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The Interplay between the Circadian and Stress Systems: How does Early Environmental Impoverishment Impact Circadian Phenotype and Clock Gene Expression?

Title:

The Interplay between the Circadian and Stress Systems: How does Early Environmental Impoverishment Impact Circadian Phenotype and Clock Gene Expression?

Benoit, Miranda (2018) The Interplay between the Circadian and Stress Systems: How does Early Environmental Impoverishment Impact Circadian Phenotype and Clock Gene Expression? Masters thesis, Concordia University.

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Abstract

The circadian system and the stress system each impact an organism’s ability to adapt to environmental challenges (Weibel, Maccari, Van Reeth, 2002). Within the field of circadian studies, much research has been conducted examining the way in which stressors affect clock gene regulation and circadian phenotype. Despite this, very few studies, if any, have looked at the role of early social and environmental impoverishment on circadian phenotype and clock gene expression. As a result, the present study explored whether there was a difference in circadian phenotype and clock gene expression between two groups of rats, one of which had experienced typical early social and physical enrichment, and the other that had been raised in a socially and physically impoverished environment. It was hypothesized that animals that had been raised in an impoverished environment would have a less robust circadian rhythm and be more prone to negative perturbations to both the circadian and stress systems following an acute stressor. Though there was a trend towards significant differences between treatment groups for wheel running behaviours in experiment 1, the only treatment effects found were those of PER1 expression in the amygdala and hippocampus. In experiment 2 a novelty-induced locomotor test was administered to see if the treatment had been effective, and there was a significant main effect of treatment condition. Furthermore, a significant difference between treatment conditions was seen again in the hippocampus. Beyond these treatment effects, there were multiple significant main effects of both sex and ZT for nearly all tests carried out in both experiments.

Divisions:Concordia University > Faculty of Arts and Science > Psychology
Item Type:Thesis (Masters)
Authors:Benoit, Miranda
Institution:Concordia University
Degree Name:M.A.
Program:Psychology
Date:20 December 2018
Thesis Supervisor(s):Amir, Shimon
Keywords:circadian, stress, development, corticosterone, impoverishment, early environment
ID Code:984817
Deposited By: Miranda Benoit
Deposited On:17 Jun 2019 16:39
Last Modified:16 Feb 2021 23:36

References:

