Anil, Arga C., Shivaprakash, K. Nagaraju, Ramesha, B. Thimmappa, Uma Shaanker, Ramanan, Dayanandan, Selvadurai and Ravikanth, Gudasalamani (2014) Genetic Structure, Diversity and Long Term Viability of a Medicinal Plant, Nothapodytes nimmoniana Graham. (Icacinaceae), in Protected and Non-Protected Areas in the Western Ghats Biodiversity Hotspot. PLoS ONE, 9 (12). e112769. ISSN 1932-6203
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Official URL: http://dx.doi.org/10.1371/journal.pone.0112769
Abstract
Background and Question
The harvesting of medicinal plants from wild sources is escalating in many parts of the world, compromising the long-term survival of natural populations of medicinally important plants and sustainability of sources of raw material to meet pharmaceutical industry needs. Although protected areas are considered to play a central role in conservation of plant genetic resources, the effectiveness of protected areas for maintaining medicinal plant populations subject to intense harvesting pressure remain largely unknown. We conducted genetic and demographic studies of Nothapodytes nimmoniana Graham, one of the extensively harvested medicinal plant species in the Western Ghats biodiversity hotspot, India to assess the effectiveness of protected areas in long-term maintenance of economically important plant species.
Methodology/Principal Findings
The analysis of adults and seedlings of N. nimmoniana in four protected and four non-protected areas using 7 nuclear microsatellite loci revealed that populations that are distributed within protected areas are subject to lower levels of harvesting and maintain higher genetic diversity (He = 0.816, Ho = 0.607, A = 18.857) than populations in adjoining non-protected areas (He = 0.781, Ho = 0.511, A = 15.571). Furthermore, seedlings in protected areas had significantly higher observed heterozygosity (Ho = 0.630) and private alleles as compared to seedlings in adjoining non-protected areas (Ho = 0.426). Most populations revealed signatures of recent genetic bottleneck. The prediction of long-term maintenance of genetic diversity using BOTTLESIM indicated that current population sizes of the species are not sufficient to maintain 90% of present genetic diversity for next 100 years.
Conclusions/Significance
Overall, these results highlight the need for establishing more protected areas encompassing a large number of adult plants in the Western Ghats to conserve genetic diversity of economically and medicinally important plant species.
| Divisions: | Concordia University > Faculty of Arts and Science > Biology |
|---|---|
| Item Type: | Article |
| Refereed: | Yes |
| Authors: | Anil, Arga C. and Shivaprakash, K. Nagaraju and Ramesha, B. Thimmappa and Uma Shaanker, Ramanan and Dayanandan, Selvadurai and Ravikanth, Gudasalamani |
| Journal or Publication: | PLoS ONE |
| Date: | 2014 |
| Funders: |
|
| Digital Object Identifier (DOI): | 10.1371/journal.pone.0112769 |
| ID Code: | 982233 |
| Deposited By: | Danielle Dennie |
| Deposited On: | 17 Mar 2017 19:45 |
| Last Modified: | 18 Jan 2018 17:54 |
References:
1. Nantel P, Gagnon D, Nault AA (1996) Population viability analysis of American ginseng and wild leek harvested in stochastic environments. Conservation Biology 10:608–621.2. Sheldon JW, Balick MJ, Laird SA (1997) Medicinal plants: can utilization and conservation coexist? Advances in Economic Botany, vol. 12. 104p, New York Botanical Garden, Bronx, New York, USA.
3. Laurance WF (1999) Reflections on the tropical deforestation crisis. Biological Conservation 91:109–117.
4. Pearce F (1997) Herbal cures means plants suffer. New Scientist 15:6.
5. Ghimire KB, Pimbert MP Eds., Social Change and Conservation: Environmental Politics and Impacts of National Parks and Protected Areas (Earthscan, London, 1997).
6. Rodrigues ASL, Andelman SJ, Bakarr MI, Boitani L, Brooks TM, et al. (2004) Effectiveness of the global protected area network in representing species diversity. Nature 428:640–643.
7. Ehrlich PR, Pringle RM (2008) Where does biodiversity go from here? A grim business-as-usual forecast and a hopeful portfolio of partial solutions. Proceedings of the National Academy of Sciences USA 105:11579–11586.
8. Thapa S, Chapman DS (2009) Impacts of resource extraction on forest structure and diversity in Bardia National Park, Nepal. Forest Ecology and Management 259:641–649.
9. Leroux SJ, Krawchuk MA, Schmiegelow F, Cumming SG, Lisgo K, et al. (2010) Global protected areas and IUCN designations: Do the categories match the conditions? Biological Conservation. 143:609–616.
