Bhalla, Vijayender, Zhao, Xin and Zazubovich, Valter (2011) Detection of explosive compounds using Photosystem II-based biosensor. Journal of Electroanalytical Chemistry, 657 (1-2). pp. 84-90. ISSN 15726657
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Official URL: http://dx.doi.org/10.1016/j.jelechem.2011.03.026
Abstract
The efficacy of a Photosystem II (PS II)-based biosensor for the detection of explosive compounds has been explored. The idea is based on the close similarities in the chemical structures of the widespread explosives and herbicides, with the latter known to inhibit functioning of the PS II by attaching to the binding site of the QB mobile plastoquinone electron acceptor. The gold screen-printed electrodes (Au-SPE) functionalized with PS II-enriched particles were used for the detection of explosives in a droplet biosensor configuration. A crude preparation of PS II produced from spinach leaves, known as BBY particles, was employed to modify the Au-SPE working electrode employing BSA–glutaraldehyde-based immobilization procedure. Inhibition of the PS II functioning was detected by photo-electrochemical measurements in the presence of a mediator (either non-native quinone or ferricyanide). The biosensor was highly responsive to herbicides (as expected) as well as to picric acid, with limits of detection in the nanomolar range, but trace detection of trinitrotoluene (TNT) was not effective. The detection limit for picric acid was 25 nM as compared to 400 nM for TNT with duroquinone mediator. Low affinity of PS II to TNT has been corroborated by means of DCPIP assay; possible reasons for low affinity are discussed.
| Divisions: | Concordia University > Faculty of Arts and Science > Physics |
|---|---|
| Item Type: | Article |
| Refereed: | Yes |
| Authors: | Bhalla, Vijayender and Zhao, Xin and Zazubovich, Valter |
| Journal or Publication: | Journal of Electroanalytical Chemistry |
| Date: | 1 July 2011 |
| Digital Object Identifier (DOI): | 10.1016/j.jelechem.2011.03.026 |
| Keywords: | Photosystem II; Herbicides; Picric acid; TNT; Screen-printed electrode |
| ID Code: | 7632 |
| Deposited By: | Danielle Dennie |
| Deposited On: | 03 Jun 2011 18:11 |
| Last Modified: | 18 Jan 2018 17:31 |
References:
[1] J.V. Goodpaster and V.L. McGuffin, Anal. Chem. 73 (2001), p. 2004.[2] K. Albert and J.D.R. Walt, Anal. Chem. 72 (2001), p. 1947.
[3] J.S. Yang and T.M. Swager, J. Am. Chem. Soc. 120 (1998), p. 5321.
[4] A. Rose, Z. Zhu, C.F. Madigan, T.M. Swager and B. Vladimir, Nature 434 (2005), p. 876.
[5] R.D. Shankaran, K.V. Gobi, T. Sakai, K. Matsumoto, K. Toko and N. Miura, Biosens. Bioelectron. 20 (2005), p. 1750.
[6] R. S. Burlage, K. Everman, D. Patek, 1999, US Patent 5972,638.
[7] R. Carpentier, S. Lemieux, M. Mimeault, M. Purcell and D.C. Goetze, Bioelectrochem. Bioenerg. 22 (1989), p. 391.
[8] D. Laberge, J. Chartrand, R. Rouillon and R. Carpentier, Environ. Toxicol. Chem. 18 (1999), p. 2851.
[9] M.T. Giardi, M. Koblízek and J. Masojídek, Biosens. Bioelectron. 16 (2001), p. 1027. Article
[10] M. Koblízek, J. Masojídek, J. Komenda, T. Kucera, R. Pilloton, A.K. Mattoo and M.T. Giardi, Biotech. Bioeng. 60 (1998), p. 664.
[11] M. Koblízek, J. Malý, J. Masojídek, J. Komenda, T. Kucera, M.T. Giardi, A.K. Mattoo and R. Pilloton, Biotech. Bioeng. 78 (2002), p. 110.
[12] J. Maly, A. Masci, J. Masojidek, M. Sugiura and R. Pilloton, Anal. Lett. 37 (2004), p. 1645.
[13] J. Maly, B.J. Maly, C. Di Meo, M. De Francesco, A. Masci, J. Masojidek, M. Sugiura, A. Volpe and R. Pilloton, Bioelectrochemistry 63 (2004), p. 271.
[14] A. Tibuzzi, G. Pezzotti, T. Lavecchia, G. Rea and M.T. Giardi, Sens. Transducers 88 (2008), p. 9.
[15] M. Campàs, R. Carpentier and R. Rouillon, Biotechnol. Adv. 26 (2008), p. 370.