Ahern, M., Goodell, D. J., Adams, J., & Bland, S. T. (2016). Brain regional differences in social encounter-induced Fos expression in male and female rats after post-weaning social isolation. Brain Research, 1630, 120-133. doi: 10.1016/j.brainres.2015.11.006
Al-Safadi, S., Al-Safadi, A., Branchaud, M., Rutherford, S., Dayanandan, A., Robinson, B., … Yamazaki, S. (2014). Stress-induced changes in the expression of the clock protein PERIOD1 in the rat limbic forebrain and hypothalamus: Role of stress type, time of day, and predictability. PLoS One, 9. doi: 10.1371/journal. pone.0111166
Al Safadi, S., Branchaud, M., Rutherford, S., & Amir, S. (2015). Glucocorticoids and Stress-Induced Changes in the Expression of PERIOD1 in the Rat Forebrain. PLoS ONE, 10, 1-13. doi: 10.1371/journal.pone.0130085
Amir, S., Lamont, E.W., Robinson, B. & Stewart, J. (2004) A circadian rhythm in the expression of PERIOD2 protein reveals a novel SCN-controlled oscillator in the oval nucleus of the bed nucleus of the stria terminalis. Journal of Neuroscience, 24, 781-790. doi: 10.1523/JNEUROSCI.4488-03.2004
Anyan, J., & Amir, S. (2018). Too Depressed to Swim or Too Afraid to Stop? A Reinterpretation of the Forced Swim Test as a Measure of Anxiety-Like Behaviour. Neuropsychopharmacology, 43, 931-933. doi: 10.1038/npp.2017.260
Babb, J.A., Masini, C.V., Day, H.E.W., & Campeau, S. (2014). Habituation of hypothalamic-pituitary-adrenocortical axis hormones to repeated homotypic stress and subsequent heterotypic stressor exposure in male and female rats. Stress, 17, 224–234. doi: 10.3109/ 10253890.2014.905534
Baldini, S., Restani, L., Baroncelli, L., Coltelli, M., Franco, R., Cenni, M. C., & ... Berardi, N. (2013). Enriched early life experiences reduce adult anxiety-like behavior in rats: A role for insulin-like growth factor 1. The Journal of Neuroscience, 33, 11715-11723. doi:10.1523/JNEUROSCI.3541-12.2013
Balsalobre, A., Brown, S.A., Marcacci, L., Tronche, F., Kellendonk, C., Reichardt, H.M., … Schibler, U. (2000). Resetting of circadian time in peripheral tissues by glucocorticoid signaling. Science, 289, 2344–2347. doi: 10.1126/science.289.5488.2344
Bowling, S.L., & Bardo, M.T. (1994). Locomotor and rewarding effects of amphetamine in enriched, social, and isolate reared rats. Pharmacology Biochemistry Behavior, 48, 459–464.
Christiansen, S., Bouzinova, E. V., Palme, R., & Wiborg, O. (2012). Circadian activity of the
hypothalamic–pituitary–adrenal axis is differentially affected in the rat chronic mild stress model of depression. Stress: The International Journal on The Biology of Stress, 15, 647-657. doi:10.3109/10253890.2011.654370
Chun, L. E., Christensen, J., Woodruff, E. R., Morton, S. J., Hinds, L. R., & Spencer, R. L. (2018). Adrenal-dependent and -independent stress-induced Per1 mRNA in hypothalamic paraventricular nucleus and prefrontal cortex of male and female rats. Stress: The International Journal on The Biology of Stress, 21, 69-83. doi:10.1080/10253890.2017.1404571
Cora, M. C., Kooistra, L., & Travlos, G. (2015). Vaginal Cytology of the Laboratory Rat and Mouse: Review and Criteria for the Staging of the Estrous Cycle Using Stained Vaginal Smears. Toxicologic Pathology, 43, 776-793. doi: https://doi.org/10.1177%2F0192623315570339
Emens, J. S., Yuhas, K., Rough, J., Kochar, N., Peters, D., Lewy, A. J. (2009). Phase angle of entrainment in morning- and evening-types under naturalistic conditions. Chronobiology International, 26, 474-493. doi: 10.1080/07420520902821077
Fries, E., Hesse, J., Hellhammer, J., Hellhammer, D. H. (2005). A new view on hypocortisolism.
Psychoneuroendocrinology, 30, 1010-1016. doi: 10.1016/j.psyneuen.2005.04.006
Gomez-Sanchez, E., & Gomez-Sanchez, C. E. (2014). The multifaceted mineralocorticoid receptor. Comprehensive Physiology, 4, 965-994. doi: 10.1002/cphy.c130044
Goriki, A., Hatanaka, F., Myung, J., Kim, J. K., Yoritaka, T., Tanoue, S., & ... Takumi, T.
(2014). A novel protein, CHRONO, functions as a core component of the mammalian circadian clock. Plos Biology, 12, doi: 10.1371/journal.pbio.1001839
Green, T.A., Cain, M.E., Thompson, M., & Bardo, M. T. (2003). Environmental enrichment decreases nicotine-induced hyperactivity in rats. Psychopharmacology, 170, 235–241.
Haim, S., Shakhar, G., Rossene, E., Taylor, A. N., & Ben-Eliyauh, S. (2003). Serum levels of sex hormones and corticosterone throughout 4- and 5-day estrous cycles in Fischer 344 rats and their simulation in ovariectomized females. Journal of Endocrinological Investigation, 26, 1013-1022. doi: 10.1007/BF03348201
Haupt, M., & Schaefers, A. T. U. (20 10). Effects of postweaning social and physical deprivation on locomotor activity patterns and explorative behavior in female CD‐1 mice. Developmental Psychobiology, 52, 383-393. doi: https://doi.org/10.1002/dev.20439