10. Bruner AG, Gullison RE, Rice RE, Da Fonseca GAB (2001) Effectiveness of parks in protecting tropical biodiversity. Science 291:125–128.
11. Uma Shaanker R, Ganeshaiah KN, Krishnan S, Ramya R, Meera C, et al. (2004) Livelihood gains and ecological costs of non-timber forest product dependence: assessing the roles of dependence, ecological knowledge and market structure in three contrasting human and ecological settings in South India. Environmental Conservation 31:242–253.
12. Nageswara Rao M, Uma Shaanker R, Ganeshaiah KN (2001) Mapping genetic diversity of sandal (Santalum album L.) in South India: Lessons for in-situ conservation of sandal genetic resources. In: Uma Shaanker R, Ganeshaiah KN, Bawa KS. (eds) Forest genetic resources: Status, threats and conservation strategies. Oxford and IBH Publishing Company Private Limited, New Delhi, India, 49–67.
13. Ramesha BT, Ravikanth G, Nageswara Rao M, Ganeshaiah KN, Uma Shaanker R (2007) Genetic structure of rattan, Calamus thwaitesii in core, buffer and peripheral regions of three protected areas at central Western Ghats, India: Do protected areas serve as refugia for genetic resources of economically important plants? Journal of Genetics 86:9–18.
14. Ravikanth G, Nageswara Rao M, Ganeshaiah KN, Uma Shaanker R (2009) Impacts of harvesting on genetic diversity of NTFP species: Implications for conservation. In: Uma Shaanker R, Joseph GC, Hiremath AJ (eds.) Management, utilization, and conservation of non-timber forest products in the South Asia region. Universities Press, Bangalore, India, 53–63.
15. Nageswara Rao M, Ravikanth G, Ganeshaiah KN, Uma Shaanker R (2010) Role of protected area in conserving the population and genetic structure of economically important bamboo species, In: Nageswara Rao M, Soneji JR (eds) Tree and forest biodiversity. Bioremediation, Biodiversity and Bioavailability 4:69–76.
16. Uma Shaanker R, Ramesha BT, Ravikanth G, Gunaga R, Vasudeva R, et al.. (2008) Chemical Profiling of Nothapodytes nimmoniana for Camptothecine, an Important Anticancer Alkaloid: Toward the Development of a Sustainable Production System. Pp 197–213. In Bioactive Molecules and Medicinal Plants (ed. Ramawat KG, Merillon JM) Springer Publishing, UK.
17. Raskin I, Ribnicky DM, Momarnytsky S, Ilic N, Poulev A, et al. (2002) Plants and human health in the twenty first century, Trends In Biotechnology. 20:522–531.
18. Kumar R, Ved DK (2000) 100 Red listed medicinal plants of conservation concern in Southern India, foundation for revitalization of local health traditions, Bangalore, 261–263.
19. Gowda HHC, Vasudeva R, Mathachen GP, Shaanker UR, Ganeshaiah KN (2002) Breeding types in Nothapodytes nimmoniana Graham. Current Science 83:1077–1078.
20. Ved DK (1997) Trade in medicinal plants: the state of our ignorance. Amruth 1:2–8.
21. Mittermeier RA, Patricio RG, Hoffman M, Pilgrim J, Brooks T, et al.. (2005) Hotspots revisited: earth’s biologically richest and most endangered terrestrial ecoregions. Conservation International. 392 p.
22. NWDC (National Wildlife Database Centre). 1999 Wild Life Institute of India, the report. Dehra Dun, India.
23. Ramesha BT, Amna T, Ravikanth G, Gunaga RP, Vasudeva R, et al. (2008) Prospecting for Camptothecinees from Nothapodytes nimmoniana in theWestern Ghats, South India: Identification of High-Yielding Sources of Camptothecine and New Families of Camptothecinees. Journal of Chromatographic Science 46:362–368.
24. Yamazaki Y, Urano A, Sudo H, Kitajima M, Takayama H, et al. (2003) Metabolite profiling of alkaloids and strictosidine synthase activity in Camptothecine producing plants. Phytochemistry 62:461–470.
25. Sharma MV, Uma Shaanker R, Leather SR, Vasudeva R, Shivanna KR (2011) Floral resources, pollinators and fruiting in a threatened tropical deciduous tree. Journal of Plant Ecology 4(4):259–267.
26. Ganeshaiah KN, Uma Shaanker R, Murali KS, Uma Shanker A, Bawa KS (1998) Extraction of non-timber forest products in the forest of Biligiri Rangan Hills. 5. Influence of dispersal mode on species response to anthropogenic pressures. Economic Botany 52:316–319.