[16] C. Jian and W.R. Seitz, Anal. Chim. Acta 237 (1990), p. 265.
[17] H.H. Zeng, K.M. Wang and R.Q. Yu, Talanta 40 (1993), p. 1569.
[18] C.G. Niu, Z.Z. Li, X.B. Zhang, W.Q. Lin, G.L. Shen and R.Q. Yu, Anal. Bioanal. Chem. 372 (2002), p. 519.
[19] X. Yang, C.G. Niu, G.L. Shen and R.Q. Yu, Analyst 126 (2001), p. 349.
[20] W. Oettmeier and K. Masson, Eur. J. Biochem. 122 (1982), p. 163.
[21] E. Ercag, A. Uzer and R. Apak, Talanta 78 (2009), p. 772.
[22] E. Ercag, A. Uzer and R. Apak, Anal. Chim. Acta 505 (2004), p. 83.
[23] J. Wannlund and M. Deluca, Anal. Biochem. 122 (1982), p. 385.
[24] J. Wang, R.K. Bhada, J. Lu and D. MacDonald, Anal. Chim. Acta 361 (1998), p. 85.
[25] H. Sohn, R.M. Calhoun, M.J. Sailor and W.C. Trogler, Angew. Chem. Int. Ed. 113 (2001), p. 2162.
[26] K.S. Ro, A. Venugopal, D.D. Adrian, D. Constant, K. Qaisi, K.T. Valsaraj, L.J. Thibodeaux and D. Roy, J. Chem. Eng. Data 41 (1996), p. 758.
[27] N.D. Ali, D. Dewez, P.Y. Robidoux and R. Popovic, Ecotoxicology 15 (2006), p. 437.
[28] D.A. Berthold, G.T. Babcock and C.F. Yocum, FEBS Lett. 134 (1981), p. 231.
[29] P.J. van Leeuwen, M.C. Nieveen, E.J. van de Meent, J.P. Dekker and H.J. Gorkom, Photosyn. Res. 28 (1991), pp. 149–153.
[30] D.I. Arnon, Plant Physiol 24 (1949), p. 1.
[31] R. Carpentier and S. Lemieux, Appl. Biochem. Biotechnol. 15 (1987), p. 107.
[32] S. Lemieux and R. Carpentier, Photochem. Photobiol. 48 (1988), p. 115.
[33] D. Laberge, R. Rouillon and R. Carpentier, Enz. Microb. Technol. 26 (2000), p. 332.
[34] C. Loranger and R. Carpentier, Biotechnol. Bioeng. 44 (1994), p. 178.
[35] F. Bettazzi, L. Laschi and M. Mascini, Anal. Chim. Acta 589 (2007), p. 14.
[36] J. Lu and Z. Zhang, Anal. Chim. Acta 318 (1996), p. 175.
[37] G. He, H. Peng, T. Liu, M. Yang, Y. Zhang and Y. Fang, J. Mater. Chem. 19 (2009), p. 7347.
[38] G.M. Morris, D.S. Goodsell, R.S. Halliday, R. Huey, W.E. Hart, R.K. Belew and A.J. Olson, J. Comp. Chem. 19 (1998), p. 1639.
[39] R. Huey, G.M. Morris, A.J. Olson and D.S. Goodsell, J. Comp. Chem. 28 (2007), p. 1145.
[40] A. Guskov, J. Kern, A. Gabdulkhakov, M. Broser, A. Zouni and W. Saenger, Nat. Struct. Mol. Biol. 16 (2009), p. 334.
[41] E.P. Fuerst and M.A. Norman, Weed Sci. 39 (1991), p. 458.
[42] K.N. Ferreira, T.M. Iverson, K. Maghlaoui, J. Barber and S. Iwata, Science 303 (2004), p. 1831.
[43] J.J.S. van Rensen, Physiol. Plant 54 (1982), p. 515.
[44] W. Draber, K. Tietjen, J.F. Kluth and A. Trebst, Angew. Chem. Int. Ed. 30 (1991), p. 1621.
[45] A. Trebst and W. Draber, In Advances in Pesticide Science, Part 2, Pergamon, Oxford (1979) p. 223.
[46] A. Trebst, Methods Enyzmol. 69 (1980), p. 675.
[47] C.J. Arntzen, C.L. Ditto and P.E. Brewer, Proc. Natl. Acad. Sci., USA 76 (1979), p. 278.
[48] O. Kaminskaya, V.A. Shuvalov and G. Renger, Biochemistry 46 (2007), p. 1091.
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