Heim, C., Ehlert, U., Hanker, J.P., & Hellhammer, D.H. (1998). Abuse-related posttraumatic stress disorder and alterations of the hypothalamic–pituitary–adrenal axis in women with chronic pelvic pain. Psychosomatic Medicine, 60, 309–318. doi: http://dx.doi.org/10.1097/00006842-199805000-00017
Heim, C., Ehlert, U., & Hellhammer, D.H. (2000). The potential role of hypocortisolism in the pathophysiology of stress-related bodily disorders. Psychoneuroendocrinology 25, 1–35. doi: https://doi.org/10.1016/S0306-4530(99)00035-9
Hellhammer, D.H., & Wade, S. (1993). Endocrine correlates of stress vulnerability. Psychotherapy and Psychosomatics, 60, 8–17. doi: https://doi.org/10.1159/000288675
Jagannath, A., Peirson, S. N., & Foster, R. G. (2013). Sleep and circadian rhythm disruption in neuropsychiatric illness. Current Opinion in Neurobiology, 23, 888-894. doi: http://dx.doi.org/10.1016/j.conb.2013.03.008
Jauregui Huerta et al. (2015). Hippocampal cytogenesis and spatial learning in senile rats
exposed to chronic variable stress: effects of previous early life exposure to mild stress.
Kanitz, Hameister, Tuchscherer, Tuchscherer, & Puppe. (2016). Social support modulates stress-
related gene expression in various brain regions of piglets
Koch, C. E., Leinweber, B., Drengberg, B. C., Blaum, C., & Oster, H. (2017). Interaction between circadian rhythms and stress. Neurobiology of Stress, 657-67. doi: 10.1016/j.ynstr.2016.09.001
Lebow, M. A., & Chen, A. (2016). Overshadowed by the amygdala: the bed nucleus of the stria terminalis emerges as key to psychiatric disorders. Molecular Psychiatry, 21, 450-463. doi: 10.1038/mp.2016.1
Logan, R. W., Edgar, N., Gillman, A. D., Hoffman, D., Zhu, X., & McClung, C. A. (2015). Chronic stress induces brain region-specific alterations of molecular rhythms that correlate with depression-like behavior in mice. Biological Psychiatry, 78, 249-258. doi: http://dx.doi.org/10.1016/j.biopsych.2015.01.011
Lovick, T. A. (2012) Estrous cycle and stress: Influence of progesterone on the female brain. Brazilian Journal of Medical and Biological Research, 45, 314–320.
McLean, C. P., Asnaani, A., Litz, B. T., & Hofmann, S. G. (2011). Gender differences in anxiety disorders: Prevalence, course of illness, comorbidity and burden of illness. Journal Of Psychiatric Research, 45, 1027-1035. doi: 10.1016/j.jpsychires.2011.03.006
McClung, C. A. (2013). How might circadian rhythms control mood? Let me count the ways…. Biological Psychiatry, 74, 242-249. doi: https://doi.org/10.1016/j.biopsych.2013.02.019
Mifsud, K. R. & Reul, J. M. H. M. (2016). Acute stress enhances heterodimerization and binding
of corticosteroid receptors at glucocorticoid target genes in the hippocampus. Proceedings of the National Academy of Sciences of the United States of America, 113, 11336- 11341. doi: http://dx.doi.org/10.1073/pnas.1605246113
Mourlon, V., Naudon, L., Giros, B., Crumeyrolle-Arias, M., & Daugé, V. (2011). Early stress leads to effects on estrous cycle and differential response to stress. Physiology and Behavior, 102, 304-310. doi: 10.1016/j.physbeh.2010.11.003
Niknazar, S., Nahavandi, A., Peyvandi, A.A. et al. (2016). Comparison of the adulthood chronic stress effect on hippocampal BDNF signaling in male and female rats. Molecular Neurobiology, 53, 4026-4033. doi: http://dx.doi.org/10.1007/s12035-015-9345-5
Oster, H., Challet, E., Ott, V., Arvat, E., de Kloet, E. R., Dijk, D. J., . . . Van Cauter, E. (2017). The functional and clinical significance of the 24-h rhythm of circulating glucocorticoids.
Oyola, M. G., & Handa, R. J. (2017). Hypothalamic–pituitary–adrenal and hypothalamic–pituitary–gonadal axes: Sex differences in regulation of stress responsivity. Stress: The International Journal On The Biology Of Stress, 20, 476-494. doi:10.1080/10253890.2017.1369523
Pesce, L., van Veen, T., Carlier, I., van Noorden, M. S., van der Wee, N. A., van Hemert, A. M., & Giltay, E. J. (2016). Gender differences in outpatients with anxiety disorders: The Leiden Routine Outcome Monitoring Study. Epidemiology And Psychiatric Sciences, 25, 278-287. doi:10.1017/S2045796015000414
Pittendrigh, C. S., & Daan, S. (1976). A functional analysis of circadian pacemakers in nocturnal rodents. Journal of Comparative Physiology, 106, 291-331.
Porsolt, R. D., Brossard, G., Hautbois, C., Roux, S. (2011). Rodent models of depression: forced swimming and tail suspension behavioral despair tests in rats and mice. Current Protocols in Neuroscience, 8. doi: 10.1002/0471142301.ns0810as55
Rasmussen, I. S., Arefjord, K., Winje, D., & Dovran, A. (2018). Childhood maltreatment trauma: A comparison between patients in treatment for substance use disorders and patients in mental health treatment. European Journal of Psychotraumatology, 9, 1-10. doi: 10.1080/20008198.2018.1492835