27. Siegel S, Castellann JJ (1988) Nonparametric statistics for the behaviour sciences. McGraw-Hill, New York.
28. Doyle JJ, Doyle JS (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phyotochemical Bulletin 19:11–15.
29. Ramesha BT, Srirama R, Ravikanth G, Ravishankar KV, Dayanandan S, et al. (2008) Development of polymorphic microsatellite loci in Nothapodytes nimmoniana, a medicinally important tree from the Western Ghats, India. Molecular Ecology Resources 8(1):365–367.
30. Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. Journal of Heredity 86:248–249.
31. Leberg PL (2002) Estimating allelic richness: effects of sample size and bottlenecks. Molecular Ecology 11:2445–2449.
32. El Mousadik A, Petit RJ (1996) High level of genetic differentiation for allelic richness among populations of the argan tree [Argania spinosa (L) Skeels] endemic to Morocco. Theoretical Applied Genetics 92:832–839.
33. Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available from http://www.unil.ch/izea/softwares/fstat.html. Updated from Goudet J (1995) FSTAT v-1.2. A computer program to calculate F-statistics. Journal of Heredity 86:485–6.
34. Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1:47–50.
35. Guo SW, Thompson EA (1992) Performing the exact test of Hardy-Weinberg proportions for multiple alleles. Biometrics 48:361–372.
36. Kimura M, Crow J (1964) The number of alleles that can be maintained in finite populations. Genetics 49:725–738.
37. Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38:1358–1370.
38. Kimura M, Otha T (1978) Stepwise mutation model and distribution of allelic frequencies in finite populations. Proceedings of National Academy of Science. USA 75:2868–2872.
39. Slatkin M (1995) A measure of population subdivision based on microsatellite allele frequencies. Genetics 139:457–462.
40. Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics. 155:945–959.
41. Evanno G, Regnaut S, Goudet J (2007) Detecting the number of clusters of individuals using the software structure: a simulation study. Molecular Ecology 14:2611–2620.
42. Earl DA, Von Holdt BM (2011) Structure Harvester: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conservation Genetics Resources 4:359–361.
43. Jakobsson M, Rosenberg NA (2007) CLUMPP: A cluster matching and permutation program for dealing with label switching and multimodality in analysis of population structure. Bioinformatics 23:1801–1806.
44. Luikart G, Cornuet JM (1998) Empirical evaluation of a test for identifying recently bottlenecked populations from allele frequency data. Conservation Biology 12:228–237.
45. Frankham R, Ballou JD, Briscoe DA (2002) Introduction to conservation genetics. Cambridge (UK), Cambridge University Press.
46. Cornuet JM. Luikart (1996) Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics 144:2001–2014.
47. Piry S, Luikart G, Cornuet JM (1999) bottleneck: a computer program for detecting recent reductions in the effective population size using allele frequency data. Journal of Heredity 90:502–503.
48. Kuo CH, Janzen FJ (2003) BOTTLESIM: a bottleneck simulation program for long-lived species with overlapping generations. Molecular Ecology Notes 3:669–673.
49. Lemes MR, Gribel R, Proctor J, Grattapaglia D (2003) Population genetic structure of mahogany (Swietenia macrophylla King, Meliaceae) across the Brazilian Amazon based on variation at microsatellite loci, implications for conservation. Molecular Ecology 12:2845–2883.
50. Novick RR, Dick CD, Lemes MR, Navarro C, Caccon A, et al. (2003) Genetic structure of Mesoamerican population of big-leaf mahogany (Swietenia macrophylla) inferred from microsatellite analysis. Molecular Ecology 12:2885–2893.
51. Collevatti RG, Grattapaglia D, Hay JD (2003) Evidence for multiple lineages of Caryocar brasiliense populations in the Brazilian Cerrado based on the analysis of chloroplast DNA sequence and microsatellite haplotype variation. Molecular Ecology 12:105–115.
52. Christine O, Sokpon N, Khasa DP (2009) Genetic diversity and population structure of a threatened African tree species, Milicia excelsa, using nuclear microsatellites DNA markers. International Journal of Forestry Research Article ID 210179, 8 pages.
53. Ng KKS, Lee SL, Saw LG, Plotkin JB, Koh CL (2006) Spatial structure and genetic diversity of three tropical tree species with different habitat preferences within a natural forest. Tree Genetics and Genome 2:121–131.
54. Andrianoelina O, Favreau B, Ramamonjisoa L, Bouvet JM (2009) Small effect of fragmentation on the genetic diversity of Dalbergia monticola, an endangered tree species of the eastern forest of Madagascar, detected by chloroplast and nuclear microsatellites. Annals of Botany 104:1231–1242.
