected by copyright, with all rights reserved. The use of items is governed by Spectrum's terms of access.
Repository Staff Only: item control page
Download Statistics
Download Statistics