Romeo, R. D., Bellani. R., Karatsoreos, I. N., Chhua, N., Vernov, M., Conrad, C. D., & McEwen, B. S. (2006). Stress history and pubertal development interact to shape hypothalamic-pituitary-adrenal axis plasticity. Endocrinology, 147, 1664–1674. doi: 10.1210/en.2005-1432
Romeo, R. D., Lee, S. J, & McEwen, B. S. (2004). Differential stress reactivity in intact and ovariectomized prepubertal and adult female rats. Neuroendocrinology, 80, 387–393. doi: 10.1159/000084203
Sáenz, J. C. B., Villagra, O. R., & Trías, J. F. (2006). Factor analysis of Forced Swimming test, Sucrose Preference test and Open Field test on enriched, social and isolated reared rats. Behavioural Brain Research, 169, 57-65. doi: 10.1016/j.bbr.2005.12.001
Segall, L.A. and Amir, S. (2010). Glucocorticoid regulation of clock gene expression in the mammalian limbic forebrain. Journal of Molecular Neuroscience, 42, 168-175.
Segall, L. A., Milet, A., Tronche, F., & Amir, S. (2009). Brain glucocorticoid receptors are necessary for the rhythmic expression of the clock protein, PERIOD2, in the central extended amygdala in mice. Neuroscience Letters, 457, 58-60. https://doi.org/10.1016/j.neulet.2009.03.083
Segall, L.A., Perrin, J.S., Walker, C.D., Stewart, J. and Amir, S. (2006). Glucocorticoid rhythms control the rhythm of expression of the clock protein, PERIOD2, in oval nucleus of the bed nucleus of the stria terminalis and central nucleus of the amygdala in rats. Neuroscience, 140, 753-757.
Smith, S. M., & Vale, W. W. (2006). The role of the hypothalamic-pituitary-adrenal axis in neuroendocrine response to stress. Dialogues in Clinical Neuroscience, 8, 383-395.
Spencer, S., Falcon, E., Kumar, J., Krishnan, V., Mukherjee, S., Birnbaum, S. G., & McClung, C. A. (2013). Circadian genes Period1 and Period2 in the nucleus accumbens regulate anxiety-like behavior. European Journal of Neuroscience, 37, 242-250. doi: 10.1111/ejn.12010.
Staley, K., & Scharfman, H. (2005). A women’s prerogative. Nature Neuroscience, 8, 697-699. doi: https://doi.org/10.1038/nn0605-69710.1038/nn0605-697
Sterlemann, V., Ganea, K., Liebl, C., Harbich, D., Alam, S., Holsboer, F., & ... Schmidt, M. V. (2008). Long-term behavioral and neuroendocrine alterations following chronic social stress in mice: Implications for stress-related disorders. Hormones and Behavior, 53, 386-394. doi: 10.1016/j.yhbeh.2007.11.001
Stress [Def. ]. (n.d.) In APA Dictionary Online, Retrieved January 2, 2017, from
http://psycnet.apa.org/index.cfm?fa=termfinder.displayTerm&query=50170
Verma, P., Hellemans, K. G. C., Choi, F. Y., Yu, W., & Weinberg, J. (2010). Circadian phase and sex effects on depressive/anxiety-like behaviors and HPA axis responses to acute stress. Physiology & Behavior, 99, 276-285. doi: 10.1016/j.physbeh.2009.11.002
Verwey, M., Dhir, S., & Amir, S. (2016). Circadian influences on dopamine circuits of the brain: Regulation of striatal rhythms of clock gene expression and implications for psychopathology and disease. F1000research, 5. doi: 10.12688/f1000research.9180.1
Verwey, M., Robinson, B., Amir, S. (2013) Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents. Journal of Visualized Experiments, 71. doi:10.3791/50186
Viau, V., & Meaney, M. J. (1991). Variations in the hypothalamic-pituitary-adrenal response to stress during the estrous cycle in the rat. Endocrinology, 129, 2503–2511. doi: 10.1210/endo-129-5-2503
Weintraub, A., Singaravelu, J., & Bhatnagar, S. (2010). Enduring and sex-specific effects of adolescent social isolation in rats on adult stress reactivity. Brain Research, 134, 383-92. doi: 10.1016/j.brainres.2010.04.068
Yankelevitch-Yahav, R., Franko, M., Huly, A., Doron, R. The Forced Swim Test as a Model of Depressive-like Behavior. Journal of Visualized Experiments, 97. doi:10.3791/52587
Young, M. E., & Bray, M. S. (2007). Potential role for peripheral circadian clock dyssynchrony in the pathologies of cardiovascular dysfunction. Sleep Medcine, 8, 656-667. doi: 10.1016/j.sleep.2006.12.010
Zakharova, E., Starosciak, A., Wade, D., & Izenwasser, S. (2012). Sex differences in the effects of social and physical environment on novelty-induced exploratory behavior and cocaine-stimulated locomotor activity in adolescent rats. Behavioural Brain Research, 230, 92–99. doi: http://doi.org/10.1016/j.bbr.2012.01.052
Zhang, J., Wu, Z., Zhou, L., Li, H., Teng, H., Dai, W., … Sun, Z. S. (2011). Deficiency of antinociception and excessive grooming induced by acute immobilization stress in Per1 mutant mice. PLoS One, 6, 1-9. doi: https://doi.org/10.1371/journal.pone.0016212
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