55. Zucchi MI, Rosana PVB, Pinheiro JB, Chaves LJ, Coelho ASG, et al. (2003) Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers. Genetics and Molecular Biology 26(4):449–457.
56. Dick CW, Hardy OJ, Jones FA, Petit RA (2008) Spatial scales of pollen and seed-mediated gene flow in tropical rain forest trees. Tropical Plant Biology 1:20–33.
57. Guidugli MC, Accoroni G, Mestriner MA, Betioli Contel EA, Martinez CA, et al. (2010) Genetic characterization of 12 heterologous microsatellite markers for the giant tropical tree Cariniana legalis (Lecythidaceae). Genetics and Molecular Biology 33(1):131–134.
58. Sharma MV, Shaanker RU, Vasudeva R, Shivanna KR (2010) Functional dioecy in Nothapodytes nimmoniana, a distylous species in the Western Ghats. Current Science 99:1444–1449.
59. Tadwalkar MD, Joglekar AM, Mhaskar M, Kanade RB, Chavan B, et al. (2012) Dispersal modes of woody species from the northern Western Ghats, India. Tropical Ecology 53:53–67.
60. Aldrich PR, Hamrick JL, Chavarriaga P, Kochert G (1998) Microsatellite analysis of demographic genetic structure in fragmented populations of the tropical tree Symphonia globulifera. Molecular Ecology 7:933–944.
61. Dayanandan S, Dole J, Bawa KS, Kesseli R (1999) Population structure delineated with microsatellite markers in fragmented populations of a tropical tree, Carapa guianensis (Meliaceae). Molecular Ecology 8:1585–1592.
62. White CM, Boshier DH, Powell W (1999) Genetic variation within a fragmented population of Swietenia humilis Zucc. Molecular Ecology 11:1899–1910.
63. Hamrick JL, Godt MJ (1996) Effects of life history traits on genetic diversity in plant species. Philosophical Transactions of the Royal Society of London Series B 351:1291–1298.
64. Abdul Kareem VK, Rajasekharan PE, Mini S, Kumar VT (2011) Genetic diversity and structure of the threatened anti-cancerous plant Nothapodytes nimmoniana as revealed by ISSR analysis. Plant Genetic Resources 9(4):506–514.
65. Brossart JL, Prowell DP (1998) Genetic estimates of population structure and gene flow: limitations, lessons, and new directions. Trends in Ecology and Evolution 13:202–206.
66. Sork VL, Nason J, Campbell DR, Fernandez JF (1999) Landscape approaches to historical and contemporary gene flow in plants. Trends in Ecology and Evolution 14:219–223s.
67. Whitlock MC, Mccauley DE (1999) Indirect measures of gene flow and migration: FST ≠ 1/(4Nm+1). Heredity 82:117–125.
68. Savolainen O, Kuittinen H (2000) Small population processes. In: Young A, Boshier D, Boyle T. eds. Forest conservation genetics, principles and practice. Wallingford: CSIRO Publishing, 91–100.
69. Shivaprakash KN (2011) Population genetics of vulnerable and endemic tree species Myristica malabarica and evaluation of ecological niche models (ENMs). Master Thesis submitted to University of Agricultural Sciences, Bangalore, India.
70. Loiselle BA, Sork VL, Nason J, Graham C (1995) Comparison of genetic variation in bird-dispersed shrubs of a tropical wet forest. Biotropica 27:487–494.
71. Chung M, Chung MY, Oh G, Epperson B (2000) Spatial genetic structure in a Neolitsea sericea population (Lauraceae). Heredity 85:490–497.
72. Lee CT, Wickneswari R, Mahani MC, Zakri AH. 2002 Effect of selective logging on the genetic diversity of Scaphium macropodum. Biological Conservation 104:107–118.
73. Schemske DW, Husband BC, Ruckelshaus MH, Goodwillie C, Parker IM, et al. (1994) Evaluating approaches to the conservation of rare and endangered plants. Ecology 75:584–606.
74. Hundertmark KJ, Van Daele LJ (2010) Founder effect and bottleneck signatures in an introduced, insular population of elk. Conservation Genetics 11:139–147.
75. Lippe CA, Dumont P, Bernatchez L (2006) High genetic diversity and no inbreeding in the endangered copper redhorse, Moxostoma hubbsi (Catostomidae, Pisces): the positive sides of a long generation time. Molecular Ecology 15:1769–1780.
76. Vrijenhoek RC, Douglas ME, Meffe GK (1985) Conservation genetics of endangered fish populations in Arizona. Science 229:400–402.
77. Cruse-Sanders JM, Hamrick JL (2004) Spatial and genetic structure within populations of wild American ginseng (Panax quinquefolius L., Araliaceae). Journal of Heredity 95:309–321.
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